Product Information
Reconstitution:
With carrier: Add sterile PBS or PBS containing 1% bovine or human serum albumin or 5-10% FBS to a final mSCF concentration of greater than 50 μg/ml. Solubilize for 30 minutes at room temperature with occasional gentle vortexing.
Carrier free: Add sterile PBS or PBS containing protein to minimize absorption of mSCF to surfaces. Solubilize for 30 minutes at room temperature with occasional gentle vortexing. Stock mSCF should be greater than 50 μg/ml.
MW (kDa) | 22-35 |
Purity | >98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant mSCF. All lots are greater than 98% pure. |
Endotoxin | Less than 0.01 ng endotoxin/1 μg mSCF. |
Activity | The bioactivity of recombinant mSCF was determined in an M-07e cell proliferation assay. The ED50 of each lot is between 2-8 ng/ml. |
Molecular Formula | Recombinant mSCF contains no "tags" and the nonglycosylated protein has a calculated MW of 18,298. DTT-reduced and non-reduced protein migrate as 22-35 kDa polypeptides. Lower mobility and heterogeneity in SDS-PAGE are due to glycosylation. The expected amino-terminal KEICG of recombinant mSCF was verified by amino acid sequencing. |
Recombinant mouse SCF (mSCF) Lys26-Ala189 (Accession #NP_038626) was expressed in human 293 cells at Cell Signaling Technology.
SCF is produced by endothelial cells, fibroblasts, keratinocytes, gut epithelial cells, and tumor cells (1,2). SCF is critical for hematopoiesis and mast cell differentiation and has additional roles in survival and function of other cell types (1). Some tumor cell proliferation and invasiveness are promoted by SCF (3). Tumor-derived SCF appears to be involved in expansion of myeloid-derived suppressor cells which, in turn, limits proliferation of tumor-infiltrating T-cells (4). SCF may have additional roles in the tumor microenvironment (2). SCF is either soluble or an integral membrane protein and its form is dependant on variation in splicing or proteolytic release (1). SCF binds to the receptor tyrosine kinase c-Kit and induces activation of the Akt, Erk, JNK, and p38 pathways (5,6).
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Cat. # | Size | Price | Inventory |
---|---|---|---|
5223SC | 10 µg (With Carrier) | ||
5223SF | 10 µg (Carrier Free) |