Interested in promotions? | Click here >>
Annexin V-FITC Early Apoptosis Detection Kit

Annexin V-FITC Early Apoptosis Detection Kit #6592


Confocal immunofluorescent analysis of live Jurkat cells, untreated (left) or camptothecin-treated (right), using Annexin V-FITC Conjugate (green). Red = Propidium Iodide (fluorescent DNA dye). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Learn more about how we get our images
Flow Cytometry

Flow cytometric analysis of Jurkat cells untreated (left) or treated with camptothecin (10uM, 4 hr; right) using Annexin V-FITC Early Apoptosis Detection Kit.

Learn more about how we get our images
Product Includes Quantity (with Count)
Annexin V-FITC Conjugate 1 x 100 µl
10X Annexin V Binding Buffer 2 x 1.5 ml
Propidium Iodide (PI) Solution 1 x 1.3 ml

The Annexin V-FITC Early Apoptosis Detection Kit enables researchers to identify early apoptotic cells within a cell population. Annexin V-FITC conjugated protein binds to cell surfaces expressing phosphatidylserine, an early apotosis marker. Cells stained with propidium iodide (PI), a non-cell-permeable DNA dye, indicate necrotic cells. Cells stained with both PI and annexin V-FITC demonstrate later stage apoptosis and early necrosis. This kit provides enough reagent to perform 100 assays, based on a 250 μl assay volume.

Annexin V belongs to the annexin family of proteins consisting of over 160 members. All annexin family members share the same charactersistic of Ca2+-dependent binding to negatively charged phospholipid surfaces (1). During early apoptosis, phosphatidylserine (PS) is translocated from the cytosolic side of the plasma membrane to the cellular surface. This translocation exposes PS to the extracellular environment with the plasma membrane left intact (2). Annexin V protein demonstrates high affinity, specificity, and sensitivity for PS and can be used as a marker of early apoptosis. In order to rule out "leaky" necrotic cells, annexin V must be used in tandem with reagents that determine the integrity of the cell membrane, such as propidium iodide (2).

  1. Boersma, H.H. et al. (2005) J Nucl Med 46, 2035-50.
  2. Vermes, I. et al. (1995) J Immunol Methods 184, 39-51.
Entrez-Gene Id
Swiss-Prot Acc.
For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
DRAQ5 is a registered trademark of Biostatus Limited.
The Annexin V-FITC conjugate is a product manufactured for Cell Signaling Technology, Inc. by TAU Technologies BV.

Upstream / Downstream


Explore pathways related to this product.

To Purchase # 6592S

Product Number Size
6592S 1 Kit (100 assays)

Inventory Status

SKU 库存状态 Price

Your Local Representative for China (PRC)

CST (Shanghai) Biological Reagents Company Limited

Building 7

A-SUN Science & Technology Park

399 Shengxia Road

Pudong Shanghai 201210


4006-473287/GreatQ [Toll Free]

Need information for a different country? Please click here.

To get local purchase information on this product, click here.

Powered By OneLink