Cat. # | Size | Price | Inventory |
---|---|---|---|
31411S | 100 µl (50 tests) |
REACTIVITY | H M R Hm Mk B Dg |
SENSITIVITY | Endogenous |
MW (kDa) | |
Source/Isotype | Rabbit IgG |
Product Information
Application | Dilution |
---|---|
Immunofluorescence (Frozen) | 1:100 - 1:400 |
Immunofluorescence (Immunocytochemistry) | 1:50 - 1:100 |
Flow Cytometry (Fixed/Permeabilized) | 1:50 |
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalent grade water.
Cover sections with 4% formaldehyde dilute in 1X PBS.
NOTE: Formaldehyde is toxic, use only in fume hood.
NOTE: All subsequent incubations should be carried out at room temperature unless otherwise noted in a humid light-tight box or covered dish/plate to prevent drying and fluorochrome fading.
posted November 2006
revised November 2013
Protocol Id: 222
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalent grade water.
NOTE: Cells should be grown, treated, fixed and stained directly in multi-well plates, chamber slides or on coverslips.
Aspirate liquid, then cover cells to a depth of 2–3 mm with 4% formaldehyde diluted in 1X PBS.
NOTE: Formaldehyde is toxic, use only in a fume hood.
NOTE: All subsequent incubations should be carried out at room temperature unless otherwise noted in a humid light-tight box or covered dish/plate to prevent drying and fluorochrome fading.
posted November 2006
revised November 2013
Protocol Id: 182
All reagents required for this protocol may be efficiently purchased together in our Intracellular Flow Cytometry Kit (Methanol) #13593, or individually using the catalog numbers listed below.
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalent grade water.
NOTE: When including fluorescent cellular dyes in your experiment (including viability dyes, DNA dyes, etc.), please refer to the dye product page for the recommended protocol. Visit www.cellsignal.com for a full listing of cellular dyes validated for use in flow cytometry.
NOTE: Adherent cells or tissue should be dissociated and in single-cell suspension prior to fixation.
NOTE: Optimal centrifugation conditions will vary depending upon cell type and reagent volume. Generally, 150-300g for 1-5 minutes will be sufficient to pellet the cells.
NOTE: If using whole blood, lyse red blood cells and wash by centrifugation prior to fixation.
NOTE: Antibodies targeting CD markers or other extracellular proteins may be added prior to fixation if the epitope is disrupted by formaldehyde and/or methanol. The antibodies will remain bound to the target of interest during the fixation and permeabilization process. However, note that some fluorophores (including PE and APC) are damaged by methanol and thus should not be added prior to permeabilization. Conduct a small-scale experiment if you are unsure.
NOTE: Count cells using a hemocytometer or alternative method.
posted July 2009
revised June 2020
实验步骤编号:407
人, 小鼠, 大鼠, 仓鼠 , 猴, 牛 , 犬
使用与人小窝蛋白 1 中 Glu20 周围的残基相对应的合成肽,对动物进行免疫接种来产生单克隆抗体。
21-24 kDa 整合蛋白 caveolins 是胆固醇/神经鞘脂富含的质膜微区小窝的主要结构组分。根据不同的组织分布,已识别出小窝蛋白家族的三个成员(caveolin-1、2 和 3)。小窝蛋白可形成与胆固醇和其他脂质相互作用的异源和同源低聚体 (1)。小窝蛋白参与调节不同的生物功能,包括囊泡运输、胆固醇稳态、细胞黏附和凋亡,也和神经退行性疾病有关联 (2)。小窝蛋白和多种信号转导分子相互作用,例如 Gα 亚基、酪氨酸激酶受体、PKC、Src 家族酪氨酸激酶和 eNOS (1,2)。小窝蛋白被认为可作为融合信号转导的支架蛋白。小窝蛋白在 Tyr14 位点的磷酸化对小窝蛋白结合包含 SH2 或 PTB 结构域的接头蛋白非常重要,如 GRB7 (3-5)。Ser80 位点的磷酸化可调控小窝蛋白,使其与 ER 膜结合,然后进入分泌通路 (6)。
探索与本品相关的通路。
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