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Tyrphostin AG 1478
激活剂与抑制剂
化学调节物

Tyrphostin AG 1478 #9842

Citations (0)
Structure of Tyrphostin AG 1478
Western blot analysis of extracts from serum starved A431 cells, untreated or treated with indicated concentrations of Tyrphostin AG1478 for 30 minutes, followed by stimulation with EGF for 10 minutes. The EGFR activation was detected by using Phospho-EGF Receptor (Tyr1173) (53A5) Rabbit mAb #4407 (upper). EGF Receptor Antibody #2232 was used to detect the equal loading of EGFR (lower). Tyrphostin AG1478-mediated inhibition of EGF-induced phosphorylation of EGF Receptor at Tyr1173 is shown.

Product Usage Information

Tyrphostin AG 1478 is supplied as 400 μg powder. Store at or below -20ºC. Before use, dissolve powder in 1.23 ml DMSO to make a 1 mM

Tyrphostin AG 1478 stock solution. For working concentrations of 100 nM-200 nM, dilute DMSO stock 1:10,000 to 1:5,000. Treat cells with the desired concentration for 30 minutes.

Storage

Store lyophilized or in solution at -20ºC, desiccated. Protect from light. In lyophilized form, the chemical is stable for 24 months. Once in solution, use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.

Product Description

Molecular Weight 315.76 g/mol
Purity >99%
Molecular Formula C16H14CIN3O2
CAS 175178-82-2
Solubility Soluble in DMSO at 10mg/ml.

Background

Tyrphostin AG 1478 is a tyrosine kinase inhibitor specifically selective to EGFR (ErbB1), with an IC50 of about 3 nM in vitro (1,2). Treatment of cell with 50-150 nM of AG 1478 can substantially block EGFR activiation in vivo (3). In addition to EGFR, AG 1478 also inhibits ErbB4 activation induced by radiation in cancer cells (4). Testing of AG 1478 alone or in combination with other treatments to assess anti-tumor and anti-fibrotic effectiveness has yielded promising results (5-8).

Limited Uses

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For Research Use Only. Not for Use in Diagnostic Procedures.
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