Revision 1

#9765Store at -20C

1 Kit

(8 x 20 microliters)

Cell Signaling Technology

Orders: 877-616-CELL (2355) [email protected]

Support: 877-678-TECH (8324)

Web: [email protected] cellsignal.com

3 Trask LaneDanversMassachusetts01923USA
For Research Use Only. Not for Use in Diagnostic Procedures.
Product Includes Product # Quantity Mol. Wt Isotype/Source
Phospho-Caveolin-1 (Tyr14) Antibody 3251 20 µl 23, 25 kDa Rabbit 
Caveolin-1 (D46G3) XP® Rabbit mAb 3267 20 µl 21, 24 kDa Rabbit IgG
Clathrin Heavy Chain (D3C6) XP® Rabbit mAb 4796 20 µl 190 kDa Rabbit IgG
APPL1 (D83H4) XP® Rabbit mAb 3858 20 µl 82 kDa Rabbit IgG
EEA1 (C45B10) Rabbit mAb 3288 20 µl 170 kDa Rabbit IgG
Syntaxin 6 (C34B2) Rabbit mAb 2869 20 µl 32 kDa Rabbit IgG
Rab5A (E6N8S) Mouse mAb 46449 20 µl 25 kDa Mouse IgG1
GOPC (D10A12) Rabbit mAb 8576 20 µl 59 kDa Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl Goat 
Anti-mouse IgG, HRP-linked Antibody 7076 100 µl Horse 

Please visit cellsignal.com for individual component applications, species cross-reactivity, dilutions, protocols, and additional product information.

Description

The Vesicle Trafficking Antibody Sampler kit provides an economical means to analyze proteins involved in the intracellular transport of cargo proteins. This kit includes enough primary and secondary antibody to perform two western blot experiments.

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Background

Vesicle trafficking is an integral cellular process and the associated proteins involved also play major roles in other signaling pathways. Caveolins are involved in diverse biological functions including vesicular trafficking, cholesterol homeostasis, cell adhesion, apoptosis, and are also indicated in neurodegenerative disease (1). It is believed that caveolins serve as scaffolding proteins for the integration of signal transduction. Phosphorylation at Tyr14 is essential for caveolin association with SH2 or PTB domain-containing adaptor proteins, such as GRB7 (2-4).

Clathrin-coated vesicles provide for the intracellular transport of proteins following endocytosis and during multiple vesicle trafficking pathways. Vesicles form at specialized areas of the cell membrane where clathrin and associated proteins form clathrin-coated pits. Invagination of these cell membrane-associated pits internalizes proteins and forms an intracellular clathrin-coated vesicle (5,6). Clathrin is the most abundant protein in these vesicles and is present as a basic assembly unit called a triskelion. Each clathrin triskelion is composed of three clathrin heavy chains and three clathrin light chains. Clathrin heavy chain proteins are composed of several functional domains that associate with other vesicle proteins (6).

The APPL1 multidomain adaptor protein is a BAR-domain protein family member that is involved in membrane trafficking within a number of signal transduction pathways (7).

EEA1 is an early endosomal marker and a Rab5 effector protein essential for early endosomal membrane fusion and trafficking (8,9). Syntaxin 6 is a ubiquitously expressed S25C family member of the SNARE proteins (10,11). Syntaxin 6 protein is localized to the trans-Golgi and within endosomes and regulates membrane trafficking by partnering with a variety of other SNARE proteins (12-14). It has two coiled-coil domains (CC1 and CC2) located in the amino-terminal region and a PDZ domain in the carboxy-terminal region (15). The CC2 domain and its adjacent linker region mediate the association of GOPC with the Golgi protein golgin-160 and the Q-SNARE protein syntaxin 6 (15,16). The PDZ domain of GOPC interacts with the carboxy terminus of target proteins to mediate target protein vesicular trafficking and surface expression (17-20).

Rab5 is a member of the Ras superfamily of small Rab GTPases. Rab5 is localized at the plasma membrane and early endosomes and functions as a key regulator of vesicular trafficking during early endocytosis (21).

  1. Smart, E.J. et al. (1999) Mol Cell Biol 19, 7289-304.
  2. Nomura, R. and Fujimoto, T. (1999) Mol Biol Cell 10, 975-86.
  3. Volonté, D. et al. (2001) J Biol Chem 276, 8094-103.
  4. Lee, H. et al. (2000) Mol Endocrinol 14, 1750-75.
  5. Rodriguez-Boulan, E. et al. (2005) Nat Rev Mol Cell Biol 6, 233-47.
  6. Mousavi, S.A. et al. (2004) Biochem J 377, 1-16.
  7. Habermann, B. (2004) EMBO Rep 5, 250-5.
  8. Mu, F.T. et al. (1995) J Biol Chem 270, 13503-11.
  9. Christoforidis, S. et al. (1999) Nature 397, 621-5.
  10. Bock, J.B. et al. (2001) Nature 409, 839-41.
  11. Bock, J.B. et al. (1996) J Biol Chem 271, 17961-5.
  12. Wendler, F. and Tooze, S. (2001) Traffic 2, 606-11.
  13. Bock, J.B. et al. (1997) Mol Biol Cell 8, 1261-71.
  14. Mallard, F. et al. (2002) J Cell Biol 156, 653-64.
  15. Charest, A. et al. (2001) J Biol Chem 276, 29456-65.
  16. Hicks, S.W. and Machamer, C.E. (2005) J Biol Chem 280, 28944-51.
  17. Cheng, J. et al. (2002) J Biol Chem 277, 3520-9.
  18. He, J. et al. (2004) J Biol Chem 279, 50190-6.
  19. Wente, W. et al. (2005) J Biol Chem 280, 32419-25.
  20. Ito, H. et al. (2006) Biochem J 397, 389-98.
  21. Zerial, M. and McBride, H. (2001) Nat Rev Mol Cell Biol 2, 107-17.

Background References

    Trademarks and Patents

    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.
    All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

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    Orders: 877-616-CELL (2355)•[email protected]• Support: 877-678-TECH (8324)•[email protected]• Web: cellsignal.com
    For Research Use Only. Not for Use in Diagnostic Procedures.