Product Includes | Product # | Quantity | Mol. Wt | Isotype/Source |
---|---|---|---|---|
G9a/EHMT2 (C6H3) Rabbit mAb | 3306 | 20 µl | 160,180 kDa | Rabbit IgG |
ESET (C1C12) Rabbit mAb | 2196 | 20 µl | 180 kDa | Rabbit IgG |
ASH2L (D93F6) XP® Rabbit mAb | 5019 | 20 µl | 80, 65 kDa | Rabbit IgG |
RBBP5 (D3I6P) Rabbit mAb | 13171 | 20 µl | 70 kDa | Rabbit IgG |
SET7/SET9 Antibody | 2813 | 20 µl | 48 kDa | Rabbit  |
SMYD2 (D14H7) Rabbit mAb | 9734 | 20 µl | 49 kDa | Rabbit IgG |
SUV39H1 (D11B6) Rabbit mAb | 8729 | 20 µl | 48 kDa | Rabbit IgG |
SET8 (C18B7) Rabbit mAb | 2996 | 20 µl | 43 kDa | Rabbit IgG |
Anti-rabbit IgG, HRP-linked Antibody | 7074 | 100 µl | Goat  |
Please visit cellsignal.com for individual component applications, species cross-reactivity, dilutions, protocols, and additional product information.
Description
The Lysine Methyltransferase Antibody Sampler Kit provides a fast and economical means to evaluate endogenous levels of lysine methyltransferases. The kit contains enough primary antibody to perform two western blot experiments per primary antibody.
Storage
Background
SET domain-containing proteins are potential histone methyltransferases (HMTases), which are classified into subgroups by their putative substrate specificities. Histone H3 Lys9 (H3-K9) methyltransferase group genes include Suv39h1, Suv39h2, G9a, G9a related protein (GLP) and SETDB1/ESET (1). The H3-K9 methylation mark plays an important role as a binding site for the chromo-containing protein, resulting in chromatin compaction and heterochromatin generation (2). Histone H3-K4 methylation is exclusively associated with actively transcribed genes (2). The first H3-K4 methylase complex, COMPASS, was identified in the yeast S. cerevisiae and consists of Set1/KMT2 and seven other polypeptides, Cps60-Cps15 (2). Set1/KMT2 functions within COMPASS and is capable of mono-, di-, and trimethylating H3-K4 (2). There are several Set1 related proteins in mammals including WDR5, RBBP5, ASH2L, CXXC1, and DPY30 (2,3). SET7/SET9 is a member of the SET domain-containing family that can specifically methylate H3-K4, Lys189 of the TAF10, a member of the TFIID transcription factor complex, and Lys372 of the p53 tumor suppressor protein (4-6). SET domain-containing lysine methyltransferase 8 (SET8), also known as PR/SET domain-containing protein 7 (PR/SET7), is a single-subunit enzyme that mono-methylates histone H4-K20, preferably on nucleosomal substrates (7-9). SET and MYND domain-containing protein 2 (SMYD2), also known as lysine methyltransferase protein 3C (KMT3C), functions to repress transcription by interacting with the Sin3A repressor complex and methylating H3-K36 (10). SMYD2 also methylates H3-K4 through interaction with HSP90α, and methylates p53 at Lys370 to repress p53-mediated transcriptional activation and apoptosis (11,12).
- Tachibana, M. et al. (2005) Genes Dev 19, 815-26.
- Shilatifard, A. (2008) Curr Opin Cell Biol 20, 341-8.
- Lee, J.H. et al. (2007) J Biol Chem 282, 13419-28.
- Nishioka, K. et al. (2002) Genes Dev 16, 479-89.
- Kouskouti, A. et al. (2004) Mol Cell 14, 175-82.
- Chuikov, S. et al. (2004) Nature 432, 353-60.
- Fang, J. et al. (2002) Curr Biol 12, 1086-99.
- Xiao, B. et al. (2005) Genes Dev 19, 1444-54.
- Couture, J.F. et al. (2005) Genes Dev 19, 1455-65.
- Brown, M.A. et al. (2006) Mol Cancer 5, 26.
- Abu-Farha, M. et al. (2008) Mol Cell Proteomics 7, 560-72.
- Huang, J. et al. (2006) Nature 444, 629-32.
Background References
Trademarks and Patents
限制使用
除非 CST 的合法授书代表以书面形式书行明确同意,否书以下条款适用于 CST、其关书方或分书商提供的书品。 任何书充本条款或与本条款不同的客书条款和条件,除非书 CST 的合法授书代表以书面形式书独接受, 否书均被拒书,并且无效。
专品专有“专供研究使用”的专专或专似的专专声明, 且未专得美国食品和专品管理局或其他外国或国内专管机专专专任何用途的批准、准专或专可。客专不得将任何专品用于任何专断或治专目的, 或以任何不符合专专声明的方式使用专品。CST 专售或专可的专品提供专作专最专用专的客专,且专用于研专用途。将专品用于专断、专防或治专目的, 或专专售(专独或作专专成)或其他商专目的而专专专品,均需要 CST 的专独专可。客专:(a) 不得专独或与其他材料专合向任何第三方出售、专可、 出借、捐专或以其他方式专专或提供任何专品,或使用专品制造任何商专专品,(b) 不得复制、修改、逆向工程、反专专、 反专专专品或以其他方式专专专专专品的基专专专或技专,或使用专品开专任何与 CST 的专品或服专专争的专品或服专, (c) 不得更改或专除专品上的任何商专、商品名称、徽专、专利或版专声明或专专,(d) 只能根据 CST 的专品专售条款和任何适用文档使用专品, (e) 专遵守客专与专品一起使用的任何第三方专品或服专的任何专可、服专条款或专似专专