| Product Includes | Product # | Quantity | Mol. Wt | Isotype/Source |
|---|---|---|---|---|
| TBK1/NAK (E8I3G) Rabbit mAb | 38066 | 20 µl | 84 kDa | Rabbit IgG |
| MAVS (D5A9E) Rabbit mAb | 24930 | 20 µl | 75, 52 kDa | Rabbit IgG |
| TRAF2 (C192) Antibody | 4724 | 20 µl | 53 kDa | Rabbit |
| TRAF3 (E8H3B) Rabbit mAb | 33640 | 20 µl | 62 kDa | Rabbit IgG |
| TRAF6 (E2K9D) Rabbit mAb | 67591 | 20 µl | 60 kDa | Rabbit IgG |
| Phospho-TBK1/NAK (Ser172) (D52C2) XP® Rabbit mAb | 5483 | 20 µl | 84 kDa | Rabbit IgG |
| IRF-3 (D6I4C) XP® Rabbit mAb | 11904 | 20 µl | 50-55 kDa | Rabbit IgG |
| Phospho-IRF-3 (Ser386) (E7J8G) XP® Rabbit mAb | 37829 | 20 µl | 50-55 kDa | Rabbit IgG |
| Phospho-IRF-3 (Ser396) (D6O1M) Rabbit mAb | 29047 | 20 µl | 45-55 kDa | Rabbit IgG |
| Anti-rabbit IgG, HRP-linked Antibody | 7074 | 100 µl | Goat |
Please visit cellsignal.com for individual component applications, species cross-reactivity, dilutions, protocols, and additional product information.
Description
The MAVS Signalosome Antibody Sampler Kit provides an economical means of detecting the activation of signaling pathways in which MAVS plays a critical role. The kit includes enough antibodies to perform two western blot experiments with each primary antibody.
Storage
Background
Recognition of conserved molecular structures of viruses by host pattern-recognition receptors (PRRs) initiates innate antiviral immune responses. Several families of PRRs have been demonstrated to sense different microbial components (1,2). The RIG-I-like receptor (RLR) family, including RIG-I, MDA5, and LGP2, are the primary PRRs to recognize viral RNAs (3,4). Upon binding to viral RNA, these receptors undergo conformation change, leading to their interaction with mitochondrial antiviral signaling protein (MAVS). MAVS subsequently forms large prion-like polymers and serves as a platform to recruit multiple components, including TRAF proteins and TBK1, to form the so-called MAVS signalosome. MAVS signalosome, in turn, activates the IRF-3 and NF-kB pathways, leading to the production of type I IFNs and pro-inflammatory cytokines (5-9).
- Akira, S. et al. (2006) Cell 124, 783-801.
- Kumar, H. et al. (2011) Int Rev Immunol 30, 16-34.
- Nakhaei, P. et al. (2009) Semin Immunol 21, 215-22.
- Yoneyama, M. et al. (2015) Curr Opin Immunol 32, 48-53.
- Seth, R.B. et al. (2005) Cell 122, 669-82.
- Vazquez, C. and Horner, S.M. (2015) J Virol 89, 6974-7.
- Reikine, S. et al. (2014) Front Immunol 5, 342.
- Dutta, S. et al. (2020) Front Microbiol 11, 1990.
- Thoresen, D. et al. (2021) Immunol Rev 304, 154-168.
Background References
Trademarks and Patents
限制使用
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