| Product Includes | Product # | Quantity | Mol. Wt | Isotype/Source |
|---|---|---|---|---|
| STING (D1V5L) Rabbit mAb | 50494 | 20 µl | 33, 35 kDa | Rabbit IgG |
| Phospho-STING (Ser365) (D8F4W) Rabbit mAb | 72971 | 20 µl | 41 kDa | Rabbit IgG |
| cGAS (D3O8O) Rabbit mAb | 31659 | 20 µl | 62 kDa | Rabbit IgG |
| TBK1/NAK (D1B4) Rabbit mAb | 3504 | 20 µl | 84 kDa | Rabbit IgG |
| Phospho-TBK1/NAK (Ser172) (D52C2) XP® Rabbit mAb | 5483 | 20 µl | 84 kDa | Rabbit IgG |
| IRF-3 (D83B9) Rabbit mAb | 4302 | 20 µl | 45-55 kDa | Rabbit IgG |
| Phospho-IRF-3 (Ser396) (D6O1M) Rabbit mAb | 29047 | 20 µl | 45-55 kDa | Rabbit IgG |
| Anti-rabbit IgG, HRP-linked Antibody | 7074 | 100 µl | Goat |
Please visit cellsignal.com for individual component applications, species cross-reactivity, dilutions, protocols, and additional product information.
Description
The Mouse-Reactive STING Pathway Antibody Sampler Kit provides an economical means of detecting activation and expression of key components of the STING pathway using phospho-specific and control antibodies. The kit includes enough antibody to perform two western blot experiments with each primary antibody.
Storage
Background
Stimulator of interferon genes (STING, TMEM173, MITA) is a transmembrane adaptor protein that is a critical component of the cellular innate immune response to pathogenic cytoplasmic DNA (1,2). STING is a ubiquitously expressed protein found predominantly in the ER (1). The enzyme cGAMP synthase (cGAS) produces the second messenger cyclic-GMP-AMP (cGAMP) in response to cytoplasmic DNA (3,4). cGAMP binds and activates STING (3,4). In addition, detection of cytoplasmic DNA by nucleic acid sensors, including DDX41 or IFI16, results in STING activation (5,6). Following activation, STING translocates with TBK1 to perinuclear endosomes and gets phosphorylated by ULK1 at Ser366 (Ser365 in mouse) (7,8). The TBK1 kinase phosphorylates and activates IRF-3 and NF-κB, which leads to the induction of type I interferon and other immune response genes (1,2,7).
- Ishikawa, H. and Barber, G.N. (2008) Nature 455, 674-8.
- Zhong, B. et al. (2008) Immunity 29, 538-50.
- Sun, L. et al. (2013) Science 339, 786-91.
- Wu, J. et al. (2013) Science 339, 826-30.
- Zhang, Z. et al. (2011) Nat Immunol 12, 959-65.
- Unterholzner, L. et al. (2010) Nat Immunol 11, 997-1004.
- Ishikawa, H. et al. (2009) Nature 461, 788-92.
- Konno, H. et al. (2013) Cell 155, 688-98.
Background References
Trademarks and Patents
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