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细胞器标记物

对蛋白亚细胞定位的认知可能表明,蛋白可能在许多细胞进程中发挥作用。如果与下面所列的其中一种细胞器特异性试剂共定位,那么可确认目的蛋白的亚细胞位置。虽然这些细胞器标记物可用作强大的免疫荧光工具,但它们还可用作碎裂细胞裂解物的蛋白质印迹对照。

这些蛋白的表达可能在不同细胞和组织中有所不同。

细胞骨架

编号 名称 应用 反应性
8844 β-Actin (13E5) Rabbit mAb (Alexa Fluor® 488 Conjugate) IF-IC, F H, M, R, Mk, B, Pg, (Hm, C, Dg, Hr)
9470 β-Actin (13E5) Rabbit mAb (Alexa Fluor® 594 Conjugate) IF-IC H, M, R, Mk, B, Pg, (C, Dg, Hr)
8456 Pan-Actin (D18C11) Rabbit mAb W, IHC-P, IF-IC H、M、R、Mk
8584 β-Actin (13E5) Rabbit mAb (Alexa Fluor® 647 Conjugate) IF-IC, F H, M, R, Mk, B, Pg, (C, Dg, Hr)
8457 β-Actin (D6A8) Rabbit mAb W, IF-IC, F H、M、R、Mk
4970 β-Actin (13E5) Rabbit mAb W, IHC-P, IHC-F, IF-IC, F H, M, R, Mk, B, Pg, (Hm, C, Dg, Hr)
3700 β-Actin (8H10D10) Mouse mAb W, IHC-P, IF-IC, F H, M, R, Hm, Mk, Dg
5332 Desmin (D93F5) XP® Rabbit mAb W, IF-F, IF-IC H, M, R, (Mk)
13063 Keratin 20 (D9Z1Z) XP® Rabbit mAb W, IHC-P, IF-IC, F H
4528 Pan-Keratin (C11) Mouse mAb (Alexa Fluor® 647 Conjugate) IF-P, IF-IC, F H, R, Mk
4523 Pan-Keratin (C11) Mouse mAb (Alexa Fluor® 488 Conjugate) IF-P, IF-IC, F H, R, Mk
4545 Pan-Keratin (C11) Mouse mAb W, IHC-P, IF-P, IF-IC, F H, R, Mk
4898 Keratin 7 (R458) Antibody W, IF-IC H
4543 Keratin 17 (D73C7) XP® Rabbit mAb W, IF-IC, F H, M, R, (Mk, Dg)
12434 Keratin 17/19 (D4G2) XP® Rabbit mAb W, IHC-P, IF-IC H, M, R
8953 Alexa Fluor® 555 Phalloidin IF-F, IF-IC 全部
12956 DyLight™ 650 Phalloidin IF-F, IF-IC 全部
12877 DyLight™ 594 Phalloidin IF-F, IF-IC 全部
13054 DyLight™ 554 Phalloidin IF-F, IF-IC 全部
12935 DyLight™ 488 Phalloidin IF-F, IF-IC 全部
12848 DyLight™ 350 Phalloidin IF-F, IF-IC 全部
8940 Alexa Fluor® 647 Phalloidin IF-F, IF-IC 全部
8878 Alexa Fluor® 488 Phalloidin IF-F, IF-IC 全部
3623 β-Tubulin (9F3) Rabbit mAb (Alexa Fluor® 488 Conjugate) IF-IC, F H, M, R, Mk, Dm, Z, B, (C)
3624 β-Tubulin (9F3) Rabbit mAb (Alexa Fluor® 647 Conjugate) IF-IC, F H, M, R, Mk, Dm, Z, B, (C)
2116 β-Tubulin (9F3) Rabbit mAb (Alexa Fluor® 555 Conjugate) IF-IC H, M, Mk, Dm, Z, B, (C)
2128 β-Tubulin (9F3) Rabbit mAb W, IHC-P, IF-IC, F H, M, R, Mk, Z, B, (C)
2146 β-Tubulin Antibody W, IHC-P, IF-IC, F H, M, R, Mk, Z, B, (X)
2148 α/β-Tubulin Antibody W, IHC-P, IF-IC, F H, M, R, Mk, Z, B
2125 α-Tubulin (11H10) Rabbit mAb W, IHC-P, IF-IC, F H, M, R, Mk, Dm, B, (Dg, Pg)
3873 α-Tubulin (DM1A) Mouse mAb W, IHC-P, IF-IC H、M、R、Mk
2144 α-Tubulin Antibody W, IHC-P, IF-IC, F H, M, R, Mk, Dm, B, (X)
5741 Vimentin (D21H3) XP® Rabbit mAb W, IHC-P, IF-IC, F H、M、R、Mk

细胞浆

编号 名称 应用 反应性
8727 MEK1/2 (D1A5) Rabbit mAb W, IF-IC, F H, M, R, Mk, (Hm), Dm, (X, Z, B, Dg, Pg, Ce)
4694 MEK1/2 (L38C12) Mouse mAb W, IHC-P, IF-IC, F H、M、R、Mk

中心粒 / 中心体

编号 名称 应用 反应性
5259 PCM-1 (Q15) Antibody W, IP, IF-IC, F H, (Mk)
5213 PCM-1 (G2000) Antibody W, IP, IF-IC, F H, M, (Mk)

核糖体

编号 名称 应用 反应性
2415 Ribosomal Protein L7a (E109) Antibody W, IF-IC H、M、R、Mk
9538 Ribosomal Protein S3 (D50G7) XP® Rabbit mAb W, IP, IF-IC H、M、R、Mk
2579 Ribosomal Protein S3 Antibody W, IF-IC H、M、R、Mk
2217 S6 Ribosomal Protein (5G10) Rabbit mAb W, IHC-P, IF-F, IF-IC H, M, R, Mk, (Pg)
6989 S6 Ribosomal Protein (54D2) Mouse mAb (Alexa Fluor® 555 Conjugate) IF-IC H, M, R, Mk, Dm
5317 S6 Ribosomal Protein (54D2) Mouse mAb (Alexa Fluor® 488 Conjugate) IF-F, IF-IC, F H, M, R, Dm
2317 S6 Ribosomal Protein (54D2) Mouse mAb W, IP, IHC-P, IF-IC, F H, M, R, Mk, Dm
5548 S6 Ribosomal Protein (54D2) Mouse mAb (Alexa Fluor® 647 Conjugate) IF-F, IF-IC, F H, M, R, Mk, Dm

内质网

编号 名称 应用 反应性
8787 ER-Tracker™ Green (BODIPY® FL Glibenclamide) IF-IC  
5033 ERp72 (D70D12) XP® Rabbit mAb W, IF-IC, F H、M、R、Mk
3501 PDI (C81H6) Rabbit mAb W, IHC-P, IF-IC H、M、R、Mk

高尔基体

编号 名称 应用 反应性
12480 GM130 (D6B1) XP® Rabbit mAb W, IP, IF-IC H、Mk
12290 RCAS1 (D2B6N) XP® Rabbit mAb W, IP, IF-IC, F H, M, R, (Mk)
13192 Golgin-97 (D8P2K) Rabbit mAb W, IP, IF-IC H, M

线粒体

编号 名称 应用 反应性
5318 AIF (D39D2) XP® Rabbit mAb W, IP, IF-IC H, M, R, Mk, (B, Dg)
7561 COX IV (3E11) Rabbit mAb (Alexa Fluor® 647 Conjugate) IF-IC, F H, R, Mk, Z, B, Pg
8693 COX IV (3E11) Rabbit mAb (Alexa Fluor® 555 Conjugate) IF-IC H, R, Mk, Z, B, Pg
4853 COX IV (3E11) Rabbit mAb (Alexa Fluor® 488 Conjugate) IF-IC, F H, R, Mk, Z, B, Pg
8692 COX IV (3E11) Rabbit mAb (Alexa Fluor® 594 Conjugate) IF-IC H, R, Mk, Z, B, Pg
4850 COX IV (3E11) Rabbit mAb W, IP, IHC-P, IHC-F, IF-IC, F H, R, Mk, Z, B, Pg
4844 COX IV Antibody W, IP, IHC-P, F H, M, R, Mk, B
12963 Cytochrome c (6H2.B4) Mouse mAb IP, IF-IC H, M, R
2024 Hexokinase I (C35C4) Rabbit mAb W, IP, IHC-P, IF-IC H, M

内体

编号 名称 应用 反应性
3267 Caveolin-1 (D46G3) XP® Rabbit mAb W, IP, IHC-P, IF-IC, F H, M, R, Hm, Mk, B, Dg
3238 Caveolin-1 Antibody W, IP, IHC-P, IHC-F, IF-IC, F H, M, R, Hm, Z, B, Pg
8522 Caveolin-2 (D4A6) XP® Rabbit mAb W, IP, IF-IC H、Mk
4796 Clathrin Heavy Chain (D3C6) XP® Rabbit mAb W, IP, IF-IC H, M, R, Mk, (Pg)
2410 Clathrin Heavy Chain (P1663) Antibody W, IF-IC H, M, R, Mk, B, (Pg)
3288 EEA1 (C45B10) Rabbit mAb W, IP, IF-IC H, M, R
2411 EEA1 Antibody W, IP, IF-IC H、M、R、Mk
5589 Rab11 (D4F5) XP® Rabbit mAb W, IP, IF-IC H, M, R, Mk, (Hm, Pg)
3547 Rab5 (C8B1) Rabbit mAb W, IF-IC H、M、R、Mk
2143 Rab5 Antibody W, IF-IC H, M, R, Hm, Mk
9367 Rab7 (D95F2) XP® Rabbit mAb W, IP, IF-IC H、M、R、Mk
5118 Rab9 (D52G8) XP® Rabbit mAb W, IP, IF-IC H、M、R、Mk

自噬小体

编号 名称 应用 反应性
2011 Atg12 Antibody (Mouse Specific) W, IP, IF-IC M
2010 Atg12 Antibody (Human Specific) W, IP, IF-IC H
4599 LC3A (D50G8) XP® Rabbit mAb W, IHC-P, IF-IC, F H, M, R, (Mk, Dg)
13118 LC3A/B (D3U4C) XP® Rabbit mAb (Biotinylated) W H, M, R, (Mk, X, B, Dg, Pg)
13082 LC3A/B (D3U4C) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate) IF-IC, F H, M, R, (Mk, X, B, Dg, Pg)
12741 LC3A/B (D3U4C) XP® Rabbit mAb W, IHC-P, IF-IC, F H, M, R, (Mk, X, B, Dg, Pg)
13173 LC3A/B (D3U4C) XP® Rabbit mAb (Alexa Fluor® 555 Conjugate) IF-IC H, M, R, (Mk, X, B, Dg, Pg)
13611 LC3A/B (D3U4C) XP® Rabbit mAb (PE Conjugate) F H, M, R, (X, B, Dg, Pg)
3868 LC3B (D11) XP® Rabbit mAb W, IP, IHC-P, IF-IC, F H, M, R, (Mk, B, Pg)
2775 LC3B Antibody W, IF-IC, F H, M, R, (Mk, B, Pg)
7695 SQSTM1/p62 (D10E10) Rabbit mAb (IF Preferred) IP, IF-IC H

溶酶体

编号 名称 应用 反应性
9091 LAMP1 (D2D11) XP® Rabbit mAb W, IP, IF-IC H、Mk

胞核

编号 名称 应用 反应性
5019 ASH2L (D93F6) XP® Rabbit mAb W, IP, IF-IC H, M, R, Mk, (Dm)
2196 ESET (C1C12) Rabbit mAb W, IP, IF-IC H、Mk
2718 Histone H2A.Z Antibody W, IP, IF-IC H, M, R, Mk, Z, (C, X, B)
2184 LSD1 (C69G12) Rabbit mAb W, IP, IHC-P, IHC-F, IF-IC H、M、R、Mk
2139 LSD1 Antibody W, IP, IHC-P, IF-IC, F H、M、R、Mk

核被膜

编号 名称 应用 反应性
4777 Lamin A/C (4C11) Mouse mAb W、IP、IHC-P、IF-F、IF-IC、F H、M、R、Mk
12255 Lamin B2 (D8P3U) Rabbit mAb W, IP, IF-IC H、Mk
2598 NUP98 (C39A3) Rabbit mAb W, IP, IF-IC H、M、R、Mk

胞核

编号 名称 应用 反应性
3833 eIF6 (D16E9) XP® Rabbit mAb W, IP, IF-IC H、M、R、Mk
2639 Fibrillarin (C13C3) Rabbit mAb W, IF-IC H、M、R、Mk

过氧物酶体

编号 名称 应用 反应性
12980 Catalase (D4P7B) XP® Rabbit mAb W, IP, IF-IC H

着丝粒

编号 名称 应用 反应性
2048 CENP-A (C51A7) Rabbit mAb (Mouse Specific; IF Preferred) W, IF-IC M
2186 CENP-A Antibody W, IF-IC H

细胞间连接

编号 名称 应用 反应性
3199 E-Cadherin (24E10) Rabbit mAb (Alexa Fluor® 488 Conjugate) IF-P, IF-IC, F H, M, (B, Dg, Pg)
3195 E-Cadherin (24E10) Rabbit mAb W, IHC-P, IHC-F, IF-IC, F H, M, (B, Dg, Pg)
7687 E-Cadherin (24E10) Rabbit mAb (Alexa Fluor® 594 Conjugate) IF-IC H, M, (Dg, Pg)
4295 E-Cadherin (24E10) Rabbit mAb (Alexa Fluor® 555 Conjugate) IF-IC H, M, (Dg)
13116 N-Cadherin (D4R1H) XP® Rabbit mAb W, IP, IHC-P, IF-IC H
2189 P-Cadherin (C13F9) Rabbit mAb W, IF-IC H, (Mk)
2500 VE-Cadherin (D87F2) XP® Rabbit mAb W, IP, IF-IC, F H, Dm, B, Pg, (Mk)
3512 Connexin 43 Antibody W, IHC-P, IHC-F, IF-F, IF-IC H, M, R, Mk, Z, (Dg, Pg)
2847 ZO-2 Antibody W, IF-IC H, M, R, Mk, B, Dg
3704 ZO-3 (D57G7) XP® Rabbit mAb W, IF-IC H

应用缩写: W = 蛋白质印迹法 IP = 免疫沉淀法 IHC-P = 免疫组织化学法(石蜡) IHC-F = 免疫组织化学法(冷冻) IF-IC = 免疫荧光法(免疫细胞化学) IF-F = 免疫荧光法(冷冻) IF-P = 免疫荧光法(石蜡) F = 流式细胞术 
反应性缩写: H = 人 M = 小鼠 R = 大鼠 Hm = 仓鼠 Mk = 猴 C = 鸡 Dm = 果蝇 X =非洲爪蟾 Z = 斑马鱼 B = 牛 Dg = 狗 Pg = 猪 Hr = 马 Ce = 秀丽隐杆线虫 All = 预计所有物种 
根据 100% 序列同源性,括号里的物种预测有反应。

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