PTMScan Discovery

PTMScan® 基序抗体试剂盒是一条可应用 PTMScan 技术(Cell Signaling Technology (CST) 开发的一项专利技术)的产品线。本试剂盒提供我们​精选的拥有专利的基序抗体,可配合 PTMScan 技术在研究中使用。这些抗体与 Cell Signaling Technology 的科学家在 PTMScan Discovery Services 中使用的完全一致。对于具备质谱蛋白质组学内行专业知识的客户来说,这些试剂盒提供了一种高性价比的方式,使其可以利用任意 CST™ Motif IAP Beads,及有限使用许可​使用 CST PTMScan 技术。每个试剂盒中都提供了 10 测定所需的试剂和详细的实验步骤。

为什么在研究磷酸化作用时要考虑抗体富集?

  • 基于氨基酸 (S/T/Y) 或目的基序,聚焦发现磷酸化位点
  • 与 IMAC 相比,低发生率磷酸化事件覆盖面增加
  • 观察数据,显示抗体富集法与 IMAC 之间的互补性

PTMScan® 基序抗体试剂盒

编号 PTMScan® Kit Description 推荐的蛋白酶处理方法 修饰类型 LCMS 数据和结果导出
No. PTMScan® Kit Description Recommended Protease Treatment Modification Type LCMS Data & Output Results
13416 PTMScan® Acetyl-Lysine Motif [Ac-K] Kit Trypsin Acetylation Mouse Liver
XLS  |  RAW
14499 PTMScan® Pilot Acetyl-Lysine Motif [Ac-K] Kit Trypsin Acetylation Mouse Liver
XLS  |  RAW
5561 PTMScan® Phospho-Akt Substrate Motif mAb 1 (RXXS*/T*) Kit LysC* Phosphorylation Mouse Liver
XLS  |  RAW
5563 PTMScan® Phospho-Akt Substrate Motif mAb 2 (RXRXXS*/T*) Kit LysC* Phosphorylation Mouse Liver
XLS  |  RAW
5564 PTMScan® Phospho-AMPK Substrate Motif (LXRXXS*/T*) Kit LysC* Phosphorylation Mouse Liver
XLS  |  RAW
12267 PTMScan® Phospho-ATM/ATR Substrate Motif [pSQ] Kit Trypsin Phosphorylation Mouse Liver
XLS  |  RAW
12235 PTMScan® Mono-Methyl Arginine Motif [mme-RG] Kit Trypsin* Methylation Mouse Embryo
XLS  |  RAW
13474 PTMScan® Asymmetric Di-Methyl Arginine Motif [adme-R] Kit Trypsin* Methylation Mouse Embryo
XLS  |  RAW
13563 PTMScan® Symmetric Di-Methyl Arginine Motif [sdme-RG] Kit Trypsin* Methylation Mouse Embryo
XLS  |  RAW
12810 PTMScan® Cleaved Caspase Substrate Motif [DE(T/S/A)D] Kit Trypsin Cleaved Mouse Liver
XLS  |  RAW
12170 PTMScan® Phospho-CK2 Substrate Motif (S*/T*DXE) Kit Trypsin Phosphorylation Mouse Liver
XLS  |  RAW
14809 PTMScan® Pan-Methyl Lysine Kit Trypsin* Methylation Mouse Liver
XLS  |  RAW
14990 PTMScan® Phospho-MAPK Substrate Motif [PXpTP] Kit Trypsin Phosphorylation Hela Mitotic
XLS  |  RAW
4652 PTMScan® Phospho-MAPK/CDK Substrate Motif (PXS*P and S*PXK/R) Kit Trypsin Phosphorylation Mouse Liver
XLS  |  RAW
5565 PTMScan® Phospho-PKA Substrate Motif (RRXS*/T*) Kit LysC* Phosphorylation Mouse Liver
XLS  |  RAW
14989 PTMScan® Phospho-Ser-Pro-Pro Motif [pSPP] Kit Trypsin Phosphorylation Hela Mitotic
XLS  |  RAW
5636 PTMScan® Phospho-Tyrosine Mouse mAb (P-Tyr-100) Kit Trypsin Phosphorylation Mouse Brain
XLS  |  RAW
8803 PTMScan® Phospho-Tyrosine Rabbit mAb (P-Tyr-1000) Kit Trypsin Phosphorylation Mouse Brain
XLS  |  RAW
14478 PTMScan® Pilot Phospho-Tyrosine Rabbit mAb (P-Tyr-1000) Trypsin Phosphorylation Mouse Brain
XLS  |  RAW
5567 PTMScan® Phospho-T*PP Motif (T*PP) XP® Kit Trypsin Phosphorylation Mouse Liver
XLS  |  RAW
5566 PTMScan® Phospho-ST*P Motif (ST*P) XP® Kit Trypsin Phosphorylation Mouse Liver
XLS  |  RAW
13764 PTMScan® Succinyl-Lysine Motif [Succ-K] Kit Trypsin Succinylation Mouse Liver
XLS  |  RAW
5562 PTMScan® Ubiquitin Remnant Motif (K-ε-GG) Kit Trypsin Ubiquitin Mouse Liver
XLS  |  RAW
14482 PTMScan® Pilot Ubiquitin Remnant Motif (K-ε-GG) Kit Trypsin Ubiquitin Mouse Liver
XLS  |  RAW
32948 PTMScan® Basophilic Kinase Substrate Motif [(R/X)(R/X)Xp(S/T)] Kit Lys C Phosphorylation XLS
28303 PTMScan® Phospho-Ser/Thr-Pro Motif [p(S/T)P] Kit Trypsin Phosphorylation XLS
25081 PTMScan® Phospho-Ser/Thr Motif [pS/T] Kit Lys C Phosphorylation XLS
17848 PTMScan® Propionyl-Lysine [Prop-K] Kit Trypsin Propionylation Mouse Liver
XLS
26101 PTMScan® Glutaryl-Lysine [Glut-K] Kit Trypsin Glutarylation Mouse Liver
XLS
93872 PTMScan® Malonyl-Lysine [Mal-K] Kit Trypsin Malonylation Mouse Liver
XLS
10821 PTMScan® Phospho-PDK1 Docking Motif [(F/Y)p(S/T)(F/Y)] Kit Trypsin Phosphorylation Mouse Embryo
XLS
91336 PTMScan® Phospho-Thr-X-Arg Motif [pT(P/X)R] Kit Trypsin Phosphorylation HT29 Cells
XLS
71652 PTMScan® Phospho-PKC Substrate Motif [(R/K)(R/K)Xp(S/T)X(R/X)] Kit Lys C Phosphorylation Mouse Embryo
XLS
97673 PTMScan® Kinase Activation Antibody Sampler Kit Lys C/Trypsin Phosphorylation  
86506 PTMScan® Lysine/Arginine Modification Antibody Sampler Kit Trypsin Acetylation / Methylation  
65666 PTMScan® Cell Growth and Death Antibody Sampler Kit Lys C/Trypsin Phosphorylation  

* 建议 IP 后进行二次胰蛋白酶消化

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