Detailed technical support information to support ChIP experimental success. LInk to CST's comprehensive ChIP protocol.
Our ChIP assay kits avoid the problems of traditional sonication protocols, which require subjecting chromatin to harsh, denaturing conditions. Learn more.
Chromatin immunoprecipitation, or ChIP, is a technique that uses antibodies to isolate specific DNA-binding proteins along with the bound DNA fragments from cells and tissues.
Chromatin IP (ChIP) Frequently Asked Questions (FAQs) from Cell Signaling Technology, Inc.
The benefits of using micrococcal nuclease to digest chromatin compared to sonication.
SimpleChIP® Plus Sonication Chromatin Immunoprecipitation Protocol (Magnetic Beads): easy to follow directions describing the step by step experimental procedure
SimpleChIP Plus Chromatin Immunoprecipitation Protocol (Agarose Beads): easy to follow directions describing the step by step experimental procedure.a
The chromatin immunoprecipitation (ChIP) assay is a powerful and versatile technique to probe protein-DNA interactions within the natural chromatin context of the cell.
ChIP-seq is a powerful technique that combines ChIP and next-generation sequencing (NGS) to study DNA-protein interactions across the entire genome, but it is only as good as the quality of the antibody used in the ChIP experiment.
SimpleChIP Plus Chromatin Immunoprecipitation Protocol (Magnetic Beads): easy to follow directions describing the step by step experimental procedure.
Step-by-step ChIP assay protocol (agarose beads). A powerful technique used to study protein-DNA interactions, identify regulatory elements. Learn more.
Application protocols: easy to follow directions describing the step by step experimental procedure.
This is a four-part series featuring tips to improve your ChIP protocol.
SimpleChIP Chromatin Immunoprecipitation Protocol (Magnetic Beads): easy to follow directions describing the step by step experimental procedure.
In our experience, digesting 4x10^6 cells yields 10-20 ug of chromatin, which is why this is mentioned in our ChIP FAQ. However, as stated in the protocols for our SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003 and SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005, approximately 5ug - 10ug chromatin is required for ChIP depending on your target. In our experience, for histone (PTM) ChIP, as low as 1-2 ug of chromatin DNA is required per IP. For transcription factor ChIP, a minimum of 5-8 ug chromatin DNA is required per IP. For co-factor ChIP, a minimum of 10 ug chromatin is necessary per IP. This is why all of our validated antibodies have optimal dilutions based on 10ug per IP.
Use SimpleChIP kits to perform high throughput ChIP-Sequencing and assay genome wide changes in histone modifications and transcription factor binding.
Chromatin Immunoprecipitation Troubleshooting Guide: easy to follow directions describing the step by step experimental procedure.
Find out which epigenetic markers are important drivers in mixd lineage leukemia, or MLL.
Protein-DNA interactions mediate epigenetic processes. Researchers studying these interactions need a means of analyzing when & where they occur on the genome.
Chromatin Immunoprecipitation Workflow Solutions for ChIP-qPCR and ChIP-seq
Discover the key epigenetic drivers of breast cancer and the best products to target them.
Assay Kits from CST contain ChIP validated antibodies and control PCR primers to assay for pluripotency markers or epigenetic mark status in human or mouse samples.
Discover the most important epigenetic markers that contribute to the progression of diffuse intrinsic pontine glioma (DIPG).
Sonication ChIP Kit Performance Comparison
The success of your ChIP experiment depends on chromatin fragmentation. You could use sonication or enzymatic digestion. But how do you decide which?
Important factors to the ChIP assay include highly validated antibodies using enzyme chromatin digestion over sonication chromatin fragmentation
