Cell Signaling Technology

细胞器标记物

了解蛋白的亚细胞定位可以揭示其在许多细胞过程中的潜在功能。与下面列出来的细胞器特定的抗体共定位可以确定感兴趣蛋白质的亚细胞定位。当这些细胞器标记物在免疫荧光中发挥重要工具的作用同时,它们也可以用来作为分级的细胞裂解物的免疫印迹对照。

想要获得一份细胞器标记物的海报,请访问我们的资料索取部分 如果您想下载这个海报,请访问我们的市场资料部分

这些蛋白的表达在不同的细胞和组织中可能会有所不同。

小包装细胞器标记物抗体套装

Cellular Localization IF Antibody Sampler Kit #4753提供了一个通过免疫荧光-免疫细胞化学(IF-IC)来检测细胞器的经济方法。这个套装包括足够的抗体,可以进行最少20次IF-IC测试,每个抗体足够进行4次免疫印迹。

Atg12 Antibody (Human Specific) #2010 beta-Tubulin (9F3) Rabbit mAb #2128 Rab5 Antibody #2143 CENP-A Antibody #2186 Calnexin Antibody #2433 NUP98 (C39A3) Rabbit mAb #2598 Fibrillarin (C13C3) Rabbit mAb #2639 COX IV (3E11) Rabbit mAb #4850 Histone H3 Antibody #9715

Cellular Localization IF Antibody Sampler Kit #4753包含的抗体已经用IF-IC验证过了。这个小包装抗体套装可以满足以下细胞器的染色:自噬体,着丝粒,细胞骨架,内质网,内含体,线粒体,核膜,核仁和细胞核。

胞间连接

No. Name Applications Reactivity
7687 E-Cadherin (24E10) Rabbit mAb (Alexa Fluor® 594 Conjugate) IF-IC H, M, (Dg, Pg)
4295 E-Cadherin (24E10) Rabbit mAb (Alexa Fluor® 555 Conjugate) IF-IC H, M, (Dg)
3199 E-Cadherin (24E10) Rabbit mAb (Alexa Fluor® 488 Conjugate) IF-P, IF-IC, F H, M, (Dg)
3195 E-Cadherin (24E10) Rabbit mAb W, IHC-P, IHC-F, IF-IC, F H, M, (B, Dg, Pg)
2189 P-Cadherin (C13F9) Rabbit mAb W, IF-IC H, (Mk)
2130 P-Cadherin Antibody W H
2500 VE-Cadherin (D87F2) XP® Rabbit mAb W, IP, IF-IC, F H, Dm, B, Pg, (Mk)
2158 VE-Cadherin Antibody W, IF-IC H, Dm, B
3512 Connexin 43 Antibody W, IHC-P, IHC-F, IF-F, IF-IC H, M, R, Mk, Z, (Dg, Pg)
2847 ZO-2 Antibody W, IF-IC H, M, R, Mk, B, Dg
3704 ZO-3 (D57G7) XP® Rabbit mAb W, IF-IC H
 

细胞骨架

No. Name Applications Reactivity
9470 β-Actin (13E5) Rabbit mAb (Alexa Fluor® 594 Conjugate) IF-IC H, M, R, Mk, B, Pg, (C, Dg, Hr)
8457 β-Actin (D6A8) Rabbit mAb W, IF-IC, F H, M, R, Mk
8456 Pan-Actin (D18C11) Rabbit mAb W, IHC-P, IF-IC H, M, R, Mk
8844 β-Actin (13E5) Rabbit mAb (Alexa Fluor® 488 Conjugate) IF-IC, F H, M, R, Mk, B, Pg, (C, Dg, Hr)
4970 β-Actin (13E5) Rabbit mAb W, IHC-P, IHC-F, IF-IC, F H, M, R, Mk, B, Pg, (Hm, C, Dg, Hr)
8584 β-Actin (13E5) Rabbit mAb (Alexa Fluor® 647 Conjugate) IF-IC, F H, M, R, Mk, B, Pg, (C, Dg, Hr)
3700 β-Actin (8H10D10) Mouse mAb W, IHC-P, IF-IC, F H, M, R, Hm, Mk, Dg
5332 Desmin (D93F5) XP® Rabbit mAb W, IF-F, IF-IC H, M, R, (Mk)
4523 Pan-Keratin (C11) Mouse mAb (Alexa Fluor® 488 Conjugate) IF-P, IF-IC, F H, R, Mk
4528 Pan-Keratin (C11) Mouse mAb (Alexa Fluor® 647 Conjugate) IF-P, IF-IC, F H, R, Mk
4545 Pan-Keratin (C11) Mouse mAb W, IHC-P, IF-P, IF-IC, F H, R, Mk
4898 Keratin 7 (R458) Antibody W, IF-IC H
4543 Keratin 17 (D73C7) XP® Rabbit mAb W, IF-IC, F H, M, R, (Mk, Dg)
3624 β-Tubulin (9F3) Rabbit mAb (Alexa Fluor® 647 Conjugate) IF-IC, F H, M, R, Mk, Dm, Z, B, (C)
3623 β-Tubulin (9F3) Rabbit mAb (Alexa Fluor® 488 Conjugate) IF-IC, F H, M, R, Mk, Dm, Z, B, (C)
2128 β-Tubulin (9F3) Rabbit mAb W, IHC-P, IF-IC, F H, M, R, Mk, Z, B, (C)
2116 β-Tubulin (9F3) Rabbit mAb (Alexa Fluor® 555 Conjugate) IF-IC H, M, Mk, Dm, Z, B, (C)
2146 β-Tubulin Antibody W, IHC-P, IF-IC, F H, M, R, Mk, Z, B, (X)
2148 α/β-Tubulin Antibody W, IHC-P, IF-IC, F H, M, R, Mk, Z, B
2125 α-Tubulin (11H10) Rabbit mAb W, IHC-P, IF-IC, F H, M, R, Mk, Dm, B, (Dg, Pg)
3873 α-Tubulin (DM1A) Mouse mAb W, IHC-P, IF-IC H, M, R, Mk
2144 α-Tubulin Antibody W, IHC-P, IF-IC, F H, M, R, Mk, Dm, B, (X)
5741 Vimentin (D21H3) XP® Rabbit mAb W, IHC-P, IF-IC, F H, M, R, Mk
 

细胞质

No. Name Applications Reactivity
8727 MEK1/2 (D1A5) Rabbit mAb W, IF-IC, F H, M, R, Mk, (Hm), Dm, (X, Z, B, Dg, Pg, Ce)
4694 MEK1/2 (L38C12) Mouse mAb W, IHC-P, IF-IC, F H, M, R, Mk
 

核糖体

No. Name Applications Reactivity
2415 Ribosomal Protein L7a (E109) Antibody W, IF-IC H, M, R, Mk
9538 Ribosomal Protein S3 (D50G7) XP®Rabbit mAb W, IP, IF-IC H, M, R, Mk
2579 Ribosomal Protein S3 Antibody W, IF-IC H, M, R, Mk
2217 S6 Ribosomal Protein (5G10) Rabbit mAb W, IHC-P, IF-F, IF-IC H, M, R, Mk, (Pg)
2317 S6 Ribosomal Protein (54D2) Mouse mAb W, IP, IHC-P, IF-IC, F H, M, R, Mk, Dm
5317 S6 Ribosomal Protein (54D2) Mouse mAb (Alexa Fluor® 488 Conjugate) IF-F, IF-IC, F H, M, R, Dm
5548 S6 Ribosomal Protein (54D2) Mouse mAb (Alexa Fluor® 647 Conjugate) IF-F, IF-IC, F H, M, R, Mk, Dm
6989 S6 Ribosomal Protein (54D2) Mouse mAb (Alexa Fluor® 555 Conjugate) IF-IC H, M, R, Mk, Dm
 

内质网

No. Name Applications Reactivity
5033 ERp72 (D70D12) XP® Rabbit mAb W, IF-IC, F H, M, R, Mk
3501 PDI (C81H6) Rabbit mAb W, IHC-P, IF-IC H, M, R, Mk
2446 PDI Antibody W, IHC-P, IF-IC H, M, R, Mk
 

高尔基体

No. Name Applications Reactivity
12290 RCAS1 (D2B6N) XP® Rabbit mAb W, IP, IF-IC, F H, M, R, (Mk)
6960 RCAS1 Antibody W, IP, IF-IC, F H, M, R, Mk
 

线粒体

No. Name Applications Reactivity
5318 AIF (D39D2) XP® Rabbit mAb W, IP, IF-IC H, M, R, Mk, (B, Dg)
8693 COX IV (3E11) Rabbit mAb (Alexa Fluor® 555 Conjugate) IF-IC H, R, Mk, Z, B, Pg
8692 COX IV (3E11) Rabbit mAb (Alexa Fluor® 594 Conjugate) IF-IC H, R, Mk, Z, B, Pg
7561 COX IV (3E11) Rabbit mAb (Alexa Fluor® 647 Conjugate) IF-IC, F H, R, Mk, Z, B, Pg
4853 COX IV (3E11) Rabbit mAb (Alexa Fluor® 488 Conjugate) IF-F, IF-IC, F H, R, Mk, Z, B, Pg
4850 COX IV (3E11) Rabbit mAb W, IP, IHC-P, IHC-F, IF-IC, F H, R, Mk, Z, B, Pg
4844 COX IV Antibody W, IP, IHC-P, F H, M, R, Mk, B
2024 Hexokinase I (C35C4) Rabbit mAb W, IP, IHC-P, IF-IC H, M
 

内含体

No. Name Applications Reactivity
3267 Caveolin-1 (D46G3) XP®Rabbit mAb W, IP, IHC-P, IF-IC, F H, M, R, Hm, Mk, B, Dg
3238 Caveolin-1 Antibody W, IP, IHC-P, IHC-F, IF-IC, F H, M, R, Hm, Z, B, Pg
8522 Caveolin-2 (D4A6) XP® Rabbit mAb W, IP, IF-IC H, Mk
4796 Clathrin Heavy Chain (D3C6) XP® Rabbit mAb W, IP, IF-IC H, M, R, Mk, (Pg)
2410 Clathrin Heavy Chain (P1663) Antibody W, IF-IC H, M, R, Mk, B, (Pg)
3288 EEA1 (C45B10) Rabbit mAb W, IP, IF-IC H, M, R
2411 EEA1 Antibody W, IP, IF-IC H, M, R, Mk
5589 Rab11 (D4F5) XP® Rabbit mAb W, IP, IF-IC H, M, R, Mk, (Hm, Pg)
3547 Rab5 (C8B1) Rabbit mAb W, IF-IC H, M, R, Mk
2143 Rab5 Antibody W, IF-IC H, M, R, Hm, Mk
9367 Rab7 (D95F2) XP® Rabbit mAb W, IP, IF-IC H, M, R, Mk
5118 Rab9 (D52G8) XP® Rabbit mAb W, IP, IF-IC H, M, R, Mk
 

自噬体

No. Name Applications Reactivity
2010 Atg12 Antibody (Human Specific) W, IP, IF-IC H
2011 Atg12 Antibody (Mouse Specific) W, IP, IF-IC M
4599 LC3A (D50G8) XP® Rabbit mAb W, IHC-P, IF-IC, F H, M, R, (Mk, Dg)
3868 LC3B (D11) XP® Rabbit mAb W, IP, IHC-P, IF-IC, F H, M, R, (Mk, B, Pg)
2775 LC3B Antibody W, IF-IC, F H, M, R, (Mk, B, Pg)
7695 SQSTM1/p62 (D10E10) Rabbit mAb (IF Preferred) IP, IF-IC H
 

溶酶体

No. Name Applications Reactivity
9091 LAMP1 (D2D11) XP® Rabbit mAb W, IP, IF-IC H, Mk
 

细胞核

No. Name Applications Reactivity
2196 ESET (C1C12) Rabbit mAb W, IP, IF-IC H, Mk
2718 Histone H2A.Z Antibody W, IP, IF-IC H, M, R, Mk, Z, (C, X, B)
4499 Histone H3 (D1H2) XP®Rabbit mAb W, IHC-P, IF-IC H, M, R, Mk, (Hm, C, Dm, X, Z, B)
2184 LSD1 (C69G12) Rabbit mAb W, IP, IHC-P, IHC-F, IF-IC H, M, R, Mk
2139 LSD1 Antibody W, IP, IHC-P, IF-IC, F H, M, R, Mk
 

核膜

No. Name Applications Reactivity
4777 Lamin A/C (4C11) Mouse mAb W, IP, IHC-P, IF-F, IF-IC, F H, M, R, Mk
2598 NUP98 (C39A3) Rabbit mAb W, IP, IF-IC H, M, R, Mk
 

核仁

No. Name Applications Reactivity
5019 ASH2L (D93F6) XP® Rabbit mAb W, IP, IF-IC H, M, R, Mk, (Dm)
3833 eIF6 (D16E9) XP® Rabbit mAb W, IP, IF-IC H, M, R, Mk
2639 Fibrillarin (C13C3) Rabbit mAb W, IF-IC H, M, R, Mk
 

着丝粒

No. Name Applications Reactivity
2048 CENP-A (C51A7) Rabbit mAb (Mouse Specific; IF Preferred) W, IF-IC M
2186 CENP-A Antibody W, IF-IC H
5259 PCM-1 (Q15) Antibody W, IP, IF-IC, F H, (Mk)
5213 PCM-1 (G2000) Antibody W, IP, IF-IC, F H, M, (Mk)
 
 

应用:W=免疫印迹  IP=免疫沉淀  IHC-P=免疫组化(石蜡)  IHC-F=免疫组化(冰冻)  IF-IC=免疫荧光(免疫细胞化学)  IF-F=免疫荧光(冰冻) IF-P=免疫荧光(石蜡)F=流式细胞术

反应性:H=人  M=小鼠  R=大鼠Hm=仓鼠 Mk=猴  C=鸡  Dm=黑腹果蝇  X=非洲爪蟾 Z=斑马鱼  B=牛  Dg=狗 Pg=猪 Sc=酿酒酵母  Hr=马Ce=秀丽隐杆线虫

括号中的种属基于序列100%的同源性,预测可以反应。

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