Cell Signaling Technology

Alexa Fluor®偶联物

这些抗体与Alexa Fluor®488,555,594和647偶联,在流式和免疫荧光(IF-IC,IF-F)中使用。具有优秀的亮度与耐光性的Alexa Fluor®染料与来自CST的高质量抗体结合起来可以得到最强的信号而背景最低。所有的Alexa Fluor®偶联抗体都在特定应用中经过验证。

小包装偶联抗体试剂盒

No. Name
12046 Akt Alexa Fluor® 488 Conjugated Antibody Sampler Kit 

Kit includes 40 µl of the following conjugates:

12145 Apoptosis and Proliferation Alexa Fluor® 488 Conjugated Antibody Sampler Kit 

Kit includes 40 µl of the following conjugates:

12427 Epitope Tag Alexa Fluor® 488 Conjugated Antibody Sampler Kit 

Kit includes 40 µl of the following conjugates:

12207 MAPK Family Alexa Fluor® 647 Conjugated Antibody Sampler Kit 

Kit includes 40 µl of the following conjugates:

12348 Stat Family Alexa Fluor® 488 Conjugated Antibody Sampler Kit 

Kit includes 40 µl of the following conjugates:

细胞染料

No. Unconjugated Antibody Alexa 488 Alexa 555 Alexa 594 Alexa 647
  Phalloidin 8878 8953   8940

辅助产品

No. Unconjugated Antibody Alexa 488 Alexa 555 Alexa 594 Alexa 647
  Mouse IgG1 Isotype Control (MOPC-21) 4878     4843
5415 Mouse (G3A1) mAb IgG1 Isotype Control   9641 8527  
3900 Rabbit (DA1E) mAb IgG XP® Isotype Control 2975 3969 8760 2985
  Rabbit IgG (Polyclonal) Isotype Control 4340     3452

表位标签抗体

No. Unconjugated Antibody Reactivity Alexa 488 Alexa 555 Alexa 594 Alexa 647
2368 DYKDDDDK Tag Antibody (Binds to same epitope as Sigma's Anti-FLAG® M2 Antibody) All 5407 3768 9696 3916
2367 HA-Tag (6E2) Mouse mAb All 2350     3444
2624 GST (26H1) Mouse mAb All 3368 3720 9440 3445
2276 Myc-Tag (9B11) Mouse mAb All 2279 3756 9483 2233

靶点特异抗体

No. Unconjugated Antibody Reactivity Alexa 488 Alexa 555 Alexa 594 Alexa 647
No. Unconjugated Antibody Reactivity Alexa 488 Alexa 555 Alexa 594 Alexa 647
2855 Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb H, M, R, Mk, Dm 2846     5123
4970 β-Actin (13E5) Rabbit mAb H, M, R, Mk, B, Pg, (Hm, C, Dg, Hr) 8844 8046 9470 8584
3903 AFP (3H8) Mouse mAb H 7741 7800 7877 7765
2965 Phospho-Akt (Thr308) (C31E5E) Rabbit mAb H, M, R, Hm, Mk 2918     3375
4060 Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb H, M, R, Hm, Mk, Dm, Z, B, (C, X, Dg, Pg) 4071     4075
4058 Phospho-Akt (Ser473) (193H12) Rabbit mAb H, M, R 2336     2337
4691 Akt (pan) (C67E7) Rabbit mAb H, M, R, Mk, Dm, (Pg) 5084     5186
2966 Akt (5G3) Mouse mAb H, M, R, Hm 2917     2944
5153 Androgen Receptor (D6F11) XP® Rabbit mAb H 7395 8956 8938 7397
2914 Phospho-Aurora A (Thr288)/Aurora B (Thr232)/Aurora C (Thr198) (D13A11) XP® Rabbit mAb H, M, R 8525      
2827 Phospho-Bcl-2 (Ser70) (5H2) Rabbit mAb H 2834      
2764 Bcl-xL (54H6) Rabbit mAb H, M, R, Mk 2767      
3195 E-Cadherin (24E10) Rabbit mAb H, M, (B, Dg, Pg) 3199 4295 7687 9835
9661 Cleaved Caspase-3 (Asp175) Antibody H, M, R, Mk, (B, Dg, Pg) 9669      
9579 Cleaved Caspase-3 (Asp175) (D3E9) Rabbit mAb H, (M, R, Mk, B, Pg) 9603   8172 9602
2677 β-Catenin (L54E2) Mouse mAb (IF Preferred) H, (M, R, Mk, Pg) 2849 5612   4627
3570 CD44 (156-3C11) Mouse mAb H 3516      
5664 CNPase (D83E10) XP® Rabbit mAb H, M, R 5714 5715   5716
4850 COX IV (3E11) Rabbit mAb H, R, Mk, Z, B, Pg 4853 8693 8692 7561
9198 Phospho-CREB (Ser133) (87G3) Rabbit mAb H, M, R, (Z) 9187      
4135 Cyclin B1 (V152) Mouse mAb H, M, (Hm) 4112     4118
2306 DARPP-32 (19A3) Rabbit mAb M, R, (H) 11893      
4267 EGF Receptor (D38B1) XP® Rabbit mAb H, M, Mk 5616 5108 8742 5588
2929 EpCAM (VU1D9) Mouse mAb H 5198 5488 7319 5447
5348 Phospho-c-Fos (Ser32) (D82C12) XP®Rabbit mAb H, M, R, (Hm, Mk, B, Pg, Hr) 8709     8677
2251 FosB (5G4) Rabbit mAb H, M, R 2023      
2118 GAPDH (14C10) Rabbit mAb H, M, R, Mk, (Pg) 3906 3964   3907
3670 GFAP (GA5) Mouse mAb H, M, R 3655 3656 8152 3657
3660 Glucocorticoid Receptor (D8H2) XP®Rabbit mAb H, M, R, Mk 12007      
9718 Phospho-Histone H2A.X (Ser139) (20E3) Rabbit mAb H, M, R, Mk 9719 8228   9720
3377 Phospho-Histone H3 (Ser10) (D2C8) XP® Rabbit mAb H, M, R, Mk, Z 3465 3475 8481 3458
9701 Phospho-Histone H3 (Ser10) Antibody H, M, R, Mk, C, Dm, Z, Sc, (X) 9708     9716
9649 Acetyl-Histone H3 (Lys9) (C5B11) Rabbit mAb H, M, R, Mk, Z, Ce, (Sc) 9683 5489 8823 4484
4473 Pan-Methyl-Histone H3 (Lys9) (D54) XP®Rabbit mAb H, M, R, Mk, (C, Dm, X, Z, B, Pg, Sc, Ce)       12086
9733 Tri-Methyl-Histone H3 (Lys27) (C36B11) Rabbit mAb H, M, R, Mk, (X, Z) 5499     12158
9728 Di-Methyl-Histone H3 (Lys27) (D18C8) XP® Rabbit mAb H, M, R, Mk       12244
9751 Tri-Methyl-Histone H3 (Lys4) (C42D8) Rabbit mAb H, M, R, Mk, Dm, Sc, (X, Z)       12064
4499 Histone H3 (D1H2) XP® Rabbit mAb H, M, R, Mk, (Hm, C, Dm, X, Z, B)       12230
3890 HS1 (D83A8) XP® Rabbit mAb (Human Specific) H       12162
9709 Phospho-HSP27 (Ser82) (D1H2) XP®Rabbit mAb H, M, (R, Hm, B, Dg, Hr)       11892
4876 HSP70 (D69) Antibody H, M, R, Mk 4837     4008
9837 ID3 (D16D10) Rabbit mAb H, (Dg) 11997     12038
4814 IκBα (L35A5) Mouse mAb (Amino-terminal Antigen) H, M, R, Mk, B, Pg, GP 5743     8993
3014 Insulin (C27C9) Rabbit mAb H, M, R 9016     9008
4543 Keratin 17 (D73C7) XP® Rabbit mAb H, M, R, (Mk, Dg)       8856
4545 Pan-Keratin (C11) Mouse mAb H, R, Mk 4523 3478   4528
9129 Ki-67 (D3B5) Rabbit mAb H, M, R 11882      
3308 c-Kit (Ab81) Mouse mAb H 3310      
4777 Lamin A/C (4C11) Mouse mAb H, M, R, Mk 8617      
2230 LEF1 (C12A5) Rabbit mAb H, M, R 8490      
8641 LIN28A (D1A1A) XP® Rabbit mAb H, M, (R, Mk) 12573     11845
4370 Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit mAb H, M, R, Hm, Mk, Mi, Dm, Z, B, Dg, Pg, Sc, (C, Ce) 4344     4284
9106 Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (E10) Mouse mAb H, M, R, Hm, Mk, Mi, Dm, Z, B, Pg 4374     4375
4695 p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb H, M, R, Hm, Mk, Mi, Dm, Z, B, Dg, Pg, Ce, (C) 4780     5376
3007 Phospho-MAPKAPK-2 (Thr334) (27B7) Rabbit mAb H, M, R, Mk 4338     4320
4511 Phospho-p38 MAPK (Thr180/Tyr182) (D3F9) XP® Rabbit mAb H, M, R, Mk, Mi, Pg, Sc, (Hm, C, Z, B)   9836 8632  
9216 Phospho-p38 MAPK (Thr180/Tyr182) (28B10) Mouse mAb H, M, R, Mk, Sc, (Z) 4551     4552
3077 Phospho-Met (Tyr1234/1235) (D26) XP®Rabbit mAb H, M, R     8564  
8198 Met (D1C2) XP® Rabbit mAb H 8494      
3329 MNDA (3C1) Rat mAb H 9283      
2983 mTOR (7C10) Rabbit mAb H, M, R, Mk, (Hr) 5043      
4903 Nanog (D73G4) XP® Rabbit mAb H, (Mk)     8750 5448
5482 Phospho-NDRG1 (Thr346) (D98G11) XP®Rabbit mAb H, M, R, Mk 6992 9851 8453 7497
4760 Nestin (Rat-401) Mouse mAb R 6994 8181 8484  
3033 Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb H, M, R, Hm, Mk, Pg, (Dg) 4886     4887
2837 Neurofilament-L (C28E10) Rabbit mAb H, M, R 8024 8039 8743 8590
2840 Oct-4A (C30A3) Rabbit mAb H, M 5177 4439   5263
2947 p21 Waf1/Cip1 (12D1) Rabbit mAb H, Mk, (Dg) 5487 8493 11850 8587
9286 Phospho-p53 (Ser15) (16G8) Mouse mAb H 9235 9481    
2527 p53 (7F5) Rabbit mAb H, Mk 5429 5395    
2524 p53 (1C12) Mouse mAb H, M, R, Mk 2015     2533
5625 Cleaved PARP (Asp214) (D64E10) XP®Rabbit mAb H, Mk 9148 6894   6987
8970 PAX5 (D19F8) XP® Rabbit mAb H, M, (X) 11849     11852
2586 PCNA (PC10) Mouse mAb H, M, R, Mk, B, Pg 8580      
5241 PDGF Receptor α (D13C6) XP® Rabbit mAb H 8871 8893 9015 5876
3501 PDI (C81H6) Rabbit mAb H, M, R, Mk 5051 8448 8615 8463
2258 PU.1 (9G7) Rabbit mAb H, M, (Mk, Pg) 2216     2240
4858 Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb H, M, R, Mk, Mi, Sc, (C, Pg) 4803 3985 9865 4851
8516 Phospho-Rb (Ser807/811) (D20B12) XP®Rabbit mAb H, M, R, Mk 4277 8957 8880 8974
4856 Phospho-S6 Ribosomal Protein (Ser235/236) (2F9) Rabbit mAb H, M, R, Mk 4854      
5364 Phospho-S6 Ribosomal Protein (Ser240/244) (D68F8) XP® Rabbit mAb H, M, R, Mk, (C, Pg) 5018   9468 5044
2317 S6 Ribosomal Protein (54D2) Mouse mAb H, M, R, Mk, Dm 5317 6989   5548
9255 Phospho-SAPK/JNK (Thr183/Tyr185) (G9) Mouse mAb H, M, R, Hm, Sc       9257
3579 Sox2 (D6D9) XP® Rabbit mAb H, (Mk, B, Dg, Hr) 5049 5179 7685 5067
  SQSTM1/p62 (D1D9E3)   8833      
4755 SSEA4 (MC813) Mouse mAb H   5835    
9167 Phospho-Stat1 (Tyr701) (58D6) Rabbit mAb H, M 9174 8183   8009
9145 Phospho-Stat3 (Tyr705) (D3A7) XP®Rabbit mAb H, M, R, Mk, (Hm, B, Pg, Hr) 4323     4324
9314 Phospho-Stat5 (Tyr694) (C71E5) Rabbit mAb H, M, (R, Mk, B) 3939     9365
2808 Survivin (71G4B7) Rabbit mAb H, M, R 2810 4580   2866
2710 Phospho-Syk (Tyr525/526) (C87C1) Rabbit mAb H, (M, R) 4349     12081
4179 α-Synuclein (D37A6) XP® Rabbit mAb M, R 5496     4639
8184 TNF-α (D1G2) Rabbit mAb (IF/Flow Preferred) H 12685     12771
2125 α-Tubulin (11H10) Rabbit mAb H, M, R, Mk, Dm, B, (Dg, Pg) 5063 5059   5046
2128 β-Tubulin (9F3) Rabbit mAb H, M, R, Mk, Z, B, (C) 3623 2116 7634 3624
3873 α-Tubulin (DM1A) Mouse mAb H, M, R, Mk 8058      
9411 Phospho-Tyrosine Mouse mAb (P-Tyr-100) All 9414     9415
5741 Vimentin (D21H3) XP® Rabbit mAb H, M, R, Mk 9854 9855 7675 9856
3165 Zap-70 (D1C10E) XP® Rabbit mAb H, M 9473      
  Zap-70         2707

反应性:

H=人  M=小鼠  R=大鼠  Hm=仓鼠 Mk=猴  C=鸡  Mi=貂  Dm=黑腹果蝇  X=非洲爪蟾 Z=斑马鱼  B=牛  Dg=狗 Pg=猪 Sc=酿酒酵母  GP=豚鼠  Hr=马 Ce=秀丽隐杆线虫All=所有物种

括号中的种属基于序列100%的同源性,预测可以反应。

Alexa Fluor®是Molecular Probes, Inc的商标。

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