Cell Signaling Technology

PTMScan® 开发服务模式抗体

CST研发的Motif抗体专注于翻译后修饰分析(PTMs)。我们在PTMScan发现服务中以定制的形式使用PTMScan许可证/ IAP Bead产品,我们为这些产品提供使用授权许可,允许我们的客户可以在自己的实验室进行PTMScan蛋白质组学分析。

无论您研究的目的是要了解特定的丝氨酸/苏氨酸激酶家族或激酶组分支,总体的酪氨酸磷酸化,蛋白乙酰化,或泛素化介导的信号传导,我们PTMScan发现服务和PTMScan许可证/ IAP Bead产品为深入的蛋白质组学分析翻译后修饰提供强大的工具。

产品如下:

  • KinomeView®抗体
  • Motif IAP Beads
  • PTMScan® 试剂盒

PTMScan - PhosphoScan®,AcetylScan®,UbiScan® - 是PTM蛋白质组学最有效的策略,我们18种PTMScan许可证/ IAP Bead产品(见下文)是定制、优化的试剂,用于免疫亲和纯化丝氨酸/苏氨酸和酪氨酸磷酸化、乙酰化、泛素化标记的多肽。PTMScan是一种基于Motif抗体的免疫亲和纯化和串联质谱的方法,以聚焦得到在蛋白质翻译后修饰上执行关键功能蛋白的定量结果。我们Motif抗体的PTM-特异性使PTMScan发现服务和使用授权许可/ IAP Bead产品成为信号调节通路蛋白质组学分析的标准。

KinomeView® and Serine/Threonine PhosphoScan® Products

Target Antibody Kit Consenus Site Cell or Tissue Type Peptides Identified
Target Antibody Kit Consenus Site Cell or Tissue Type Peptides Identified
Phospho-Akt Substrate 9614 5561 RXX(S*/T*) H1703 (human squamous cell carcinoma) 174 PDF XLS
Phospho-Akt Substrate 10001 5563 RXRXX(S*/T*) MKN-45 (human gastric cancer) 237 PDF XLS
Phospho-(Ser/Thr) AMPK Substrate 5759 5564 (L/M)XRXX(S*/T*), RXX(S*/T*) Human non-small cell lung cancer (H1650) cell line 157 PDF
Phospho-(Ser) ATM/ATR Substrate 9607   S*Q    
Phospho-(Ser/Thr) ATM/ATR Substrate 6966   (S*/T*)QG, (S*/T*)Q    
Phospho-(Ser) CDKs Substrate 9477   (K/R)S*PX(K/R) HeLa (human epithelial carcinoma) 305 PDF XLS
Phospho-(Ser/Thr) CK II Substrate 8738 12170 (S*/T*)(D/E)X(D/E) HeLa (human epithelial carcinoma) 497 PDF XLS
Phospho-MAPK/CDK Substrates 2325 4652 PXS*P, S*PX(K/R) HeLa (human epithelial carcinoma) 459 PDF XLS
Phospho-(Ser/Thr) PDK1 Docking Motif 9634   (F/K)XX(F/Y)(S*/T*)(F/Y) Mouse brain 424
Phospho-PKA Substrate 9624 5565 (K/R)(K/R)X(S*/T*) PC-12 (rat adrenal pheochromocytoma cell line) 205 PDF XLS
Phospho-(Ser) PKC Substrate 6967   (K/R)XS*X(K/R)    
Phospho-(Thr) PLK Binding Motif 5243 5566 ST*P HeLa (human epithelial carcinoma) 538 PDF XLS
Phospho-Thr-Pro Motif 3003   T*P, T*PP HeLa (human epithelial carcinoma) 578 PDF XLS
Phospho-Thr-Pro-Glu Motif 3004   T*PE, T*P HeLa (human epithelial carcinoma) 465 PDF XLS
Phospho-Thr-X-Arg Motif 2351   T*X(K/R) HeLa (human epithelial carcinoma) 82 PDF XLS

Select bead conjugated motif antibodies are available as part of the PTMScan® Proteomics System.

Tyrosine PhosphoScan® 产品

Target Antibody Bead Kit Consenus Site Cell or Tissue Type Peptides Identified
Phosphotyrosine (P-Tyr-100) 9411 1991 5636 Y* H3255 (non-small cell lung cancer) 930|PDF|XLS
Phosphotyrosine (P-Tyr-100) 8954 8803   Y*    

UbiScan® 产品

Target Antibody Bead Kit Consenus Site Cell or Tissue Type Peptides Identified
Ubiquitin Branch 3925 1990 5562 K-ε-GG HCT 116 (human colorectal carcinoma) 798|PDF|XLS

Cleaved Caspase Substrate PTMScan ® 产品

Description Antibody Kit Consenus Site
Cleaved Caspase Substrate 8698 12810 DE(T/S/A)D

AcetylScan® 产品

Target Antibody Bead Kit Consenus Site Cell or Tissue Type Peptides Identified
Acetylated-Lysine 9814 1989   Ac-K Jurkat (T cell acute lymphoblastic leukemia) 283

MethylScan® 产品

Target Antibody Bead Kit Consenus Site Cell or Tissue Type Peptides Identified
Methylated-Arginine 8015 - - Me-R Mouse Brain and Embryo 1109|PDF
XLS (results)
XLS (infomatics)
8711 - - ME-RGG
Di-Methyl Arginine
13522 13474   [R]    
Di-Methyl Arginine
13522 13563   [RG]    

 

PTMScan® 服务简介 

PTMScan® 开发服务 

PTMScan® 针对性服务