Cat. # | Size | Price | Inventory |
---|---|---|---|
8337T | 1 Kit (8 x 20 microliters) |
Product Includes | Quantity | Applications | Reactivity | MW(kDa) | Isotype |
---|---|---|---|---|---|
PKM2 (D78A4) XP® Rabbit mAb 4053 | 20 µl |
|
H M R Mk | 60 | Rabbit IgG |
GAPDH (D16H11) XP® Rabbit mAb 5174 | 20 µl |
|
H M R Mk | 37 | Rabbit IgG |
Pyruvate Dehydrogenase (C54G1) Rabbit mAb 3205 | 20 µl |
|
H M R Mk | 43 | Rabbit IgG |
Hexokinase I (C35C4) Rabbit mAb 2024 | 20 µl |
|
H M | 102 | Rabbit IgG |
Hexokinase II (C64G5) Rabbit mAb 2867 | 20 µl |
|
H M R Mk | 102 | Rabbit |
LDHA (C4B5) Rabbit mAb 3582 | 20 µl |
|
H Mk | 37 | Rabbit IgG |
PKM1/2 (C103A3) Rabbit mAb 3190 | 20 µl |
|
H M R Mk | 60 | Rabbit IgG |
PFKP (D4B2) Rabbit mAb 8164 | 20 µl |
|
H Mk | 80 | Rabbit IgG |
Anti-rabbit IgG, HRP-linked Antibody 7074 | 100 µl |
|
Goat |
Product Information
Monoclonal antibodies were produced by immunizing animals with a synthetic peptide corresponding to the respective sequences of human GAPDH, Hexokinase I, Hexokinase II, LDHA, PKM2, Pyruvate Dehydrogenase or PFKP protein.
Glycolysis is the metabolic process by which glucose is converted to pyruvate in a sequence of enzymatic steps. Hexokinase catalyzes the conversion of glucose to glucose-6-phosphate, the first step in glycolysis. Hexokinases I, II, and III are associated with the outer mitochondrial membrane and are critical for maintaining an elevated rate of aerobic glycolysis in cancer cells (Warburg effect) (1). Phosphofructokinase (PFK) catalyzes the phosphorylation of fructose-6-phosphate. Platelet-type phosphofructokinase (PFKP) is expressed in various cell types (2). Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate (3). Pyruvate kinase, a glycolytic enzyme, catalyses the conversion of phosphoenolpyruvate to pyruvate. In mammals, the M1 isoform (PKM1) is expressed in most adult tissues. The M2 isoform (PKM2), an alternatively-spliced variant of M1, is expressed during embryonic development (4). Lactate dehydrogenase (LDH) catalyzes the interconversion of pyruvate and NADH to lactate and NAD+ (5). LDHA expression is induced when the oxygen supply is too low for mitochondrial ATP production (6). The pyruvate dehydrogenase complex catalyzes the conversion of pyruvate and CoA into acetyl-CoA and CO2 in the presence of NAD+. The reaction of oxidative decarboxylation of pyruvate serves as a critical link between glycolysis and the citric acid cycle and lipid metabolism (7).
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