Cell Signaling Technology

Product Pathways - Metabolism

Oligomycin #9996

activator   AMPK   AMPK alpha   AMPKa   AMPKalpha   oligo   oligomycin   oxidative phosporylation   sc-203342   stimulator  

Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype

Species cross-reactivity is determined by western blot.

Applications Key:

Description

Oligomycin is supplied in lyophilized form.此寡霉素产品为冷冻干粉态。

Structure

Structure

Western Blotting

Western Blotting

Western blot analysis demonstrating optimal cell culture conditions for oligomycin treatment. 293 cells were cultured in the presence or absence of serum (O/N) and treated with or without Oligomycin (0.5µM, 30 min). Western blot analysis performed using Phospho-AMPKα (Thr172) (D79.5E) Rabbit mAb #4188.

Western Blotting

Western Blotting

Western blot analysis of extracts from 293 cells, untreated (-) or treated with Oligomycin (5 µM, 30 min; +), using Phospho-AMPKα (Thr172) (40H9) Rabbit mAb #2535 (upper) or AMPKα Antibody #2532 (lower).

Directions for Use

Oligomycin is soluble in DMSO, acetone or ethanol.

寡霉素在二甲亚砜(DMSO),丙酮和乙醇中可溶。

Background

During oxidative phosphorylation in mitochondria, the electron transport system pumps H+ from the matrix into the intermembrane compartment. This process generates a H+ gradient across the mitochondrial inner membrane. This H+ gradient is used to drive mitochondrial FoF1 ATPase to convert ADP to ATP. Oligomycin, an antibiotic from Streptomyces diastatochromogenes, blocks oxidative phosphorylation by binding to the Fo part of the ATPase to inhibit its activity (1-3).

在线粒体氧化磷酸化过程中,电子传递链将氢离子从线粒体基质泵到内外膜中层。这样就形成了一个跨线粒体内膜的质子梯度。质子梯度可以驱动线粒体内膜上的F0-F1 ATP合酶,将ADP转化成ATP。寡霉素,是一种淀粉酶产色链丝菌产生的抗生素,它可以通过结合到ATP合酶上的F0组分来抑制其活性,并阻断氧化磷酸化过程(1-3)。

  1. Kagawa, Y. and Racker, E. (1966) J Biol Chem 241, 2461-6.
  2. Kagawa, Y. and Racker, E. (1966) J Biol Chem 241, 2467-74.
  3. Fessenden, J.M. and Racker, E. (1966) J Biol Chem 241, 2483-9.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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