Cell Signaling Technology

Product Pathways - Apoptosis

Staurosporine #9953

alkaloid   apoptosis   apoptosis induction   CAMKII   cleaved caspase   cleaved parp   Cleaved-Caspase3   cleaved-PARP   full length PARP   leaved caspase3   mlc   MLCK   myosin light chain   neurotrophin agonist   nhibitor   PKA   PKC   PKC inhibitor   PKG   staurosporene   Streptomyces staurosporesa  

Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype

Species cross-reactivity is determined by western blot.

Applications Key:

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, untreated or Staurosporine-treated (3 hours), showing PARP cleavage as evidence of induction of apoptosis, using Cleaved PARP Antibody #9541 (upper) or PARP Antibody #9542 (lower).



Chemical structure of Staurosporine.

Directions for Use

Dissolve the lyophilized alkaloid in DMSO or Methanol. For a 1 mM solution, dissolve in 0.536 mL.



Staurosporine is an alkaloid isolated from the culture broth of Streptomyces staurosporesa. It is a potent, cell permeable protein kinase C inhibitor with an IC50 of 0.7 nM. At higher concentration (1-20 nM), staurosporine also inhibits other kinases such as PKA, PKG, CAMKII and Myosin light chain kinase (MLCK) (1). At 50-100 nM, it is a functional neurotrophin agonist, promoting neurite outgrowth in neuroblastoma, pheochromocytoma and brain primary neuronal cultures. At 0.2- 1 μM, staurosporine induces cell apoptosis (2,3).

Staurosporine(星孢菌素)是链霉菌肉汤培养液中提取的生物碱。它是强有效的细胞渗透的蛋白酶C抑制剂,IC50是0.7nM。高浓度(1-20nM)时,staurosporine 也可以抑制其它酶的活性,例如PKA、PKG、CAMKII和球蛋白轻链酶(1)。浓度为50-100nM时,它是作为神经营养因子的激动剂,促进神经母细胞瘤、嗜铬细胞瘤和脑原发神经元神经轴突外生。浓度为0.2-1uM时,staurosporine 诱导细胞凋亡。(2,3)

  1. Ruegg, U.T. and Burgess, G.M. (1989) Trends Pharmacol. Sci. 10, 218-220.
  2. Couldwell , W. T. et al. (1994) FEBS Lett. 345, 43-46.
  3. Yue, T. L. et al. (1998) J. Mol. Cell. Cardiol. 30, 495-507.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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