Cell Signaling Technology

Product Pathways - Metabolism

AICAR #9944

5-aminoimidazole-4-carboxamide-1-b-D-ribofuranoside   Acadesine   activator   adenosine   AICA-Riboside   AICAR   AMP   amp-activated-protein-kinase   AMPK   AMPK alpha   AMPKa   AMPKalpha   stimulator   Z-Riboside  

No. Size Price
9944S 44 mg ( 12 assays ) ¥1,413.00 现货查询 购买询价
9944 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype

Species cross-reactivity is determined by western blot.

Applications Key:


AICAR is supplied at 250 mM concentration. For a 0.5 mM working concentration, dilute 1:500. For a 2 mM working concentration, dilute 1:125. Treat cells with desired concentration for 5-60 minutes. Please note: Appearance of AICAR solution should be a clear to yellow/brown solution without precipitation. If precipitation is observed, warm tightly capped vial at 37C until AICAR goes into solution. Agitation of warmed solution by vortex may be necessary.

AICAR产品浓度为250 mM。对于0.5 mM的工作浓度,将原液按1:500稀释,对于2 mM的工作浓度,按1:125稀释。细胞应于预期浓度下处理5-60min。需要注意的是:AICAR是清澈透明的呈黄棕色的溶液,不应有沉淀。如果发现有沉淀,将盖子拧紧后,用体温加热小瓶,直到沉淀溶解。必要时,可以边加热边摇动溶液,促进其溶解。

Western Blotting

Western Blotting

Western Blot analysis of extracts from C2C12 cells, cultured in the presence or absence of serum (O/N) and treated with or without AICAR (0.5 mM, 30 min), using Phospho-AMPKalpha (Thr172) (40H9) Rabbit mAb #2535.

Directions for Use

This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures. AICAR is supplied at 250 mM in water. Thaw completely before use. For a 0.5 mM working concentration, dilute 1:500. For a 2 mM working concentration, dilute 1:125. Treat cells with desired concentration for 5-60 minutes.

此产品仅用于体外实验,不适用于动物和人体研究。此产品同样不适用于治疗和诊断。AICAR以250 mM浓度的水溶液提供。使用前应充分解冻融。对于0.5 mM的工作浓度,将原液按1:500稀释,对于2 mM的工作浓度,按1:125稀释。细胞于预期浓度下处理5-60min。


AICAR (5-Aminoimidazole-4-carboxyamide ribonucleoside) is an adenosine analog taken up by muscle and phosphorylated to form 5-aminoimidazole-4-carboxamide-1--D-ribofuranosyl-5''-monophosphate (ZMP), which stimulates AMPK activity and glucose transport in skeletal muscle (1). AICAR has been used in studies measuring glucose uptake, diabetes and insulin resistance, and energy regulation during exercise. AICAR acts by entering nucleoside pools and significantly increasing levels of adenosine during periods of ATP breakdown (2).


  1. Sakoda, H. et al. (2002) Am. J. Physiol. Endocrinol. Metab. 282, E1239-E1244.
  2. Mullane, K. et al. (1993) Trends Cardiovasc. Med. 3, 227-234.
  3. Jakobsen, S. N. et al. (2001) J. Biol. Chem. 276, 46912-46916.
  4. Jessen, N. et al. (2003) J. Appl. Physiol. 94, 1373-1379.
  5. Giri, S. et al. (2004) J. Neurosci. 24, 479-487.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

用户评论 --- 共 0


我要参与评论 :