Cell Signaling Technology

Product Pathways - Chromatin Regulation / Epigenetics

Histone Deacetylase (HDAC) Antibody Sampler Kit #9928

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REACTIVITY

No. Size Price
9928T 1 Kit ( 6 x 20 µl ) ¥5,301.00 现货查询 购买询价
Kit Includes Quantity Applications Reactivity Homology† MW (kDa) Isotype
Phospho-HDAC4 (Ser246)/HDAC5 (Ser259)/HDAC7 (Ser155) (D27B5) Rabbit mAb #3443 20 µl W, H,M, 140, 124 Rabbit IgG
HDAC3 (7G6C5) Mouse mAb #3949 20 µl W,IP,IF-IC, H,M,R,Mk, 49 Mouse IgG2a
HDAC2 (3F3) Mouse mAb #5113 20 µl W,IP,IF-IC, H,M,R,Mk, 60 Mouse IgG1
HDAC1 (10E2) Mouse mAb #5356 20 µl W,IP, H,M,R,Mk, 62 Mouse IgG1
Anti-rabbit IgG, HRP-linked Antibody #7074 100 µl W, Goat
Anti-mouse IgG, HRP-linked Antibody #7076 100 µl W, Horse
HDAC6 (D2E5) Rabbit mAb #7558 20 µl W,IP,IHC-P,IF-IC, H,Mk, 160 Rabbit IgG
HDAC4 (D15C3) Rabbit mAb #7628 20 µl W,IP, H,M,R,Mk, 140 Rabbit IgG

Specificity / Sensitivity

The Histone Deacetylase (HDAC) Antibody Sampler Kit provides a fast and economical means to evaluate the endogenous levels of HDACs. The kit contains enough primary and secondary antibodies to perform four mini-blot experiments. 
 Each antibody in the Histone Deacetylase (HDAC) Antibody Sampler Kit detects endogenous levels of its target and does not recognize other family members.

Histone Deacetylase (HDAC) Antibody Sampler Kit提供了一个快速经济的方法去检测内源性HDACs蛋白水平。该试剂盒包含的一抗和二抗可以保证四次免疫印迹实验。在Histone Deacetylase (HDAC) Antibody Sampler Kit中每个抗体可检测内源性的靶蛋白,并且不能与其它家族成员识别。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to the carboxy terminus of human HDAC5 protein. Antibodies are purified by protein A and peptide affinity chromatography. Monoclonal antibodies are produced by immunizing animals with a recombinant protein specific to the carboxy terminus of human HDAC6, the amino terminus of human HDAC4 protein or with synthetic peptides to the carboxy-terminal residues of human HDAC1 and HDAC2 proteins. HDAC3 (7G6C5) monoclonal antibody is produced by immunizing animals with recombinant human HDAC3 protein.

通过人工合成人源HDAC5蛋白羧基端相应的多肽片段去免疫动物从而制备出多克隆抗体。通过蛋白A和多肽亲和层析纯化抗体。通过重组特异性的人源HDAC6羧基端、人源HDAC4蛋白氨基端或人源HDAC1和HDAC2蛋白羧基端相应的多肽片段去免疫动物从而制备出单克隆抗体。HDAC3 (7G6C5) monoclonal antibody通过重组人源HDAC3蛋白去免疫动物从而制备。

Description

The Histone Deacetylase (HDAC) Antibody Sampler Kit provides a fast and economical means to evaluate the endogenous levels of HDACs. The kit contains enough primary and secondary antibodies to perform four Western blot experiments.

Histone Deacetylase (HDAC) Antibody Sampler Kit提供一个快速经济的方法去检测内源性HDACs蛋白。该试剂盒包含的一抗和二抗可以保证四次免疫印迹实验。

Western Blotting

Western Blotting

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO* is added and emits light during enzyme catalyzed decomposition.

Western Blotting

Western Blotting

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO* is added and emits light during enzyme catalyzed decomposition.

Western Blotting

Western Blotting

Western blot analysis of lysates from various cell lines using Histone Deacetylase 5 (HDAC5) Antibody #2082.

使用Histone Deacetylase 5 (HDAC5) Antibody #2082,免疫印迹(Western blot)分析不同细胞中Histone Deacetylase 5 (HDAC5)的蛋白水平。

Western Blotting

Western Blotting

Western blot analysis of extracts from DO11.10 thymocyte hybridoma cells, either untreated or treated for 1 h with TPA (0.2 uM) and ionomycin (0.33 uM), using Phospho-HDAC4 (Ser246)/HDAC5 (Ser259)/HDAC7 (Ser155) (D27B5) Rabbit mAb. Phospho-specificity of the antibody was determined by treating cell extracts with lambda phosphatase. Total HDAC proteins were detected using Histone Deacetylase 4 (HDAC4) Antibody #2072, Histone Deacetylase 5 (HDAC5) Antibody #2082 and Histone Deacetylase 7 (HDAC7) Antibody #2882.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using HDAC3 (7G6C5) Mouse mAb.

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells using HDAC3 (7G6C5) Mouse mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red).

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells using HDAC1 (10E2) Mouse mAb (green). Actin filaments were labeled with DY-554 phalloidin (red).

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa, NIH/3T3, and H-4-II-E cells using HDAC1 (10E2) Mouse mAb.

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells using HDAC2 (3F3) Mouse mAb (green). Actin filaments were labeled using DY-554 Phalloidin (red).

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using HDAC2 (3F3) Mouse mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa, NIH/3T3, and H-4-II-E cells using HDAC1 (10E2) Mouse mAb #5356.

使用HDAC1 (10E2) Mouse mAb #5356,免疫印迹(Western blot)分析HeLa、NIH/3T3和H-4-II-E细胞中HDAC1 (10E2)的蛋白水平。

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using HDAC2 (3F3) Mouse mAb #5113.

使用HDAC2 (3F3) Mouse mAb #5113,免疫印迹(Western blot)分析不同细胞中HDAC2 (3F3)的蛋白水平。

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using HDAC3 (7G6C5) Mouse mAb #3949.

使用HDAC3 (7G6C5) Mouse mAb #3949,免疫印迹(Western blot)分析不同细胞中HDAC3 (7G6C5)的蛋白水平。

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using HDAC4 (D15C3) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using HDAC6 (D2E5) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using HDAC6 (D2E5) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using HDAC6 (D2E5) Rabbit mAb.

IF-IC

IF-IC

Confocal immunofluorescent analysis of A549 cells, untreated (left) or treated with MG132 (5 μM, 24 hr; right), using HDAC6 (D2E5) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5®#4084 (fluorescent DNA dye).

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using HDAC4 (D15C3) Rabbit mAb #7628.

使用HDAC4 (D15C3) Rabbit mAb #7628,免疫印迹(Western blot)分析不同细胞中HDAC4 (D15C3)的蛋白水平。

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using HDAC6 (D2E5) Rabbit mAb #7558.

使用HDAC6 (D2E5) Rabbit mAb #7558,免疫印迹(Western blot)分析不同细胞中HDAC6 (D2E5)的蛋白水平。

Western Blotting

Western Blotting

Western blot analysis of extracts from DO11.10 thymocyte hybridoma cells, either untreated or treated for 1 h with TPA (0.2 uM) and ionomycin (0.33 uM), using Phospho-HDAC4 (Ser246)/HDAC5 (Ser259)/HDAC7 (Ser155) (D27B5) Rabbit mAb #3443. Phospho-specificity of the antibody was determined by treating cell extracts with lambda phosphatase. Total HDAC proteins were detected using Histone Deacetylase 4 (HDAC4) Antibody #2072, Histone Deacetylase 5 (HDAC5) Antibody #2082 and Histone Deacetylase 7 (HDAC7) Antibody #2882.

Western Blotting

Western Blotting

Western blot analysis of NIH/3T3 cells using Phospho-HDAC4 (Ser246)/HDAC5 (Ser259)/HDAC7 (Ser155) (D27B5)

Rabbit mAb #3443, HDAC4 (D8T3Q) Rabbit mAb #15164, HDAC5 (D1J7V) Rabbit mAb #20458, and HDAC7 (D4E1L) Rabbit mAb #33418. To show the phospho-specificity of the antibody, the blot was mock treated (-) or treated (+) with calf intestinal phosphatase (CIP) and lambda phosphatase. As expected, signal from the Phospho-HDAC4 (Ser246)/HDAC5 (Ser259)/HDAC7 (Ser155) (D27B5) Rabbit mAb is lost after treatment of the blot with phosphatase.

Background

Acetylation of the histone tail causes chromatin to adopt an "open" conformation, allowing increased accessibility of transcription factors to DNA. The identification of histone acetyltransferases (HATs) and their large multiprotein complexes has yielded important insights into how these enzymes regulate transcription (1,2). HAT complexes interact with sequence-specific activator proteins to target specific genes. In addition to histones, HATs can acetylate nonhistone proteins, suggesting multiple roles for these enzymes (3). In contrast, histone deacetylation promotes a "closed" chromatin conformation and typically leads to repression of gene activity (4). Mammalian histone deacetylases can be divided into three classes on the basis of their similarity to various yeast deacetylases (5). Class I proteins (HDACs 1, 2, 3, and 8) are related to the yeast Rpd3-like proteins, those in class II (HDACs 4, 5, 6, 7, 9, and 10) are related to yeast Hda1-like proteins, and class III proteins are related to the yeast protein Sir2. Inhibitors of HDAC activity are now being explored as potential therapeutic cancer agents (6,7).

组蛋白尾部的乙酰化可引起染色质成为松散的构象,这允许转录因子更易接近DNA。组蛋白去乙酰化转移酶(histone acetyltransferases,HATs)的鉴定和它们的多种蛋白复合物在它们怎样酶促调节转录中已经有了重要的见解(1,2)。HAT复合物可与特异性靶基因上序列特异的激活蛋白发生相互作用。除了组蛋白之外,HATs能够使非组蛋白乙酰化,这就认为这些酶的多种功能(3)。与此相反,组蛋白去乙酰化可促进一个致密的染色质构象,并且典型地导致基因活性的抑制(4)。哺乳动物组蛋白去乙酰化酶能按照它们的类似与多种酵母去乙酰化酶划分成三类(5)。Class I蛋白 (HDACs 1、2、3和8)是与酵母Hda1样蛋白相关,并且class III蛋白是与酵母Sir2相关。目前HDAC活性的抑制剂已经被认为潜在的治疗癌症的药物(6,7)。

  1. Marmorstein, R. (2001) Cell Mol Life Sci 58, 693-703.
  2. Gregory, P.D. et al. (2001) Exp Cell Res 265, 195-202.
  3. Liu, Y. et al. (2000) Mol Cell Biol 20, 5540-53.
  4. Cress, W.D. and Seto, E. (2000) J Cell Physiol 184, 1-16.
  5. Gray, S.G. and Ekström, T.J. (2001) Exp Cell Res 262, 75-83.
  6. Thiagalingam, S. et al. (2003) Ann. N.Y. Acad. Sci. 983, 84-100.
  7. Vigushin, D.M. and Coombes, R.C. (2004) Curr Cancer Drug Targets 4, 205-18.

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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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