Cell Signaling Technology

Product Pathways - Companion Products

Calyculin A #9902

akt   Cal A   cala   general   motif   Okadaic acid   phosphatase   Phosphatase Inhibitor   phospho   PP1   PP2   PP2A  

Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype

Species cross-reactivity is determined by western blot.

Applications Key:

Description

Calyculin A (Serine/Threonine Phosphatase Inhibitor) is a more potent phosphatase inhibitor than Okadaic acid (2). As shown by Western blot, treatment of cells with 100 nM Calyculin A for 30 minutes induces threonine phosphorylation, detected by Phospho-Threonine-Polyclonal Antibody #9381. IC50 values for inhibitory activity against PP1 are approximately 2 nM. IC50 values for inhibitory activity against PP2A are approximately 0.5 -1.0 nM. 花萼海绵诱癌素A丝氨酸/苏氨酸抑制剂)与冈田软海绵酸相比较,是一种更加有潜力的磷酸酶抑制剂(2)。Western blot实验结果显示,用100nM花萼海绵诱癌素A处理细胞30分钟后,采用苏氨酸磷酸化多克隆抗体#9381可以检测到苏氨酸发生磷酸化。对抗PP1的抑制活性IC50值大约为2nM。对抗PP2A的抑制活性IC50值大约为0.5-1.0nM。

Western Blotting

Western Blotting

Western blot analysis of extracts from A431 cells treated with varied concentrations of Okadaic Acid or calyculin A (30 minutes) using P-Thr-Polyclonal #9381. Wstern blot方法检测不同浓度冈田酸和花萼海绵体诱癌素A处理30分钟后的A431细胞提取物,使用的抗体为P-Thr-Polyclonal #9381。

Western Blotting

Western Blotting

Western blot analysis of extracts from Jurkat cells, untreated or calyculin A-treated (0.1 µM for 45 minutes prior to lysis), using P-Thr-Polyclonal #9381. Proteins were separated by 2D electrophoresis prior to blotting. Western blot方法检测未处理和裂解前用0.1 µM 花萼海绵体诱癌素A处理45分钟的Jurkat细胞提取物,使用的抗体为P-Thr-Polyclonal #9381。蛋白样品先由2D电泳分离后再进行western blot分析。

Directions for Use

Calyculin A is supplied as a lyophilized clear film. For 10 µM stock, reconstitute in 1 ml DMSO. Store in aliquots at -20ºC in the dark. Treat cells with 50-100 nM calyculin A for 5-30 minutes. Store in aliquots tightly sealed (unopened) at -20ºC in the dark. See MSDS. 
 
 This compound is sold only for use in extremely dilute solutions for biological research. No other use is intended and any other use involves substantial hazards. This compound should never be handled in powder or aerosol form or in any other form susceptible to uncontrolled release in the laboratory, even in very small quantities. 本产品为冻干粉,10µM储存可溶于1毫升DMSO。少量分装后-20度避光保存。用50-100nM花萼海绵诱癌素A处理细胞5-30分钟。分装后-20度避光密封保存。请查看MSDS。 本化合物仅用于生物研究,禁止用于其它或者涉及重大危害的用途。本化合物严禁以粉末或者喷雾形式或者其它在实验室易于释放的形式处理,即使是很小的剂量。

Background

Calyculin A inhibits the activity of protein phosphatases PP1 and PP2A (1,2). Unlike Okadaic acid, which reduces PP2A activity but has little effect on PP1 activity, Calyculin A inhibits both phosphatases (1). Neither Calyculin A nor Okadaic acid inhibit acid or alkaline phosphatases or phospho- tyrosine protein phosphatases (2).花萼海绵诱癌素A可以抑制PP1和PP2A蛋白磷酸酶的活性(1,2)。冈田软海绵酸仅降低PP2A的活性而对PP1的活性几乎没有影响,与其不同的是花萼海绵诱癌素A可以抑制这两种磷酸酶(1)。花萼海绵诱癌素A和冈田软海绵酸都不能抑制酸性或碱性磷酸酶或者酪氨酸蛋白磷酸酶的活性(2)。

  1. Resjö, S. et al. (1999) Biochem J 341 ( Pt 3), 839-45.
  2. Ishihara, H. et al. (1989) Biochem Biophys Res Commun 159, 871-7.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

用户评论 --- 共 0

该产品暂无评论!

我要参与评论 :

如要参与评论请先登录网站

还没有网站账户?去注册一下吧

Products

 

Applications