Cell Signaling Technology

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Phospho-Numb (Ser276) (D5C2) Rabbit mAb #9878

No. Size Price
9878S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
9878 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 78 Rabbit IgG
IF-IC 1:200

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IF-IC=Immunofluorescence (Immunocytochemistry),


Species predicted to react based on 100% sequence homology: Mouse, Rat, Chicken, Xenopus, Zebrafish, Bovine, Horse,

Specificity / Sensitivity

Phospho-Numb (Ser276) (D5C2) Rabbit mAb recognizes endogenous levels of numb protein only when phosphorylated at Ser276.

Phospho-Numb (Ser276) (D5C2) Rabbit mAb兔单抗只识别内源性的Numb蛋白。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser276 of human numb protein.


Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa and U-87 MG cells, untreated or treated with either λ phosphatase or TPA #4174 (200 nM, 30 min), using Phospho-Numb (Ser276) (D5C2) Rabbit mAb (upper) or Numb (C29G11) Rabbit mAb #2756 (lower).



Confocal immunofluorescent analysis of HeLa cells, untreated (left) or treated with either TPA #4174 (center) or TPA and λ phosphatase (right), using Phospho-Numb (Ser276) (D5C2) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).


Numb contains an amino-terminal phosphotyrosine-binding (PTB) domain and carboxy-terminal endocytic binding motifs for α-adaptin and EH (Eps15 homology) domain-containing proteins, indicating a role in endocytosis (1,2). There are four mammalian Numb splicing isoforms that are differentially expressed and may have distinct functions (3-5). Numb acts as a negative regulator of Notch signaling by promoting ubiquitination and degradation of Notch (6). The protein is asymmetrically segregated into one daughter cell during cell division, producing two daughter cells with different responses to Notch signaling and different cell fates (7,8). The localization of Numb can also be regulated by G-protein coupled receptor (GPCR) and PKC signaling (9).

Numb包含一个氨基末端磷酸化酪氨酸结合(PTB)结构域和C末端对α-衔接蛋白和EH(Eps15 同源物)结构域包含蛋白的内吞结合基序,显示在细胞吞噬中的作用(1,2)有四个哺乳动物Numb剪接异构体,差异表达,并有各自独特功能(3-5)。Numb通过促进Notch泛素降解对Notch信号通路起负向调控作用(6),蛋白在细胞分裂时不对称的入子细胞,产生的两个子细胞对Notch信号和细胞命运都有不同反应(7,8)。Numb可能被G蛋白偶联受体和PKC信号调控(9)。

Numb can be phosphorylated at several sites including Ser7, Ser276, and Ser295. Phosphorylation at these sites regulates asymmetric membrane localization of Numb and integrin endocytosis (10-12).

Numb的不同位点可以被磷酸化,包括Ser7, Ser276和Ser295。在这些位点的磷酸化可调控Numb的不对称膜定位和整合内吞作用。

  1. Berdnik, D. et al. (2002) Dev. Cell 3, 221-231.
  2. Santolini, E. et al. (2000) J. Cell Biol. 151, 1345-1352.
  3. Dho, S.E. et al. (1999) J. Biol. Chem. 274, 33097-33104.
  4. Verdi, J.M. et al. (1999) Proc. Natl. Acad. Sci. USA 96, 10472-10476.
  5. Toriya, M. et al. (2006) Dev. Neurosci. 28, 142-155.
  6. McGill, M.A. and McGlade, C.J. (2003) J. Biol. Chem. 278, 23196-23203.
  7. Verdi, J.M. et al. (1996) Curr. Biol. 6, 1134-1145.
  8. Reugels, A.M. et al. (2006) Dev. Dyn. 235, 934-948.
  9. Dho, S.E. et al. (2006) Mol. Biol. Cell 17, 4142-4155.
  10. Nishimura, T. and Kaibuchi, K. (2007) Dev Cell 13, 15-28.
  11. Smith, C.A. et al. (2007) EMBO J 26, 468-80.
  12. Wirtz-Peitz, F. et al. (2008) Cell 135, 161-73.

Application References

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