Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

Vimentin (D21H3) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate) #9854

Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
F 1:50 Human,Mouse,Rat,Monkey, Endogenous Rabbit IgG
IF-IC 1:800

Species cross-reactivity is determined by western blot.

Applications Key: F=Flow Cytometry, IF-IC=Immunofluorescence (Immunocytochemistry),

Specificity / Sensitivity

Vimentin (D21H3) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate) detects endogenous levels of total vimentin protein.

Vimentin (D21H3) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate)兔单抗检测内源性vimentin总蛋白。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg45 of human vimentin protein.

通过人工合成人源vimentin蛋白Arg45位点周围相应的多肽片段去免疫动物从而制备出单克隆抗体。

Description

This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 488 fluorescent dye and tested in-house for direct flow cytometry and immunofluorescent analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Vimentin (D21H3) XP® Rabbit mAb #5741.

Cell Signaling Technology antibody连接着Alexa Fluor® 488 fluorescent dye,并且直接用流式细胞仪和免疫荧光分析方法内部检测人类细胞。该抗体被期望与非连接的Vimentin (D21H3) XP® Rabbit mAb #5741有相同的物种交叉反应。

IF-IC

IF-IC

Confocal immunofluorescent analysis of SNB19 cells using Vimentin (D21H3) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate) (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

激光共聚焦免疫荧光分析SNB19 细胞,使用的抗体是Vimentin (D21H3) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate) #9854 (绿色)。肌动蛋白纤维用DY-554 phalloidin 标记(红色)。蓝色伪彩= DRAQ5® #4084(荧光DNA染料)。

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of HeLa cells, using Vimentin (D21H3) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate) (green) compared to Rabbit (DA1E) mAb IgG XP® Isotype Control (Alexa Fluor® 488 Conjugate) #2975 (red).

流式细胞术分析Hela细胞,使用的抗体是 Vimentin (D21H3) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate) (绿色),与之比较的是Rabbit (DA1E) mAb IgG XP® Isotype Control (Alexa Fluor® 488 Conjugate) #2975(红色)。

Background

The cytoskeleton consists of three types of cytosolic fibers: microfilaments (actin filaments), intermediate filaments, and microtubules. Major types of intermediate filaments are distinguished by their cell specific expression: cytokeratins (epithelial cells), glial fibrillary acidic protein (GFAP) (glial cells), desmin (skeletal, visceral, and certain vascular smooth muscle cells), vimentin (mesenchyme origin), and neurofilaments (neurons). GFAP and vimentin form intermediate filaments in astroglial cells and modulate their motility and shape (1). In particular, vimentin filaments are present at early developmental stages, while GFAP filaments are characteristic of differentiated and mature brain astrocytes. Thus, GFAP is commonly used as a marker for intracranial and intraspinal tumors arising from astrocytes (2). Vimentin is present in sarcomas, but not carcinomas, and its expression is examined in conjunction with that of other markers to distinguish between the two (3). Vimentin's dynamic structural changes and spatial re-organization in response to extracellular stimuli help to coordinate various signaling pathways (4). Phosphorylation of vimentin at Ser56 in smooth muscle cells regulates the structural arrangement of vimentin filaments in response to serotonin (5,6). Remodeling of vimentin and other intermediate filaments is important during lymphocyte adhesion and migration through the endothelium (7).

细胞骨架由三种细胞基质纤维组成:微丝(actin filaments)、中间纤维和微管。中间纤维的主要类型是有区别的,且它在特别的细胞中表达:细胞角蛋白(epithelial细胞)、胶质原纤维酸性蛋白 或GFAP(glial细胞)、desmin (skeletal、viscera和某种vascular smooth muscle 细胞)、波形蛋白(间充质来源)和神经纤维细丝(neurons)。GFAP和vimentin蛋白在星形胶质细胞瘤细胞中形成中间纤维,并且调节它们的活性和形状(1)。尤其,vimentin filaments在早期发育阶段出现,而GFAP filaments是分化的和成熟的大脑星形胶质细胞的特征。因此,GFAP普遍被用作来自星形胶质细胞颅内和椎管内肿瘤上升的标记物 (2)。Vimentin出现在恶性毒瘤中,但不出现癌中,因此相对于其它标记物该蛋白的表达被检测用于区别两种形式的肿瘤(3)。在外界刺激下,Vimentin的动力结构的改变和空间的重组有助于协调不同的信号通路(4)。在平滑肌细胞中血清素刺激下,vimentin蛋白在Ser56位点的磷酸化调节vimentin微丝的结构性布置(5,6)。在淋巴细胞的黏附和迁移期间,vimentin的重塑和其它中间纤维对穿过内皮是起着重要作用(7)。

  1. Eng, L.F. et al. (2000) Neurochem Res 25, 1439-51.
  2. Goebel, H.H. et al. (1987) Acta Histochem Suppl 34, 81-93.
  3. Leader, M. et al. (1987) Histopathology 11, 63-72.
  4. Helfand, B.T. et al. (2004) J Cell Sci 117, 133-41.
  5. Tang, D.D. et al. (2005) Biochem J 388, 773-83.
  6. Fomina, I.G. et al. (1990) Klin Med (Mosk) 68, 125-7.
  7. Nieminen, M. et al. (2006) Nat Cell Biol 8, 156-62.
  8. Yamaguchi, T. et al. (2005) J Cell Biol 171, 431-6.
  9. Oguri, T. et al. (2006) Genes Cells 11, 531-40.
  10. Zhu, Q.S. et al. (2011) Oncogene 30, 457-70.
  11. Xue, G. and Hemmings, B.A. (2013) J Natl Cancer Inst 105, 393-404.

Application References

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Protocols

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

The Alexa Fluor dye antibody conjugates in this product are sold under license from Life Technologies Corporation for research use only, except for use in combination with DNA microarrays. The Alexa Fluor® dyes (except for Alexa Fluor® 430 dye) are covered by pending and issued patents. Alexa Fluor® is a registered trademark of Molecular Probes, Inc.

DRAQ5 is a registered trademark of Biostatus Limited.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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