Cell Signaling Technology

Product Pathways - Adhesion

Integrin α5 (D7B7G) Rabbit mAb #98204

No. Size Price
98204S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
98204 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Hamster,Monkey, Endogenous 150 Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

Integrin α5 (D7B7G) Rabbit mAb recognizes endogenous levels of total Integrin α5 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly117 of human Integrin α5 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Integrin α5 (D7B7G) Rabbit mAb (upper) or α-Actinin (D6F6) XP® Rabbit mab #6487 (lower).

Background

Integrins are α/β heterodimeric cell surface receptors that play a pivotal role in cell adhesion and migration, as well as in growth and survival (1,2). The integrin family contains at least 18 α and 8 β subunits that form 24 known integrins with distinct tissue distribution and overlapping ligand specificities (3). Integrins not only transmit signals to cells in response to the extracellular environment (outside-in signaling), but also sense intracellular cues to alter their interaction with the extracellular environment (inside-out signaling) (1,2).

Integrin α5/β1 is involved in multiple biological processes including embryonic development, angiogenesis and tumor metastasis (4,5). By interaction with its fibronectin ligand, α5/β1 transduces signals that regulate cell adhesion, migration, matrix assembly and cytoskeletal organization (6).

  1. Liu, S. et al. (2000) J Cell Sci 113 ( Pt 20), 3563-71.
  2. Hood, J.D. and Cheresh, D.A. (2002) Nat Rev Cancer 2, 91-100.
  3. van der Flier, A. and Sonnenberg, A. (2001) Cell Tissue Res 305, 285-98.
  4. Kabir-Salmani, M. et al. (2004) Mol Hum Reprod 10, 91-7.
  5. Kim, S. et al. (2000) J Biol Chem 275, 33920-8.
  6. Miyamoto, S. et al. (1995) J Cell Biol 131, 791-805.

Application References

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Protocols

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

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Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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