Cell Signaling Technology

Product Pathways - Motif Antibodies

Acetylated-Lysine (Ac-K2-100) MultiMab™ Rabbit mAb mix #9814

acetyl lysine   acetylated lysine   acetylation   motif  

No. Size Price
9814S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
9814 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Monkey,All Species Expected, Endogenous Rabbit IgG
IP 1:100
ChIP 1:50
E-P 1:1000

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, ChIP=Chromatin IP, E-P=Peptide ELISA (DELFIA),

Specificity / Sensitivity

Acetylated-Lysine (Ac-K2-100) Rabbit mAb detects proteins post-translationally modified by acetylation on the ε-amine groups of lysine residues. The antibody recognizes acetylated lysine in a wide range of sequence contexts. It has been demonstrated to recognize acetylated histones, p53, CBP, PCAF and chemically acetylated BSA. The antibody has been shown to react with as little as 0.04 ng of chemically acetylated BSA while not recognizing up to 25 µg of non-acetylated BSA. (U.S. Patent No's.: 6,441,140; 6,982,318; 7,259,022; 7,344,714; U.S.S.N. 11,484,485; and all foreign equivalents.)

Acetylated-Lysine (Ac-K2-100) Rabbit mAb乙酰化赖氨酸兔单抗识别转录后修饰为赖氨酸残基ε-氨基乙酰化的蛋白质。此抗体可识别不同背景序列中的乙酰化赖氨酸。研究显示它可以识别乙酰化的组蛋白、p53、CBP、PCAF和化学乙酰化的BSA。研究显示抗体可以与少到0.04ng的化学乙酰化的BSA反应,却不能与非乙酰化的多到25ug的BSA反应。(美国专利号:6,441,140、6,982,318、7,259,022、7,344,714;U.S.S.N. 11,484,485;及所有国外相应专利)

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic acetyl-lysine peptide library. The antibody is formulated from two rabbit monoclones in order to cover broad range of reactivity.

该单克隆抗体用合成的乙酰化赖氨酸底物库肽段免疫动物制备。该抗体是两个兔单克隆的混合,以便可以覆盖更大的反应范围。

Chromatin IP

Chromatin IP

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 HeLa cells and either 10 μl of Acetylated-Lysine (Ac-K2-100) Rabbit mAb #9814 or 2 μl of Normal Rabbit IgG #2729, using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human GAPDH Exon 1 Primers #5516, SimpleChIP® Human RPL30 Exon 3 Primers #7014, SimpleChIP® Human MYT-1 Exon 1 Primers #4493, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

使用SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003试剂盒,将4 x 10^6 HeLa细胞的染色质,与10 μl Acetylated-Lysine (Ac-K2-100) Rabbit mAb #9814或者2 μl of Normal Rabbit IgG #2729进行染色质免疫沉淀。使用人SimpleChIP® Human GAPDH Exon 1 Primers #5516, SimpleChIP® Human RPL30 Exon 3 Primers #7014, SimpleChIP® Human MYT-1 Exon 1 Primers #4493和SimpleChIP® Human α Satellite Repeat Primers #4486进行real-time PCR用来定量富集的DNA。每个样本中免疫沉淀的DNA用相对于输入对照的染色质的量来描述,输入对照的量相当于1。

Western Blotting

Western Blotting

Western blot analysis of extracts from COS and HeLa cells, untreated or TSA-treated (1 µM, 6 hours), using Acetylated-Lysine (Ac-K2-100) Rabbit mAb.

对COS和Hela细胞,未处理或1uM TSA处理6小时,使用Acetylated-Lysine (Ac-K2-100) Rabbit mAb进行Western blot分析。

Western Blotting

Western Blotting

Western blot analysis of extracts from various mouse tissues using Acetylated-Lysine (Ac-K2-100) Rabbit mAb.

对多个小鼠组织使用Acetylated-Lysine (Ac-K2-100) Rabbit mAb进行Western blot分析。

Background

Acetylation of lysine, like phosphorylation of serine, threonine or tyrosine, is an important reversible modification controlling protein activity. The conserved amino-terminal domains of the four core histones (H2A, H2B, H3, and H4) contain lysines that are acetylated by histone acetyltransferases (HATs) and deacetylated by histone deacetylases (HDACs) (1). Signaling resulting in acetylation/deacetylation of histones, transcription factors, and other proteins affects a diverse array of cellular processes including chromatin structure and gene activity, cell growth, differentiation, and apoptosis (2-6). Recent proteomic surveys suggest that acetylation of lysine residues may be a widespread and important form of posttranslational protein modification that affects thousands of proteins involved in control of cell cycle and metabolism, longevity, actin polymerization, and nuclear transport (7,8). The regulation of protein acetylation status is impaired in cancer and polyglutamine diseases (9), and HDACs have become promising targets for anti-cancer drugs currently in development (10).

赖氨酸的乙酰化如同丝氨酸、苏氨酸和酪氨酸的磷酸化一样,是一种控制蛋白活性的重要可逆手段。四个核心组蛋白(H2A, H2B, H3, H4)的保守氨基末端区域均包含赖氨酸,可以被组蛋白乙酰转移酶(HATs)乙酰化和组蛋白去乙酰酶(HDACs)去乙酰化(1)。组蛋白、转录因子和其它蛋白的乙酰化/去乙酰化信号最终影响多个细胞过程,包括染色体结构、基因活性、细胞生长、分化和凋亡等(2-6)。最近的蛋白组学研究显示赖氨酸残基的乙酰化是蛋白翻译后修饰的广泛而重要的手段,可以影响控制细胞周期、代谢、生存、肌动蛋白聚合和核转运相关的数千种蛋白。蛋白质乙酰化调节障碍在癌症和多聚谷氨酰胺疾病中发现(9),HDACs也成为目前正在开发的有希望的抗癌药物靶点(10)。

  1. Hassig, C.A. and Schreiber, S.L. (1997) Curr Opin Chem Biol 1, 300-8.
  2. Allfrey, V.G. et al. (1964) Proc Natl Acad Sci USA 51, 786-94.
  3. Liu, L. et al. (1999) Mol Cell Biol 19, 1202-9.
  4. Boyes, J. et al. (1998) Nature 396, 594-8.
  5. Polevoda, B. and Sherman, F. (2002) Genome Biol 3, reviews 0006.
  6. Yoshida, M. et al. (2003) Prog Cell Cycle Res 5, 269-78.
  7. Kim, S.C. et al. (2006) Mol Cell 23, 607-18.
  8. Choudhary, C. et al. (2009) Science 325, 834-40.
  9. Hughes, R.E. (2002) Curr Biol 12, R141-3.
  10. Vigushin, D.M. and Coombes, R.C. (2004) Curr Cancer Drug Targets 4, 205-18.

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For Research Use Only. Not For Use In Diagnostic Procedures.

U.S. Patent No. 5,675,063.

Use of Cell Signaling Technology (CST) Motif Antibodies within certain methods (e.g., U.S. Patents No. 7,198,896 and 7,300,753) may require a license from CST. For information regarding academic licensing terms please have your technology transfer office contact CST Legal Department at CST_ip@cellsignal.com. For information regarding commercial licensing terms please contact CST Pharma Services Department at ptmscan@cellsignal.com.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

MultiMab is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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