Cell Signaling Technology

Product Pathways - Cell Cycle / Checkpoint

Paclitaxel #9807

B-tubulin   microtubule   mitosis   taxane   taxol  

Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype

Species cross-reactivity is determined by western blot.

Applications Key:

Structure

Structure

Structure of Paclitaxel Paclitaxel的结构

Western Blotting

Western Blotting

Western blot analysis of extracts from HT29 cells, either untreated or treated with indicated concentration of paclitaxel using Phospho-Cdc25C (Thr48) Antibody #9527 (upper) or control Cdc25C (5H9) Rabbit mAb Antibody #4688 (lower).Western blot 方法检测HT-29细胞提取物,分非处理组和 paclitaxel 不同浓度处理组,使用的抗体为Phospho-Cdc25C (Thr48) Antibody #9527 (上图) 或 control Cdc25C (5H9) Rabbit mAb Antibody #4688 (下图)。

Directions for Use

Paclitaxel is supplied as 1 mg powder. Store at or below -20ºC. Before use, dissolve powder in 1.15 ml DMSO to make a 1 mM paclitaxel stock solution. For working concentrations of 100 nM-1000 nM, dilute DMSO stock 1:10,000 to 1:1000. Treat cells with the desired concentration for 6-48 hours. Paclitaxel is more stable as a powder than in solution; it is therefore recommended to dissolve as close as possible prior to use. Store solution at or below -20ºC.Paclitaxel为1mg粉末。-20ºC或更低温度保存。使用前用1.15 ml DMSO稀释溶解至1 mM贮存液。工作浓度为100 nM-1000 nM,将稀释DMSO贮存液1:10,000 到 1:1000。用需要的浓度处理细胞6-48小时。Paclitaxel粉末形式比液体更稳定,因此推荐使用前溶解至需要浓度。贮存于-20ºC或更低温度。

Background

Paclitaxel belongs to the taxane family of antitumor and antileukemic agents (3). By binding to β-tubulin and promoting the assembly of microtubules, paclitaxel prevents microtubual depolymerization and blocks normal cell division (1-3). The microtubule dysfunction induced by paclitaxel results in aberrant cell mitosis and sometimes apoptosis. The IC50 of paclitaxel-induced mitotic block is 4 nM (4). 紫杉醇属于抗肿瘤和抗白血病药物紫杉烷家族(3)。通过结合β-微管蛋白和促进微管装配,紫杉醇可以防止微管解聚,并阻止正常细胞分裂(1-3)。紫杉醇引起的微管功能障碍能够导致异常的细胞有丝分裂和偶发的凋亡。紫杉醇诱导有丝分裂阻遏的IC50是4nM (4)。

  1. Rowinsky, E.K. et al. (1990) J. Natl. Cancer Inst. 82, 1247-1259.
  2. Jordan, A. et al. (1998) Med. Res. Rev. 18, 259-296.
  3. Abal, M. et al. (2003) Curr. Cancer Drug Targets 3, 193-203.
  4. Long, B.H. and Fairchild, C.R. (1994) Cancer Res. 54, 4355-4361.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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