Cell Signaling Technology

Product Pathways - Stem Cell and Lineage Markers

RUNX2 (D1L7F) Rabbit mAb (PE Conjugate) #98059

No. Size Price
98059S 100 µl ( 50 tests ) ¥3,986.00 现货查询 购买询价
98059 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
F 1:50 Human,Mouse,Rat, Endogenous Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: F=Flow Cytometry,

Specificity / Sensitivity

RUNX2 (D1L7F) Rabbit mAb (PE Conjugate) recognizes endogenous levels of total RUNX2 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala273 of human RUNX2 protein.

Description

This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometry analysis in human cells. This antibody is expected to exhibit the same species cross-reactivity as the unconjugated RUNX2 (D1L7F) Rabbit mAb #12556.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of LNCaP (blue) and Saos-2 (green) cells using RUNX2 (D1L7F) Rabbit mAb (PE Conjugate).

Background

Runt-related transcription factor 2 (RUNX2) is a member of the RUNX family of transcription factors. It is involved in osteoblast differentiation and skeletal morphogenesis. RUNX2 regulates the transcription of various genes, including osteopontin, bone sialoprotein, and osteocalcin, via binding to the core site of the enhancers or promoters (1-3). RUNX2 is crucial for the maturation of osteoblasts and both intramembranous and endochondral ossification. Mutations in the corresponding RUNX2 gene have been associated with the bone development disorder cleidocranial dysplasia (CCD) (4-6). RUNX2 is also abnormally expressed in various human cancers including prostate cancer and breast cancer. It plays an important role in migration, invasion, and bone metastasis of prostate and breast cancer cells (7-10).

  1. Viereck, V. et al. (2002) J Cell Biochem 86, 348-56.
  2. Willis, D.M. et al. (2002) J Biol Chem 277, 37280-91.
  3. Tu, Q. et al. (2008) J Cell Physiol 217, 40-7.
  4. Quack, I. et al. (1999) Am J Hum Genet 65, 1268-78.
  5. Cardoso, B.M. et al. (2010) Clin Dysmorphol 19, 150-2.
  6. Han, M.S. et al. (2010) J Cell Biochem 110, 97-103.
  7. Akech, J. et al. (2010) Oncogene 29, 811-21.
  8. van der Deen, M. et al. (2010) J Cell Biochem 109, 828-37.
  9. Barnes, G.L. et al. (2003) Cancer Res 63, 2631-7.
  10. Barnes, G.L. et al. (2004) Cancer Res 64, 4506-13.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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