Cell Signaling Technology

Product Pathways - Growth Factors/Cytokines

SDF1 (D8G6H) Rabbit mAb (IHC-Specific) #97958

sc-74271  

No. Size Price
97958S 100 µl ( 200 sections ) ¥3,100.00 现货查询 购买询价
97958 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
IHC-P 1:200 Human, Endogenous 10 Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: IHC-P=Immunohistochemistry (Paraffin),

Specificity / Sensitivity

SDF1 (D8G6H) Rabbit mAb recognizes transfected and endogenous levels of total SDF1 protein by Western blot and immunohistochemistry on paraffin-embedded tissue sections, respectively. This antibody recognizes both SDF1α and SDF1β.

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant protein specific to human SDF1 protein.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma using SDF1 (D8G6H) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with Myc/DDK-tagged full-length human SDF1 protein (+), using SDF1 (D8G6H) Rabbit mAb (upper), DYKDDDDK Tag Antibody #2368 (middle), and β-Actin (D6A8) Rabbit mAb #8457 (lower).

Western Blotting

Western Blotting

Western blot analysis of recombinant human Human Stromal Cell-derived Factor 1β/CXCL12 (hSDF1β) #5712 (indicated amounts) using SDF1 (D8G6H) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using SDF1 (D8G6H) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using SDF1 (D8G6H) Rabbit mAb.

Background

The stromal cell derived factor 1 (SDF1, CXCL12) is a small, pro-inflammatory chemoattractant cytokine that regulates leukocyte trafficking through interactions with its cognate 7-transmembrane G protein-coupled receptors (1). The SDF1 receptor, CXCR4, also serves as a coreceptor for the entry of human immunodeficiency virus into target cells (2). SDF1 may regulate homing and maintenance of CXCR4-expressing stem or progenitor cells, including embryonic and many somatic stem cells (3,4). Many cancer cells express CXCR4, suggesting that SDF1 plays a role in cancer metastasis (5,6). Alternative splicing and differential processing during maturation produce a pair of SDF1 isoforms (SDF1α and SDF1β) that have different properties and biological activities (7). Additional isoforms of SDF1 have been reported (8,9).

  1. Maekawa, T. and Ishii, T. (2000) Intern Med 39, 90-100.
  2. Ray, N. and Doms, R.W. (2006) Curr Top Microbiol Immunol 303, 97-120.
  3. Sugiyama, T. et al. (2006) Immunity 25, 977-88.
  4. Ara, T. et al. (2003) Immunity 19, 257-67.
  5. Kucia, M. et al. (2004) J Mol Histol 35, 233-45.
  6. Ratajczak, M.Z. et al. (2006) Leukemia 20, 1915-24.
  7. De La Luz Sierra, M. et al. (2004) Blood 103, 2452-9.
  8. Gleichmann, M. et al. (2000) Eur J Neurosci 12, 1857-66.
  9. Yu, L. et al. (2006) Gene 374, 174-9.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Protocols

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

SignalStain is a trademark of Cell Signaling Technology, Inc.

Tween is a registered trademark of ICI Americas, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

用户评论 --- 共 0

该产品暂无评论!

我要参与评论 :

如要参与评论请先登录网站

还没有网站账户?去注册一下吧

Products

 

Applications