Cell Signaling Technology

Product Pathways - Lymphocyte Signaling

CXCR4 (D4Z7W) Rabbit mAb #97680

No. Size Price
97680S 100 µl ( 50 tests ) ¥3,100.00 现货查询 购买询价
97680 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
IHC-P 1:800 Human, Endogenous Rabbit IgG
F 1:50

Species cross-reactivity is determined by western blot.

Applications Key: IHC-P=Immunohistochemistry (Paraffin), F=Flow Cytometry,

Specificity / Sensitivity

CXCR4 (D4Z7W) Rabbit mAb recognizes endogenous levels of total CXCR4 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the extracellular domains of human CXCR4 protein.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of human peripheral blood mononucler cells using CXCR4 (D4Z7W) Rabbit mAb (right) co-stained with anti-human CD3-APC antibody compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (left). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of K-562 cells (blue) and Daudi cells (green) using CXCR4 (D4Z7W) Rabbit mAb. Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human Crohn's disease of the colon using CXCR4 (D4Z7W) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human tonsil using CXCR4 (D4Z7W) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded Jurkat (left) and 293T (right) cell pellets using CXCR4 (D4Z7W) Rabbit mAb.

Background

CXCR4 is a chemokine receptor that belongs to the G protein-coupled receptor family. It is activated by a small cytokine, CXCL12, also known as stromal cell derived factor 1 (SDF-1) (1). The main function of CXCR4 is the mediation of the homing of progenitor cells in the bone marrow and their recruitment to sites of injury (2). More recently, CXCR4 has been studied, as a potential therapeutic target, in the context of autoimmune diseases (3) as well as cancer, as the receptor is involved in the regulation of migration, proliferation, and survival of cancer cells (4).

  1. Blanchet, X. et al. (2012) Front Immunol 3, 175.
  2. Döring, Y. et al. (2014) Front Physiol 5, 212.
  3. Debnath, B. et al. (2013) Theranostics 3, 47-75.
  4. Teicher, B.A. and Fricker, S.P. (2010) Clin Cancer Res 16, 2927-31.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Protocols

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

SignalStain is a trademark of Cell Signaling Technology, Inc.

Alexa Fluor is a registered trademark of Life Technologies Corporation.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

用户评论 --- 共 0

该产品暂无评论!

我要参与评论 :

如要参与评论请先登录网站

还没有网站账户?去注册一下吧

Products

 

Applications