Cell Signaling Technology

Product Pathways - Growth Factors/Cytokines

TSLP (D7U1X) Rabbit mAb #97630

No. Size Price
97630S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
97630 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 19, 22 Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

TSLP (D7U1X) Rabbit mAb recognizes endogenous levels of total TSLP protein. This antibody recognizes a 70 kDa protein of unknown identity.

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant human TSLP protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from NHBE (normal human bronchial epithelial cells), untransfected (-) or transfected with poly(I:C) (10 μg/ml, 7 hr; +), using TSLP (D7U1X) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). Brefeldin A #9972 was added to cells at 300 ng/ml one hour after the start of the transfection.

Western Blotting

Western Blotting

Western blot analysis of recombinant human TSLP protein (hTSLP) using TSLP (D7U1X) Rabbit mAb.


TSLP (thymic stromal lymphopoietin) is a cytokine produced by epithelial cells at barrier surfaces and granulocytes (1). TSLP acts on dendritic cells, lymphocytes, and granulocytes by binding to a heterodimer composed of TSLPR (TSLP receptor) and IL-7Rα (2-5). Constitutive expression of TSLP at barrier surfaces contributes to homeostasis by promoting generation of noninflammatory dendritic cells (6). TSLP promotes the differentiation of Th2 cells and type 2 immune responses both in response to helminth infection and in the context of atopic diseases including asthma and atopic dermatitis (7-13).

  1. Soumelis, V. et al. (2002) Nat Immunol 3, 673-80.
  2. Reche, P.A. et al. (2001) J Immunol 167, 336-43.
  3. Levin, S.D. et al. (1999) J Immunol 162, 677-83.
  4. Park, L.S. et al. (2000) J Exp Med 192, 659-70.
  5. Pandey, A. et al. (2000) Nat Immunol 1, 59-64.
  6. Iliev, I.D. et al. (2007) J Exp Med 204, 2253-7.
  7. Taylor, B.C. et al. (2009) J Exp Med 206, 655-67.
  8. Ramalingam, T.R. et al. (2009) J Immunol 182, 6452-9.
  9. Ying, S. et al. (2005) J Immunol 174, 8183-90.
  10. Yoo, J. et al. (2005) J Exp Med 202, 541-9.
  11. Zhou, B. et al. (2005) Nat Immunol 6, 1047-53.
  12. Al-Shami, A. et al. (2005) J Exp Med 202, 829-39.

Application References

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