Cell Signaling Technology

Product Pathways - Apoptosis

Cleaved Caspase-6 (Asp162) Antibody #9761

Asp 162   Asp 179   Asp162   Asp179   casp367   caspase 6   caspase6   Mch2  

No. Size Price
9761S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
9761T 20 µl ( 2 western blots ) ¥1,300.00 现货查询 购买询价
9761 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 18 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

Cleaved Caspase-6 (Asp162) Antibody detects endogenous levels of the large subunit of caspase-6 resulting from cleavage at aspartic acid162. This antibody does not recognize other cleaved caspases.


Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the carboxy-terminal sequence of p18, the large subunit of rat caspase-6. Antibodies are purified by protein A and peptide affinity chromatography.

该多克隆抗体是采用合成的大鼠caspas-6 的大亚单位P18的C-末端序列相应的肽段免疫动物而生产制备的。利用蛋白A和肽亲和层析技术进行纯化。

Western Blotting

Western Blotting

Western blot analysis of Jurkat cell extracts, untreated or etoposide-treated, and NIH/3T3 and C6 cell extracts, untreated or staurosporine-treated, using Cleaved Caspase-6 (Asp162) Antibody.

对未处理或 etoposide 处理的Jurkat细胞,未处理或 staurosporine 处理的NIH/3T3 和 C6 细胞进行western Blot分析,抗体采用Cleaved Caspase-6 (Asp162) Antibody。


Caspase-6 (Mch2) is one of the major executioner caspases functioning in cellular apoptotic processes (1,2). Upon apoptotic stimulation, initiator caspases such as caspase-9 are cleaved and activated (3). The activated upstream caspases further process downstream executioner caspases, such as caspase-3 and caspase-6, by cleaving them into large and small subunits, thereby initiating a caspase cascade leading to apoptosis (4,5). One of the major targets for caspase-6 is the membrane associated protein lamin A (6). The cleavage of this protein causes cell membrane malfunction, membrane blebbing and eventual cell death.

Caspase-6 (Mch2) 是一种在细胞凋亡过程中发挥主要作用的效应酶(1.2)。一旦接收凋亡信号刺激,caspases启动酶如caspase-9 等被裂解而活化(3)。上游活化的caspases 进一步裂解下游caspases效应酶如caspase-3 和caspase-6, 产生大小亚单位,使之活化,进而启动caspase级联反应导致凋亡(4.5)。 膜相关蛋白lamin A 是caspase-6的主要靶蛋白之一(6)。caspase-6的裂解导致细胞膜功能障碍,形成膜泡,最终导致细胞死亡。

  1. Cohen, G.M. (1997) Biochem J 326 ( Pt 1), 1-16.
  2. Faleiro, L. et al. (1997) EMBO J 16, 2271-81.
  3. Li, P. et al. (1997) Cell 91, 479-89.
  4. Slee, E.A. et al. (1999) J Cell Biol 144, 281-92.
  5. MacFarlane, M. et al. (1997) J Cell Biol 137, 469-79.
  6. Orth, K. et al. (1996) J Biol Chem 271, 16443-6.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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