Product Pathways - Vesicle Trafficking
Golgin-97 (CDF4) Mouse mAb #97537
|97537S||100 µl ( 10 western blots )||￥3,250.00 现货查询||购买询价|
|97537||carrier free & custom formulation / quantity||email request|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IF-IC=Immunofluorescence (Immunocytochemistry),
Specificity / Sensitivity
Golgin-97 (CDF4) Mouse mAb recognizes endogenous levels of total golgin-97 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant human Golgin-97 protein.
Western blot analysis of extracts from various cell lines using Golgin-97 (CDF4) mouse mAb.
The Golgi-associated protein golgin A1 (GOLGA1, golgin-97) was first isolated as a Golgi complex autoantigen associated with the autoimmune disorder Sjogren's syndrome (1). The golgin-97 protein contains a carboxy-terminal GRIP domain and is a commonly used trans-Golgi network (TGN) marker. All four known mammalian GRIP domain-containing proteins (golgin-97, golgin-245, GCC88 and GCC185) are found in the TGN, share extensive alpha-helical structure, and form homodimers (2). While all four golgin proteins localize to the TGN, they exhibit different membrane-binding abilities and are found in distinct TGN regions (3). Golgin-97 and golgin-245 are targeted to the trans-Golgi network through an interaction between their GRIP domains and the Arl1 protein switch II region (4). Overexpression studies and siRNA assays with GRIP domain-containing proteins suggest that these proteins help to maintain trans-Golgi network integrity and function by controlling localization of TGN resident proteins (5). By using a Shiga toxin B fragment (STxB)-based in vitro transport assay and an E-cadherin transport model system, golgin-97 and its effector Arl1-GTP were shown to play a role in trans-Golgi endosomal trafficking (6,7). Research studies also suggest that golgin-97 may play a role in poxvirus morphogenesis and maturation (8,9).
- Griffith, K.J. et al. (1997) Arthritis Rheum 40, 1693-702.
- Luke, M.R. et al. (2005) Biochem J 388, 835-41.
- Derby, M.C. et al. (2004) J Cell Sci 117, 5865-74.
- Lu, L. and Hong, W. (2003) Mol Biol Cell 14, 3767-81.
- Yoshino, A. et al. (2003) J Cell Sci 116, 4441-54.
- Lu, L. et al. (2004) Mol Biol Cell 15, 4426-43.
- Lock, J.G. et al. (2005) Traffic 6, 1142-56.
- Alzhanova, D. and Hruby, D.E. (2006) J Virol 80, 11520-7.
- Alzhanova, D. and Hruby, D.E. (2007) Virology 362, 421-7.
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
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