Cell Signaling Technology

Product Pathways - Apoptosis

Cleaved Caspase-8 (Asp384) (11G10) Mouse mAb #9748

casp810   caspase 8   caspase-10   FLICE   MACH   Mch5  

No. Size Price
9748S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
9748 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 10 Mouse IgG1

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

Cleaved Caspase-8 (Asp384) (11G10) Mouse mAb detects endogenous levels of the small fragment of caspase-8 resulting from cleavage at aspartic acid 384. The antibody does not cross-react with full length caspase-8.

该抗体能够检测内源性水平的Asp384位剪切的caspase-8小片段。其与全长 caspase-8不发生交叉反应。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the amino-terminal sequence of p10 of human caspase-8. Antibody is supplied in HEPES buffer with 50% glycerol and less than 0.02% sodium azide.

该单克隆抗体是采用合成的人源caspase-8 p10片段的氨基末端周围残基相对应的多肽免疫动物后制备获得。该抗体存在于含50% 甘油和低于0.02% 叠氮化钠的HEPES缓冲液中。

Western Blotting

Western Blotting

Western blot analysis of extracts from SKW6.4 cells, untreated or anti-Fas-treated (1 µg/ml), using Cleaved Caspase-8 (Asp384) (11G10) Mouse mAb (left) or Caspase-8 (1C12) Mouse mAb #9746 (right).

采用抗体 Cleaved Caspase-8 (Asp384) (11G10) Mouse mAb 鼠单抗(左) or Caspase-8 (1C12) Mouse mAb 鼠单抗#9746 (右)对未处理或抗Fas抗体 (1 µg/ml)处理的SKW6.4 细胞进行Western blot 分析

Background

Apoptosis induced through the CD95 receptor (Fas/APO-1) and tumor necrosis factor receptor 1 (TNFR1) activates caspase-8 and leads to the release of the caspase-8 active fragments, p18 and p10 (1-3). Activated caspase-8 cleaves and activates downstream effector caspases such as caspase-1, -3, -6, and -7. Caspase-3 ultimately elicits the morphological hallmarks of apoptosis, including DNA fragmentation and cell shrinkage.

CD95受体(Fas/APO-1)和肿瘤坏死因子受体1(TNFR1)诱导的凋亡激活了caspase-8,并导致caspase-8释放活化片段p18和p10(1-3)。活化的caspase-8 裂解并活化下游的效应caspases,如caspase-1、-3、-6和-7。Caspase-3最终引起凋亡的形态学特征变化,包括DNA的片段化和细胞固缩。

(This product is sold under license from Promega Corp., U.S. Patent No. 6,350,452.)该产品的销售获得了Promega Corp., U.S.许可,专利号为 Patent No. 6,350,452

  1. Muzio, M. et al. (1996) Cell 85, 817-27.
  2. Boldin, M.P. et al. (1996) Cell 85, 803-15.
  3. Fernandes-Alnemri, T. et al. (1996) Proc Natl Acad Sci U S A 93, 7464-9.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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