Cell Signaling Technology

Product Pathways - PI3K / Akt Signaling

YB1 (D2A11) Rabbit mAb #9744

No. Size Price
9744S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
9744 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 49 Rabbit IgG
IP 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Homology

Species predicted to react based on 100% sequence homology: Xenopus, Bovine,

Specificity / Sensitivity

YB1 (D2A11) Rabbit mAb recognizes endogenous levels of total YB1 protein.

YB1 (D2A11) Rabbit mAb兔单抗检测内源性的总YB1蛋白.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human YB1 protein.

该单克隆抗体是由人源YB1蛋白羧基端附近的合成肽段免疫动物而生产的。

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using YB1 (D2A11) Rabbit mAb.

Western blot 分析不同细胞系提取物,所用抗体为YB1 (D2A11) Rabbit mAb兔单抗。

Background

The Y-box binding protein 1 (YB1) belongs to a family of evolutionarily conserved, multifunctional Y-box proteins that bind single-stranded DNA and RNA and function as regulators of transcription, RNA metabolism, and protein synthesis (1). YB1 binds to Y-box sequences (TAACC) found in multiple gene promoters and can positively or negatively regulate transcription. YB1 activates genes associated with proliferation and cancer, such as cyclin A, cyclin B1, matrix metalloproteinase-2 (MMP-2), and the multi-drug resistance 1 (MDR1) gene (2-4). YB1 represses genes associated with cell death, including the Fas cell death-associated receptor and the p53 tumor suppressor gene (5-7). It also interacts with the RNA-splicing factor SRp30c and stabilizes interleukin-2 (IL-2) mRNA upon induction of T lymphocytes by IL-2 (8,9). The majority of YB1 protein localizes to the cytoplasm, with a minor pool found in the nucleus; however, nuclear localization appears to be critical for its role in promoting proliferation. Nuclear translocation is cell cycle regulated, with YB1 protein accumulating in the nucleus during G1/S phase (2). In addition, nuclear translocation is induced in response to extracellular stimuli such as hyperthermia and UV irradiation, or treatment of cells with thrombin, interferons, or insulin-like growth factor (IGF-I) (2,10). Treatment of the MCF7 breast cancer cell line with IGF-I results in Akt-mediated phosphorylation of YB1 at Ser102, which is required for nuclear translocation of YB1 and its ability to promote anchorage-independent growth (10). Research studies have shown that YB1 is overexpressed in many malignant tissues, including breast cancer, non-small cell lung carcinoma, ovarian adenocarcinomas, human osteosarcomas, colorectal carcinomas, and malignant melanomas. Investigators have shown that nuclear YB1 expression correlates with high levels of proliferation, drug resistance, and poor tumor prognosis (2,7,10).

Y-box结合蛋白1(YB1)属于一类进化上保守的,多功能Y-box蛋白,和单链DNA和RNA结合,调控转录,RNA代谢和蛋白质的合成(1)。YB1与多种基因启动子中的Y-box序列(TAACC)结合,并能正向或负向调节转录。YB1激活与增殖和癌症相关的基因,如周期蛋白A,B1,基质金属蛋白酶-2(MMP-2),及多药耐药性1(MDR1)基因(2-4)。YB1抑制与细胞死亡有关的基因,包括细胞的Fas相关死亡受体和p53抑癌基因(5-7)。它还与RNA剪切因子SRp30c互作并且能够稳定诱导T淋巴细胞的白细胞介素2的mRNA(8,9)。YB1蛋白主要定位于细胞质,少量存在于细胞核中;然而,核里面的YB1对促进细胞增殖十分关键。核转运受到细胞周期调控,在G1 / S期YB1蛋白质积累在细胞核中(2)。此外,细胞受到外部刺激,如高温和紫外线照射,或着用凝血酶,干扰素,胰岛素样生长因子(IGF-1)处理后可以诱导发生核转运(2,10)。IGF-1处理MCF7乳腺癌细胞株诱发Akt介导的YB1(Ser102)磷酸化,促进YB1的核转运及非贴壁依赖性生长(10)。YB1在许多恶性肿瘤组织中高表达,包括乳腺癌,非小细胞肺癌,卵巢癌,人骨肉瘤,大肠癌,恶性黑色素瘤。核YB1表达与过度增殖,耐药性,及肿瘤的不良预后相关(2,7,10)。

  1. Matsumoto, K. and Wolffe, A.P. (1998) Trends Cell Biol. 8, 318-23.
  2. Jurchott, K. et al. (2003) J. Biol. Chem. 278, 27988-96.
  3. Mertens, P.R. et al. (1997) J. Biol. Chem. 272, 22905-12.
  4. Uchiumi, T. et al. (1993) Cell Growth Differ. 4, 147-57.
  5. Lasham, A. et al. (2000) Gene 252, 1-13.
  6. Lasham, A. et al. (2003) J. Biol. Chem. 278, 35516-23.
  7. Homer, C. et al. (2005) Oncogene 24, 8314-25.
  8. Raffetseder, U. et al. (2003) J. Biol. Chem. 278, 18241-8.
  9. Chen, C.Y. et al. (2000) Genes Dev. 14, 1236-48.
  10. Sutherland, B.W. et al. (2005) Oncogene 24, 4281-92.

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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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