Cell Signaling Technology

Product Pathways - Protein Translation

Phospho-eIF4E (Ser209) Antibody #9741

eif-4   eif4-epsilon   elf4   elf4e   Eukaryotic initiation factor 4E   sc-12885  

No. Size Price
9741L 300 µl ( 30 western blots ) ¥8,792.00 现货查询 购买询价
9741S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
9741T 20 µl ( 2 western blots ) ¥1,500.00 现货查询 购买询价
9741 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 25 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

Phospho-eIF4E (Ser209) Antibody detects endogenous levels of eIF4E only when phosphorylated at serine 209.

Phospho-eIF4E (Ser209) Antibody检测仅在serine 209位点磷酸化的内源性eIF4E蛋白水平。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser209 of human eIF4E. Antibodies are purified by protein A and peptide affinity chromatography.

通过人工合成人源eIF4E蛋白Ser209位点周围序列相应的磷酸化片段去免疫动物从而制备多克隆抗体。通过蛋白A和多肽亲和层析纯化抗体。

Western Blotting

Western Blotting

Western blot analysis of extracts from NIH/3T3 cells, untreated or treated with serum, PD98059 or Dexamethasone, using Phospho-eIF4E (Ser209) Antibody (upper) or eIF4E Antibody #9742 (lower).

使用Phospho-eIF4E (Ser209) Antibody (上图)和eIF4E Antibody #9742 (下图),免疫印迹(Western Blot)分析在NIH/3T3细胞系中Phospho-eIF4E (Ser209)和eIF4E蛋白水平,细胞分为对照组,PD98059处理组和Dexamethasone处理组。

Background

Eukaryotic initiation factor 4E (eIF4E) binds to the mRNA cap structure to mediate the initiation of translation (1,2). eIF4E interacts with eIF4G, a scaffold protein that promotes assembly of eIF4E and eIF4A into the eIF4F complex (2). eIF4B is thought to assist the eIF4F complex in translation initiation. Upon activation by mitogenic and/or stress stimuli mediated by Erk and p38 MAPK, Mnk1 phosphorylates eIF4E at Ser209 in vivo (3,4). Two Erk and p38 MAPK phosphorylation sites in mouse Mnk1 (Thr197 and Thr202) are essential for Mnk1 kinase activity (3). The carboxy-terminal region of eIF4G also contains serum-stimulated phosphorylation sites, including Ser1108, Ser1148, and Ser1192 (5). Phosphorylation at these sites is blocked by the PI3 kinase inhibitor LY294002 and by the FRAP/mTOR inhibitor rapamycin.

Eukaryotic initiation factor 4E (eIF4E)结合mRNA帽子结构从而介导翻译的起始(1,2)。eIF4E和eIF4G相互作用,而eIF4G是一个支架蛋白其能促进eIF4E和eIF4A装配到eIF4F复合物上(2)。eIF4B被认为在翻译起始阶段有助于eIF4F复合物的形成。在有丝分裂和Erk、p38 MAPK介导的压力刺激下,体内Mnk1蛋白使eIF4E蛋白在Ser209位点磷酸化(3,4)。在小鼠Mnk1上的两个Erk和p38 MAPK磷酸化位点(Thr197 and Thr202)对Mnk1激酶活性至关重要(3)。eIF4G蛋白羧基末端含有血清刺激的磷酸化位点,包括Ser1108、Ser1148和Ser1192 (5)。通过PI3激酶抑制剂LY294002和FRAP/mTOR通路抑制剂rapamycin可阻断这些位点的磷酸化。

  1. Sonenberg, N. et al. (1978) Proc. Natl. Acad. Sci. USA 75, 4843-4847.
  2. Gingras, A.C. et al. (1999) Annu. Rev. Biochem. 68, 913-963.
  3. Waskiewicz, A. et al. (1999) Mol. Cell. Biol. 19, 1871-1880.
  4. Pyronnet, S. et al. (1999) EMBO J. 18, 270-279.
  5. Raught, B. et al. (2000) EMBO J. 19, 434-444.

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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