Cell Signaling Technology

Product Pathways - Tyrosine Kinase / Adaptors

FGF Receptor 1 (D8E4) XP® Rabbit mAb #9740

fgf   fgfr   flg  

No. Size Price
9740S 100 µl ( 10 western blots ) ¥3,580.00 现货查询 购买询价
9740T 20 µl ( 2 western blots ) ¥1,400.00 现货查询 购买询价
9740 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 92 , 120, 145 Rabbit IgG
IP 1:50
IHC-P 1:500
F 1:400
IF-IC 1:200

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin), F=Flow Cytometry, IF-IC=Immunofluorescence (Immunocytochemistry),

Specificity / Sensitivity

FGF Receptor 1 (D8E4) XP® Rabbit mAb detects endogenous levels of total FGF receptor 1 protein. This antibody does not cross-react with other FGF receptor family members.

FGF Receptor 1 (D8E4) XP®兔单抗能够检测内源性水平的总FGF受体1蛋白。该抗体不与其他FGF受体家族成员发生交叉反应。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a recombinant protein specific to the carboxy terminus of human FGF receptor 1 protein.

该单克隆抗体是通过用重组蛋白免疫动物而制备的,该重组蛋白是针对人FGF受体1蛋白羧基末端。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using FGF Receptor 1 (D8E4) XP® Rabbit mAb.免疫组织化学方法检测石蜡包埋的人乳腺癌组织,使用的抗体为FGF Receptor 1 (D8E4) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human kidney using FGF Receptor 1 (D8E4) XP® Rabbit mAb.免疫组织化学方法检测石蜡包埋的人肾脏组织,使用的抗体为FGF Receptor 1 (D8E4) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using FGF Receptor 1 (D8E4) XP® Rabbit mAb.免疫组织化学方法检测石蜡包埋的人肺癌组织,使用的抗体为FGF Receptor 1 (D8E4) XP® Rabbit mAb.

IF-IC

IF-IC

Confocal immunofluorescent analysis of A204 cells (positive, left), KG-1 cells (positive, middle) and A172 cells (weak expression, right) using FGF Receptor 1 (D8E4) XP® Rabbit mAb (green). Blue pseudocolor= DRAQ5® #4084 (fluorescent DNA dye).激光共聚焦免疫荧光法检测A204细胞(阳性,左图),KG-1细胞(阳性,中图)和A172细胞(弱表达,右图),使用的抗体为FGF Receptor 1 (D8E4) XP® Rabbit mAb (绿色)。蓝色伪彩色为DNA荧光染料,产品信息为DRAQ5® #4084。

Western Blotting

Western Blotting

Western blot analysis of extracts from A-204 (FGFR1 positive), KG-1a (FGFR1 oncogenic partner-FGFR1 fusion), A172 (FGFR1 low), and HT-29 (FGFR1 negative) cells using FGF Receptor 1 (D8E4) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).Western blot方法检测A-204细胞(FGFR1阳性), KG-1a细胞 (FGFR1 致癌伴侣-FGFR1融合蛋白), A172 细胞(FGFR1低表达)和HT-29 细胞(FGFR1 阴性),使用的抗体为FGF Receptor 1 (D8E4) XP® Rabbit mAb (上图)和 β-Actin (D6A8) Rabbit mAb #8457 (下图).

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of A-204 cells using FGF Receptor 1 (D8E4) XP® Rabbit mAb (blue) compared to concentration matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (red).流式细胞术方法检测A-204细胞,使用的抗体为FGF Receptor 1 (D8E4) XP® Rabbit mAb (蓝色),与浓度相当的Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (红色)相比。

Background

Fibroblast growth factors (FGFs) produce mitogenic and angiogenic effects in target cells by signaling through cell surface receptor tyrosine kinases. There are four members of the FGF receptor family: FGFR1 (flg), FGFR2 (bek, KGFR), FGFR3, and FGFR4. Each receptor contains an extracellular ligand binding domain, a transmembrane domain, and a cytoplasmic kinase domain (1). Following ligand binding and dimerization, the receptors are phosphorylated at specific tyrosine residues (2). Seven tyrosine residues in the cytoplasmic tail of FGFR1 can be phosphorylated: Tyr463, 583, 585, 653, 654, 730, and 766. Tyr653 and Tyr654 are important for catalytic activity of activated FGFR and are essential for signaling (3). The other phosphorylated tyrosine residues may provide docking sites for downstream signaling components such as Crk and PLCγ (4,5).

成纤维细胞生长因子 (FGFs)通过细胞表面的酪氨酸激酶受体传递信号,对靶细胞产生促有丝分裂和血管生成的效应。已知FGF受体家族有四个成员:FGFR-1 (flg), FGFR-2 (bek, KGFR), FGFR-3和FGFR-4。每个受体都有一个胞外配体结合区、跨膜区和一个胞内激酶区(1)。在结合配体和二聚化之后,受体的特定的酪氨酸位点发生磷酸化(2)。在FGFR-1的羧基端有7个酪氨酸位点能被磷酸化:Tyr463, 583, 585, 653, 654, 730和 766。Tyr653 和Tyr654对激活的FGFR的催化活性十分重要,对信号传导也是必要的(3)。其它的磷酸化酪氨酸位点能够为下游信号分子,如Crk 和PLCγ,提供结合位点(4,5)。

  1. Powers, C.J. et al. (2000) Endocr Relat Cancer 7, 165-97.
  2. Reilly, J.F. et al. (2000) J Biol Chem 275, 7771-8.
  3. Mohammadi, M. et al. (1996) Mol Cell Biol 16, 977-89.
  4. Mohammadi, M. et al. (1991) Mol Cell Biol 11, 5068-78.
  5. Larsson, H. et al. (1999) J Biol Chem 274, 25726-34.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

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