Cell Signaling Technology

Product Pathways - MAPK Signaling

Phospho-MEF2A (Ser408) Antibody #9737

MADS   MEF2   Myocyte-specific enhancer factor 2A   Serum response factor-like protein 1  

No. Size Price
9737S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
9737 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Transfected Only 54 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Homology

Species predicted to react based on 100% sequence homology: Mouse,

Specificity / Sensitivity

Phospho-MEF2A (Ser408) Antibody detects transfected levels of MEF2A only when phosphorylated at Ser408. The antibody has also been shown to detect phospho-MEF2A (Ser408) in granule neurons (see application reference).

Phospho-MEF2A (Ser408) Antibody兔多抗能检测丝氨酸(408位点)磷酸化MEF2A的转染水平。该抗体也能检测颗粒神经细胞中的phospho-MEF2A (Ser408) (详见应用参考)

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser408 of human MEF2A. Antibodies are purified by protein A and peptide affinity chromatography.

该多克隆抗体是通过用与人源MEF2A蛋白丝氨酸(408位点)周围残基相一致的合成磷酸化肽段免疫动物后获得的。该抗体经蛋白A和肽亲和层析纯化。

Western Blotting

Western Blotting

Western blot analysis of extracts from COS-7 cells transfected with wild-type GAL4-MEF2A or mutant GAL4-MEF2A (S408A), using Phospho-MEF2A (Ser408) Antibody (top) and MEF2A Antibody #9736 (bottom).western blot方法检测细胞提取物:转染野生型GAL4-MEF2A和突变体GAL4-MEF2A (S408A)的COS-7细胞,使用的抗体是Phospho-MEF2A (Ser408) Antibody (t上图) 和 MEF2A Antibody #9736 (下图)。

Background

MEF2A is a member of the MEF2 (myocyte enhancer factor 2) family of transcription factors. In mammals, four MEF2A-related genes (MEF2A, MEF2B, MEF2C and MEF2D) encode proteins which exhibit significant amino acid sequence similarity within their DNA binding domains and to a lesser extent throughout the remaining proteins (1). The MEF2 family members were originally described as muscle-specific DNA binding proteins that recognize MEF2 motifs found within the promoters of many muscle-specific genes (2,3). Phosphorylation of MEF2A at Thr312 and Thr319 within the transcription activation domain by p38 MAP kinase enhances MEF2A-MEF2D heterodimer-dependent gene expression (4). On the other hand, apoptotic stimuli (e.g. neurotoxic insult) result in CDK5-dependent phosphorylation of MEF2A at Ser408 within the activation domain, inhibiting MEF2A pro-survival function (5).

MEF2A是转录因子MEF2(肌细胞增强子) 家族的成员,在哺乳动物体内,四个MEF2 A相关基因(MEF2A, MEF2B, MEF2C和 MEF2D)编码的蛋白在它们的DNA结合结构域有显著地氨基酸序列相似性,而其他部分的蛋白相似性较低(1)。起初人们认为MEF2家族成员是肌肉特异性DNA结合蛋白,它能识别许多肌肉特异性基因启动子内的MEF2结构(2,3)。p38 MAP激酶磷酸化MEF2A蛋白转录激活区域的苏氨酸(312、319位点),从而增强了MEF2A-MEF2D异二聚体依赖的基因表达(4)。另一方面,凋亡刺激因子(如神经毒的入侵)导致CDK5依赖型MEF2A蛋白激活区域丝氨酸(408位点)的磷酸化,抑制了MEF2A的促存活效应(5)。

  1. Shore, P. et al. (1995) Eur. J. Biochem. 229, 1-13.
  2. Martin, J.F. et al. (1994) Mol. Cell. Biol. 14, 1647-1656.
  3. Yu, Y.T. et al. (1992) Genes Dev. 6, 1783-1798.
  4. Zhao, M. et al. (1999) Mol. Cell. Biol. 19, 21-30.
  5. Gong, X. et al. (2003) Neuron 38, 33-46.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

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