Cell Signaling Technology

Product Pathways - MAPK Signaling

Phospho-MEF2A (Ser408) Antibody #9737

MADS   MEF2   Myocyte-specific enhancer factor 2A   Serum response factor-like protein 1  

No. Size Price
9737S 100 µl ( 10 western blots ) ¥4,050.00 现货查询 购买询价 防伪查询
9737 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Transfected Only 54 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,


Species predicted to react based on 100% sequence homology: Mouse,

Specificity / Sensitivity

Phospho-MEF2A (Ser408) Antibody detects transfected levels of MEF2A only when phosphorylated at Ser408. The antibody has also been shown to detect phospho-MEF2A (Ser408) in granule neurons (see application reference).

Phospho-MEF2A (Ser408) Antibody兔多抗能检测丝氨酸(408位点)磷酸化MEF2A的转染水平。该抗体也能检测颗粒神经细胞中的phospho-MEF2A (Ser408) (详见应用参考)

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser408 of human MEF2A. Antibodies are purified by protein A and peptide affinity chromatography.


Western Blotting

Western Blotting

Western blot analysis of extracts from COS-7 cells transfected with wild-type GAL4-MEF2A or mutant GAL4-MEF2A (S408A), using Phospho-MEF2A (Ser408) Antibody (top) and MEF2A Antibody #9736 (bottom).western blot方法检测细胞提取物:转染野生型GAL4-MEF2A和突变体GAL4-MEF2A (S408A)的COS-7细胞,使用的抗体是Phospho-MEF2A (Ser408) Antibody (t上图) 和 MEF2A Antibody #9736 (下图)。


MEF2A is a member of the MEF2 (myocyte enhancer factor 2) family of transcription factors. In mammals, four MEF2A-related genes (MEF2A, MEF2B, MEF2C and MEF2D) encode proteins which exhibit significant amino acid sequence similarity within their DNA binding domains and to a lesser extent throughout the remaining proteins (1). The MEF2 family members were originally described as muscle-specific DNA binding proteins that recognize MEF2 motifs found within the promoters of many muscle-specific genes (2,3). Phosphorylation of MEF2A at Thr312 and Thr319 within the transcription activation domain by p38 MAP kinase enhances MEF2A-MEF2D heterodimer-dependent gene expression (4). On the other hand, apoptotic stimuli (e.g. neurotoxic insult) result in CDK5-dependent phosphorylation of MEF2A at Ser408 within the activation domain, inhibiting MEF2A pro-survival function (5).

MEF2A是转录因子MEF2(肌细胞增强子) 家族的成员,在哺乳动物体内,四个MEF2 A相关基因(MEF2A, MEF2B, MEF2C和 MEF2D)编码的蛋白在它们的DNA结合结构域有显著地氨基酸序列相似性,而其他部分的蛋白相似性较低(1)。起初人们认为MEF2家族成员是肌肉特异性DNA结合蛋白,它能识别许多肌肉特异性基因启动子内的MEF2结构(2,3)。p38 MAP激酶磷酸化MEF2A蛋白转录激活区域的苏氨酸(312、319位点),从而增强了MEF2A-MEF2D异二聚体依赖的基因表达(4)。另一方面,凋亡刺激因子(如神经毒的入侵)导致CDK5依赖型MEF2A蛋白激活区域丝氨酸(408位点)的磷酸化,抑制了MEF2A的促存活效应(5)。

  1. Shore, P. et al. (1995) Eur. J. Biochem. 229, 1-13.
  2. Martin, J.F. et al. (1994) Mol. Cell. Biol. 14, 1647-1656.
  3. Yu, Y.T. et al. (1992) Genes Dev. 6, 1783-1798.
  4. Zhao, M. et al. (1999) Mol. Cell. Biol. 19, 21-30.
  5. Gong, X. et al. (2003) Neuron 38, 33-46.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!


Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

用户评论 --- 共 0


我要参与评论 :