Cell Signaling Technology

Product Pathways - Apoptosis

Cleaved DFF45 (Asp224) Antibody #9731


No. Size Price
9731S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
9731 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 12 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

Cleaved DFF45 (Asp224) Antibody detects the carboxy-terminal fragment of DFF45 resulting from cleavage at aspartic acid 224. The antibody does not recognize full length DFF45 or other cleavage products of DFF45.

该抗体能够检测天冬氨酸224处裂解的DFF45羧基末端片段, 不能识别全长DFF45及其他DFF45裂解产物。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to carboxy-terminal residues surrounding Asp224 of human DFF45. Antibodies are purified by protein A and peptide affinity chromatography.

该多克隆抗体是用合成的人DFF45 224位天冬氨酸C-末端序列相对应的肽段免疫的动物而制备获得。抗体经过了蛋白A和肽亲和层析技术进行纯化。

Western Blotting

Western Blotting

Western blot analysis of extracts from Jurkat cells, untreated or etoposide-treated (25 µM), using Cleaved DFF45 (Asp224) Antibody. 采用Cleaved DFF45 (Asp224) Antibody对未处理与依托泊苷处理(25uM)的Jurkat细胞进行免疫印迹分析(western blot)分析。


Human DFF45 and its mouse homologue ICAD function in normal cells as chaperones for caspase-activated deoxyribonuclease (DFF40 or CAD) during its synthesis (1). The association of DFF45 (or its isoform DFF35) with DFF40 inhibits the DNAse activity of the latter (1-4). In vitro, DFF45 has been shown to be the target of several caspases, including caspase-3, -6, -7, -8 and granzyme B (3). In vivo, caspase-3 is believed to be the primary enzyme responsible for processing DFF45 and release of its carboxy-terminal fragment (3,5). The cleavage of DFF45 inactivates its inhibitory function on DFF40 and causes nuclear DNA degradation by DFF40, leading to cell death (6,7).

在正常细胞中,在 caspase 激活的脱氧核苷酸酶( (DFF40 or CAD) 合成过程中,人源DFF45和小鼠同源的ICAD作为分子伴侣共同发挥作用(1)。DFF45 (或同源的 DFF35)与DFF40联合抑制DFF40的活性(1-4)。体外试验中,研究证明 DFF45 包括caspase-3、-6、-7和-8等caspas家族和端粒酶B的靶点(3)。体内试验研究认为,caspas-3是主要的DFF45处理酶并释放其羧基末端片段(3,5)。DFF45的裂解阻止了其对DFF40的抑制作用,造成核DNA被DFF40裂解,最终导致细胞死亡(6,7).

(This product is sold under license from Promega Corp., U.S. Patent No. 6,350,452.)该产品通过了许可证,(Promega Corp,U.S. Patent No. 6,350,452)

  1. Enari, M. et al. (1998) Nature 391, 43-50.
  2. Sakahira, H. et al. (1998) Nature 391, 96-99.
  3. Wolf, B. B. et al. (1999) J. Biol. Chem. 274, 30651-30656.
  4. Gu, J. et al. (1999) J. Biol. Chem. 274, 20759-20762.
  5. Tang, D. and Kidd, V.J. (1998) J. Biol. Chem. 273, 28549-28552.
  6. Liu, X. et al. (1999) J. Biol. Chem. 274, 13836-13840.
  7. Zhang, J. et al. (1999) J. Biol. Chem. 274, 37450-37454.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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