Cell Signaling Technology

Product Pathways - Protein Translation

Phospho-eIF2α (Ser51) Antibody #9721

eif   eif-2   eif2-alpha   eif2a   eif2alpha   elf-2   elf2  

No. Size Price
9721L 300 µl ( 30 western blots ) ¥8,792.00 现货查询 购买询价
9721S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
9721 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey,D. melanogaster, Endogenous 38 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

Phospho-eIF2alpha (Ser51) Antibody detects endogenous eIF2alpha only when phosphorylated at Ser51. The antibody does not recognize elF2alpha phosphorylated at other sites.

Phospho-eIF2alpha (Ser51) Antibody检测仅在Ser51位点磷酸化的内源性eIF2alpha蛋白水平。该抗体不与其它位点磷酸化的elF2alpha蛋白发生交叉反应。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser51 of human eIF2alpha. Antibodies are purified by protein A and peptide affinity chromatography.

通过人工合成人源eIF2alpha蛋白Ser51位点周围序列相应的磷酸化片段去免疫动物从而制备多克隆抗体。通过蛋白A和多肽亲和层析纯化抗体。

Western Blotting

Western Blotting

Western blot analysis of extracts from PC12 cells, untreated or thapsigargin-treated (300 nM), using Phospho-eIF2alpha (Ser51) Antibody (upper) or eIF2alpha Antibody #9722 (lower).

使用Phospho-eIF2alpha (Ser51) Antibody (上图)和eIF2alpha Antibody #9722 (下图),免疫印迹(Western Blot)分析在PC12细胞系中Phospho-eIF2α (Ser51)和eIF2alpha蛋白水平水平,细胞分为对照组和thapsigargin(300 nM)处理组。

Background

Phosphorylation of the eukaryotic initiation factor 2 (eIF2) α subunit is a well-documented mechanism to downregulate protein synthesis under a variety of stress conditions. Eukaryotic initiation factor 2 binds GTP and Met-tRNAi and transfers Met-tRNA to the 40S subunit to form the 43S preinitiation complex (1,2). eIF2 promotes a new round of translation initiation by exchanging GDP for GTP, a reaction catalyzed by eIF2B (1,2). Kinases that are activated by viral infection (PKR), endoplasmic reticulum stress (PERK/PEK), amino acid deprivation (GCN2), or heme deficiency (HRI) can phosphorylate the α subunit of eIF2 (3,4). This phosphorylation stabilizes the eIF2-GDP-eIF2B complex and inhibits the turnover of eIF2B. Induction of PKR by IFN-γ and TNF-α induces potent phosphorylation of eIF2α at Ser51 (5,6).

研究证明在不同的应激条件下真核生物起始因子2 (eIF2) α亚型的磷酸化可下调蛋白质的合成。eIF2蛋白与GTP以及起始tRNA(Met-tRNAi)结合,然后将Met-tRNA转移到40S核糖体亚基上共同形成43S前起始复合物(1,2)。eIF2通过eIF2B催化反应将GTP交换GDP从而促进新一轮翻译的起始(1,2)。病毒感染(PKR)、内质网应激(endoplasmic reticulum stress)(PERK/PEK)、氨基酸饥饿(GCN2)或血红素缺乏症(HRI)能够激活相关的激酶,这些激酶可以使eIF2的α亚基磷酸化(3,4)。这个磷酸化稳固eIF2-GDP-eIF2B复合物,同时期抑制eIF2B的降解。通过IFN-γ 和TNF-α的诱导的PKR能有效的诱导eIF2α蛋白在Ser51位点磷酸化(5,6)。

  1. Kimball, S.R. (1999) Int. J. Biochem. Cell Biol. 31, 25-29.
  2. De Haro, C. et al. (1996) FASEB J. 10, 1378-1387.
  3. Kaufman, R.J. (1999) Genes Dev. 13, 1211-1233.
  4. Sheikh, M.S. and Fornace Jr., A.J. (1999) Oncogene 18, 6121-6128.
  5. Cheshire, J.L. et al. (1999) J. Biol. Chem. 274, 4801-4806.
  6. Zamanian-Daryoush, M. et al. (2000) Mol. Cell. Biol. 20, 1278-1290.

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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