Cell Signaling Technology

Product Pathways - MAPK Signaling

Phospho-HSP27 (Ser82) (D1H2F6) XP® Rabbit mAb #9709

No. Size Price
9709S 100 µl ( 10 western blots ) ¥4,050.00 现货查询 购买询价
9709T 20 µl ( 2 western blots ) ¥1,600.00 现货查询 购买询价
9709 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse, Endogenous 27 Rabbit IgG
IHC-P 1:100
F 1:100
IF-IC 1:200

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IHC-P=Immunohistochemistry (Paraffin), F=Flow Cytometry, IF-IC=Immunofluorescence (Immunocytochemistry),

Homology

Species predicted to react based on 100% sequence homology: Rat, Hamster, Bovine, Dog, Horse,

Specificity / Sensitivity

Phospho-HSP27 (Ser82) (D1H2) XP® Rabbit mAb recognizes endogenous levels of HSP27 protein only when phosphorylated at Ser82.

Phospho-HSP27(Ser82)(D1H2)XP® Rabbit mAb兔单抗可识别内源性的Ser82磷酸化的HSP27。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser82 of human HSP27 protein.

该单克隆抗体用与人类HSP27蛋白中Ser82位点附近的氨基酸序列对应的人工合成磷酸化肽段免疫动物而制成。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded HeLa cell pellets, control (left) or UV-treated (right), using Phospho-HSP27 (Ser82) (D1H2) XP® Rabbit mAb.

对石蜡包埋的HeLa细胞沉淀进行免疫组化分析,分为对照(左)或UV处理(右),使用Phospho-HSP27 (Ser82) (D1H2) XP® Rabbit mAb兔单抗进行检测。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using Phospho-HSP27 (Ser82) (D1H2) XP® Rabbit mAb.

对石蜡包埋的人前列腺癌使用Phospho-HSP27 (Ser82) (D1H2) XP® Rabbit mAb兔单抗进行免疫组化分析。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, control (left) or λ phosphatase-treated (right), using Phospho-HSP27 (Ser82) (D1H2) XP® Rabbit mAb.

对石蜡包埋的人乳腺癌,对照(左)或用γ磷酸酶处理(右),使用Phospho-HSP27 (Ser82) (D1H2) XP® Rabbit mAb兔单抗进行免疫组化分析。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Phospho-HSP27 (Ser82) (D1H2) XP® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).

对石蜡包埋的人肺癌使用Phospho-HSP27 (Ser82) (D1H2) XP® Rabbit mAb兔单抗进行免疫组织化学分析,其中左图使用对照肽,右图使用抗原特异肽。

IF-IC

IF-IC

Confocal immunofluorescent analysis of C2C12 cells, untreated (left) or treated with λ phosphatase (middle), and NIH/3T3 cells (right) using Phospho-HSP27 (Ser82) (D1H2) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). Negative staining in NIH/3T3 cells is in agreement with the observation that NIH/3T3 cells do not express HSP27 under basal conditions (5,7).

对C2C12细胞,分为未处理(左)或λ磷酸酶处理(中),以及NIH/3T3细胞(右),使用Phospho-HSP27 (Ser82) (D1H2) XP® Rabbit mAb(绿色)进行共聚焦免疫荧光分析。肌动蛋白用DY-554 phalloidin(红色)标记。蓝色伪彩=DRAQ5®#4084(荧光DNA染料)。NIH/3T3细胞的阴性染色与观察到的基本条件下NIH/3T3细胞不表达HSP27的现象一致(5,7)。

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of HeLa cells, untreated (blue) or UV-treated (green), using Phospho-HSP27 (Ser82) (D1H2) XP® Rabbit mAb.

对未处理(蓝)或紫外线处理(绿)的Hela细胞使用Phospho-HSP27 (Ser82) (D1H2) XP® Rabbit mAb兔单抗进行流式细胞仪分析。

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa or HT-29 cells, untreated (-) or treated (+) with either UV (40 mJ/cm2 with 30 min recovery) or anisomycin (25 μg/mL, 30 min), using Phospho-HSP27 (Ser82) (D1H2) XP® Rabbit mAb.

对未处理(-)或者UV (40mJ/cm2,30分钟恢复)或anisomycin (25 ug/ml,30分钟)处理过的HeLa和HT-29细胞使用Phospho-HSP27 (Ser82) (D1H2) XP® Rabbit mAb兔单抗进行Western blot分析。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded mouse lung using Phospho-HSP27 (Ser82) (D1H2) XP® Rabbit mAb.

对石蜡包埋的小鼠肺部组织,使用Phospho-HSP27 (Ser82) (D1H2) XP® Rabbit mAb兔单抗进行免疫组化分析。

Background

Heat shock protein (HSP) 27 is one of the small HSPs that are constitutively expressed at different levels in various cell types and tissues. Like other small HSPs, HSP27 is regulated at both the transcriptional and posttranslational levels (1). In response to stress, the HSP27 expression increases several-fold to confer cellular resistance to the adverse environmental change. HSP27 is phosphorylated at Ser15, Ser78, and Ser82 by MAPKAPK-2 as a result of the activation of the p38 MAP kinase pathway (2,3). Phosphorylation of HSP27 causes a change in its tertiary structure, which shifts from large homotypic multimers to dimers and monomers (4). It has been shown that phosphorylation and increased concentration of HSP27 modulates actin polymerization and reorganization (5,6).

热休克蛋白(HSP)27是小HSP之一,在多种细胞和组织中都有不同水平的组成性表达。像其它小HSP一样,HSP27的调节也发生在转录和翻译后(1)。应激反应中,HSP27表达增加了数倍以实现细胞对不利环境的抵抗。HSP27被MAPKAPK-2在Ser15、Ser78和Ser82磷酸化,作为p38 MAP激酶途径活化的结果(2,3)。HSP27磷酸化导致其四级结构改变,以至于从大分子量的同型多聚体变为二聚体和单体(4)。目前显示HSP27磷酸化和浓度增加可以调节肌动蛋白多聚化和再组织(5,6)。

  1. Arrigo, A.P. and Landry, J. (1994) Cold Spring Harbor Laboratory Press, NY, 335-373.
  2. Landry, J. et al. (1992) J. Biol. Chem. 267, 794-803.
  3. Rouse, J. et al. (1994) Cell 78, 1027-1037.
  4. Rogalla, T. et al. (1999) J. Biol. Chem. 274, 18947-18956.
  5. Lavoie, J. et al. (1993) J. Biol. Chem. 268, 24210-24214.
  6. Rousseau, S. et al. (1997) Oncogene 15, 2169-2177.
  7. Aoyama, A. et al. (1993) Mol Cell Biol 13, 1824-35.

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For Research Use Only. Not For Use In Diagnostic Procedures.

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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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