Cell Signaling Technology

Product Pathways - Chromatin Regulation / Epigenetics

Phospho-Histone H3 (Ser10) (6G3) Mouse mAb #9706

No. Size Price
9706L 300 µl ( 30 western blots ) ¥8,792.00 现货查询 购买询价
9706S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
9706 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 17 Mouse IgG1
F 1:25
IF-F 1:100
IF-IC 1:400

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, F=Flow Cytometry, IF-F=Immunofluorescence (Frozen), IF-IC=Immunofluorescence (Immunocytochemistry),

Specificity / Sensitivity

Phospho-Histone H3 (Ser10) (6G3) Mouse mAb detects endogenous levels of histone H3 only when phosphorylated at serine 10. The antibody does not cross-react with other phosphorylated histones or acetylated histone H3.

Phospho-Histone H3 (Ser10) (6G3) Mouse mAb鼠单抗能够检测仅在serine 10位点磷酸化的内源性histone H3蛋白水平。该抗体不能与其它磷酸化的组蛋白或乙酰化的histone H3发生交叉反应。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser10 of human histone H3.

通过合成的与人源histone H3蛋白Ser10位点周围相应的磷酸化片段去免疫动物从而制备出此单克隆抗体。

Western Blotting

Western Blotting

Western blot analysis of whole cell lysates from NIH/3T3 cells, untreated or treated with serum plus calyculin A (to induce phosphorylation of H3), using Phospho-Histone H3 (Ser10) (6G3) Mouse mAb (top), Phospho-Histone H3 (Ser10) Antibody #9701 (middle) or Histone H3 Antibody #9712 (bottom).

使用Phospho-Histone H3 (Ser10) (6G3) Mouse mAb鼠单抗(上图)、Phospho-Histone H3 (Ser10) Antibody #9701 (中图)或Histone H3 Antibody #9712 (下图),免疫印迹(Western blot)分析NIH/3T3细胞,细胞分为未处理 或处理了serum plus calyculin A (来引起磷酸化的H3)。

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of Phospho-Histone H3 (Ser10) (6G3) Mouse mAb staining of untreated (blue) or serum/calyculin-treated (green) Ramos cells compared to a nonspecific negative control antibody (red).

与一个非特异阴性control antibody (红色)比较,使用Phospho-Histone H3 (Ser10) (6G3) Mouse mAb鼠单抗进行流式细胞仪分析untreated (蓝色)或serum/calyculin-treated (绿色) Ramos细胞。

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of pacilitaxel-treated THP1 cells, using Phospho-Histone H3 (Ser10) (6G3) Mouse mAb versus propidium iodide (DNA content). The red population indicates positive Phospho-Histone H3 cells.

使用Phospho-Histone H3 (Ser10) (6G3) Mouse mAb鼠单抗与propidium iodide (DNA 含量),流式细胞仪分析pacilitaxel-treated THP1细胞。红色群组是阳性的磷酸化Histone H3细胞。



Confocal immunofluorescent analysis of NIH/3T3 cells labeled with Phospho-Histone H3 (Ser10) (6G3) Mouse mAb (red) and alpha/beta-Tubulin Antibody #2148 (green) showing different stages of the cell cycle. Nonmitotic (A), prophase (B), metaphase (C) and anaphase (D). 使用Phospho-Histone H3 (Ser10) (6G3) Mouse mAb (红色)和alpha/beta-Tubulin Antibody #2148 (绿色)标记,共聚焦免疫荧光分析NIH/3T3细胞。结构显示细胞周期的不同的阶段。非有丝分裂(Nonmitotic,A)、前期(Prophase,B)、中期(metaphase,C)、后期(anaphase,D)。



Confocal immunofluorescent analysis of proliferating/mitotic cells labeled with Phospho-Histone H3 (Ser10) (6G3) Mouse mAb (blue) in the subventricular zone following 4 h reperfusion after cerebral ischemia. Red = EGR1 antibody #4152. Green = Phospho-S6 Ribosomal Protein (Ser235/236) (2F9) Rabbit mAb (Alexa Fluor® 488 Conjugate) #4854.

在脑缺血后在灌注4小时的室管膜下区,使用 Phospho-Histone H3 (Ser10) (6G3) Mouse mAb 鼠单抗(蓝色)标记,共聚焦免疫荧光分析增殖/有丝分裂细胞。红色=EGR1 antibody #4152。绿色=Phospho-S6 Ribosomal Protein (Ser235/236) (2F9) Rabbit mAb (Alexa Fluor® 488 Conjugate) #4854。


Modulation of chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin (1). The amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination (2-5). These modifications occur in response to various stimuli and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, gene expression (6). In most species, histone H2B is primarily acetylated at Lys5, 12, 15, and 20 (4,7). Histone H3 is primarily acetylated at Lys9, 14, 18, 23, 27, and 56. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (2,3). Phosphorylation at Ser10, Ser28, and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis (8-10). Phosphorylation at Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation at Thr3 of H3 in prophase and its dephosphorylation during anaphase (11).

染色质结构的修饰在调节真核细胞的转录中扮演着重要的角色。由DNA围绕和八聚体组蛋白(H2A,H2B,H3和H4各两个)共同组成的核小体是染色质的主要组成(1)。核小体的组蛋白氨基酸尾端进行不同的转录后修饰,包括乙酰化,磷酸化,甲基化和泛素化(2-5)。这些修饰通过不同的刺激产生并且对转录因子能否接近染色质有着直接的影响,所以也影响着基因的表达(6)。在大多数的物种中组蛋白H2B主要在Lys5,,12,,15和20位点上发生乙酰化(4,7)。组蛋白H3主要是在Lys9,14,18,23,27和56位点上发生乙酰化。在某些物种里H3上的Lys9位点发生乙酰化并应该在组蛋白沉积和染色质组装中扮演着重要的角色(2,3)。组蛋白H3上Ser10,Ser28和Thr11位点的磷酸化在有丝分裂和无丝分裂中都与染色质的缩合紧密相连(8-10)。H3的Thr3位点的磷酸化在许多物种中都是高度保守的,是由kinase haspin所催化的。在哺乳动物中用磷酸化特异性的抗体做免疫组化显示H3的Thr3位点在有丝分裂的前期发生磷酸化,后期发生去磷酸化(11)。

  1. Workman, J.L. and Kingston, R.E. (1998) Annu Rev Biochem 67, 545-79.
  2. Hansen, J.C. et al. (1998) Biochemistry 37, 17637-41.
  3. Strahl, B.D. and Allis, C.D. (2000) Nature 403, 41-5.
  4. Cheung, P. et al. (2000) Cell 103, 263-71.
  5. Bernstein, B.E. and Schreiber, S.L. (2002) Chem Biol 9, 1167-73.
  6. Jaskelioff, M. and Peterson, C.L. (2003) Nat Cell Biol 5, 395-9.
  7. Thorne, A.W. et al. (1990) Eur J Biochem 193, 701-13.
  8. Hendzel, M.J. et al. (1997) Chromosoma 106, 348-60.
  9. Goto, H. et al. (1999) J Biol Chem 274, 25543-9.
  10. Preuss, U. et al. (2003) Nucleic Acids Res 31, 878-85.
  11. Dai, J. et al. (2005) Genes Dev 19, 472-88.

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