Cell Signaling Technology

Product Pathways - Chromatin Regulation / Epigenetics

Phospho-Histone H3 (Ser10) Antibody #9701

No. Size Price
9701L 300 µl ( 30 western blots ) ¥8,792.00 现货查询 购买询价
9701S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
9701 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey,D. melanogaster,S. cerevisiae, Endogenous 17 Rabbit
IHC-P 1:200
F 1:50
IF-IC 1:200
IHC-F 1:400

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IHC-P=Immunohistochemistry (Paraffin), F=Flow Cytometry, IF-IC=Immunofluorescence (Immunocytochemistry), IHC-F=Immunohistochemistry (Frozen),

Homology

Species predicted to react based on 100% sequence homology: Xenopus,

Specificity / Sensitivity

Phospho-Histone H3 (Ser10) Antibody detects endogenous levels of histone H3 only when phosphorylated at serine 10. The antibody does not cross-react with other phosphorylated histones or with acetylated histones.

Phospho-Histone H3 (Ser10) Antibody检测仅在Ser10位点磷酸化的内源性histone H3的蛋白水平。该抗体不能与其他磷酸化的组蛋白或乙酰化的组蛋白发生交叉反应。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser10 of human histone H3. Antibodies are purified by protein A and peptide affinity chromatography.

通过合成的与人源histone H3蛋白Ser10位点周围相应的磷酸化片段去免疫动物从而制备出此多克隆抗体。通过蛋白A和多肽亲和层析纯化获得。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical staining of phosphorylated histone H3 in paraffin-embedded human breast carcinoma showing nuclear localization using Phospho-Histone H3 (Ser10) Antibody.

使用Phospho-Histone H3 (Ser10) Antibody,免疫组化分析人源乳腺癌石蜡切片中histone H3的磷酸化。

IC-ABC

IC-ABC

Immunocytochemical staining of NIH/3T3 cells, nocodazole-treated (2 ug/ml) and grown in 10% serum, showing cells undergoing mitosis using Phospho-Histone (Ser10) Antibody.

使用Phospho-Histone (Ser10) Antibody,免疫细胞化学染色NIH/3T3细胞,细胞经过nocodazole-treated (2 ug/ml)和10% serum培养。结果显示细胞正在经历有丝分裂。

Western Blotting

Western Blotting

Western blot analysis of whole cell lysates of NIH/3T3 cells, untreated, treated with TSA (to induce histone acetylation), serum plus calyculin A (to induce phosphorylation of H3) or both, using Phospho-Histone H3 (Ser10) Antibody.

使用Phospho-Histone H3 (Ser10) Antibody,免疫印迹(Western blot)分析NIH/3T3全细胞裂解液,其分为未处理、处理了TSA (为了诱导组蛋白乙酰化)、处理serum plus calyculin A (为诱导H3的磷酸化)或两者都处理。

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of Phospho-Histone H3 (Ser10) Antibody staining of untreated (blue) or serum/calyculin treated (green) Ramos cells compared to a nonspecific negative control antibody (red).

与非特异阴性control antibody (红色)比较,使用Phospho-Histone H3 (Ser10) Antibody,流式细胞仪分析未处理的untreated (蓝色)或serum/calyculin treated (绿色) Ramos细胞。

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of untreated Jurkat cells, using Phospho-Histone H3 (Ser10) Antibody versus propidium iodide (DNA content). The box indicates phospho-histone H3 positive cells.

使用Phospho-Histone H3 (Ser10) Antibody和propidium iodide (DNA含量),流式细胞仪分析未处理的Jurkat细胞。方框代表phospho-histone H3阳性细胞。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human tonsil using Phospho-Histone H3 (Ser10) Antibody.

使用Phospho-Histone H3 (Ser10) Antibody,免疫组化分析人源扁桃体组织石蜡切片。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, untreated (left) or lambda phosphatase-treated (right), using Phospho-Histone H3 (Ser10) Antibody.

使用Phospho-Histone H3 (Ser10) Antibody,免疫组化分析人源结肠癌组织石蜡切片,切片分为untreated (左图)或lambda phosphatase-treated (右图)。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of HT29 cells using Phospho-Histone H3 (Ser10) Antibody. Note the specific staining of mitotic cells.

使用Phospho-Histone H3 (Ser10) Antibody,免疫组化分析HT29细胞石蜡切片。说明有丝分裂细胞的特异性染色。

IF-IC

IF-IC

Confocal microscopic image of a mitotic HeLa cell labeled with Phospho-Histone H3 (Ser10) Antibody (red) and Survivin (6E4) Mouse mAb #2802 (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

使用Phospho-Histone H3 (Ser10) Antibody (红色)和Survivin (6E4) Mouse mAb #2802 (绿色)标记,共聚焦显微镜观察有丝分裂期HeLa细胞。蓝色= DRAQ5® #4084 (DNA荧光染料)。

IHC-F (frozen)

IHC-F (frozen)

Immunohistochemical analysis of frozen H1650 xenograft, showing staining of mitotic cells using Phospho-Histone H3 (Ser10) Antibody.

使用Phospho-Histone H3 (Ser10) Antibody,免疫组化分析H1650 xenograft冰冻切片,结果显示有丝分裂期细胞的染色。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Phospho-Histone H3 (Ser10) Antibody in the presence of control peptide (left) or Phospho-Histone H3 (Ser10) Blocking Peptide #1000 (right).

使用Phospho-Histone H3 (Ser10) Antibody,左图:control peptide或 右图:Phospho-Histone H3 (Ser10) Blocking Peptide #1000 ,免疫组化分析人源肺癌石蜡切片。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded 4T1 syngeneic mouse tumor using Phospho-Histone H3 (Ser10) Antibody #9701.

使用Phospho-Histone H3 (Ser10) Antibody,免疫组化分析4T1 syngeneic mouse tumor石蜡切片。

Background

Modulation of chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin (1). The amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination (2-5). These modifications occur in response to various stimuli and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, gene expression (6). In most species, histone H2B is primarily acetylated at Lys5, 12, 15, and 20 (4,7). Histone H3 is primarily acetylated at Lys9, 14, 18, 23, 27, and 56. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (2,3). Phosphorylation at Ser10, Ser28, and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis (8-10). Phosphorylation at Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation at Thr3 of H3 in prophase and its dephosphorylation during anaphase (11).

染色质结构的修饰在调节真核细胞的转录中扮演着重要的角色。由DNA围绕和八聚体组蛋白(H2A,H2B,H3和H4各两个)共同组成的核小体是染色质的主要组成(1)。核小体的组蛋白氨基酸尾端进行不同的转录后修饰,包括乙酰化,磷酸化,甲基化和泛素化(2-5)。这些修饰通过不同的刺激产生并且对转录因子能否接近染色质有着直接的影响,所以也影响着基因的表达(6)。在大多数的物种中组蛋白H2B主要在Lys5,,12,,15和20位点上发生乙酰化(4,7)。组蛋白H3主要是在Lys9,14,18,23,27和56位点上发生乙酰化。在某些物种里H3上的Lys9位点发生乙酰化并应该在组蛋白沉积和染色质组装中扮演着重要的角色(2,3)。组蛋白H3上Ser10,Ser28和Thr11位点的磷酸化在有丝分裂和无丝分裂中都与染色质的缩合紧密相连(8-10)。H3的Thr3位点的磷酸化在许多物种中都是高度保守的,是由kinase haspin所催化的。在哺乳动物中用磷酸化特异性的抗体做免疫组化显示H3的Thr3位点在有丝分裂的前期发生磷酸化,后期发生去磷酸化(11)。

  1. Workman, J.L. and Kingston, R.E. (1998) Annu Rev Biochem 67, 545-79.
  2. Hansen, J.C. et al. (1998) Biochemistry 37, 17637-41.
  3. Strahl, B.D. and Allis, C.D. (2000) Nature 403, 41-5.
  4. Cheung, P. et al. (2000) Cell 103, 263-71.
  5. Bernstein, B.E. and Schreiber, S.L. (2002) Chem Biol 9, 1167-73.
  6. Jaskelioff, M. and Peterson, C.L. (2003) Nat Cell Biol 5, 395-9.
  7. Thorne, A.W. et al. (1990) Eur J Biochem 193, 701-13.
  8. Hendzel, M.J. et al. (1997) Chromosoma 106, 348-60.
  9. Goto, H. et al. (1999) J Biol Chem 274, 25543-9.
  10. Preuss, U. et al. (2003) Nucleic Acids Res 31, 878-85.
  11. Dai, J. et al. (2005) Genes Dev 19, 472-88.
  12. Idikio, H.A. (2006) Anticancer Res 26, 4687-94.

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