Cell Signaling Technology

Product Pathways - Motif Antibodies

Nitro-Tyrosine Antibody #9691

general   motif   nitrotyrosine   substrate  

No. Size Price
9691S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
9691 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

Nitro-Tyrosine Antibody detects proteins and peptides containing nitro-tyrosine in a manner independent of the surrounding amino acid sequence. It is a valuable tool for identifying new nitrated proteins as well as for assaying protein nitration and measuring levels of nitrated proteins in tissues and samples. The antibody does not cross-react with unmodified tyrosine or with phospho-tyrosine. (U.S. Patent No's.: 6,441,140; 6,982,318; 7,259,022; 7,344,714; U.S.S.N. 11,484,485; and all foreign equivalents.)

Nitro-Tyrosine Antibody兔多抗可以识别包含硝基酪氨酸的蛋白或肽段,不受周围氨基酸序列影响。这是发现新的硝基蛋白、分析蛋白硝基化和测量组织样本中硝基蛋白含量的有用工具。此抗体不与未修饰的酪氨酸或磷酸化酪氨酸发生交叉反应。(美国专利号:6,441,140、6,982,318、7,259,022、7,344,714;U.S.S.N. 11,484,485;及所有国外相应专利)

Source / Purification

Polyclonal antibodies are produced by immunizing animals with synthetic nitro-tyrosine-containing peptides . Antibodies are purified by protein A and peptide affinity chromatography.

该多克隆抗体用合成的包含硝基化酪氨酸的肽段免疫动物制备。该抗体使用蛋白A和蛋白亲和层析纯化制备。

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines, untreated or treated with peroxynitrite, degraded peroxynitrite or pervanadate, using Phospho-Tyrosine Mouse mAb (P-Tyr-100) #9411 (upper) or Nitro-Tyrosine Antibody (lower).

对多个细胞系抽提物,未处理或过氧亚硝基阴离子处理适当时间,降解过氧亚硝基阴离子或过钒酸钠,使用Phospho-Tyrosine Mouse mAb (P-Tyr-100) #9411(上图)或Nitro-Tyrosine Antibody(下图)进行Western blot分析。

Background

Nitric oxide (NO) is implicated in carcinogenesis (1), chronic infection, inflammation (2) and neurodegeneration (3). High levels of both superoxide and nitric oxide in tissues interact to form peroxynitrite, a potent oxidant that can modify Tyr residues in proteins to form 3-nitro-tyrosine (4). Tyrosine nitration of mitochondrial manganese superoxide dismutase results in loss of enzymatic activity (4). The nitration of p53 at Tyr residues abolishes its capacity for binding to its DNA consensus sequence (5).

一氧化氮(NO)与肿瘤生成(1)、慢性感染、炎症(2)和神经退行性病变(3)有关。组织中高水平的过氧化物或一氧化氮相互作用形成过氧硝酸盐,它可以将蛋白中的酪氨酸残基转化为3-硝基-酪氨酸(4)。线粒体锰超氧化物歧化酶的酪氨酸硝基化可导致酶活性的丧失(4)。p53酪氨酸残基硝基化会消除其与DNA一致序列的结合能力(5)。

  1. Bentz, B.G. et al. (2000) Head Neck 22, 64-70.
  2. Jaiswal, M. et al. (2000) Cancer Res 60, 184-90.
  3. Olivenza, R. et al. (2000) J Neurochem 74, 785-91.
  4. MacMillan-Crow, L.A. et al. (1996) Proc Natl Acad Sci U S A 93, 11853-8.
  5. Chazotte-Aubert, L. et al. (2000) Biochem Biophys Res Commun 267, 609-13.

Application References

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Protocols

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

Use of Cell Signaling Technology (CST) Motif Antibodies within certain methods (e.g., U.S. Patents No. 7,198,896 and 7,300,753) may require a license from CST. For information regarding academic licensing terms please have your technology transfer office contact CST Legal Department at CST_ip@cellsignal.com. For information regarding commercial licensing terms please contact CST Pharma Services Department at ptmscan@cellsignal.com.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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