Cell Signaling Technology

Product Pathways - Chromatin Regulation / Epigenetics

Acetyl-Histone H3 (Lys9) (C5B11) Rabbit mAb (Alexa Fluor® 488 Conjugate) #9683

CellSimple   CellSimple and Cellular Assay  

No. Size Price
9683S 100 µl ( 50 tests ) ¥3,986.00 现货查询 购买询价
9683 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
F 1:50 Human,Mouse,Rat,Monkey,Zebrafish,C. elegans, Endogenous Rabbit IgG
IF-IC 1:400

Species cross-reactivity is determined by western blot.

Applications Key: F=Flow Cytometry, IF-IC=Immunofluorescence (Immunocytochemistry),

Homology

Species predicted to react based on 100% sequence homology: Rat, Monkey, S. cerevisiae,

Specificity / Sensitivity

Acetyl-Histone H3 (Lys9) (C5B11) Rabbit mAb (Alexa Fluor® 488 Conjugate) detects endogenous levels of histone H3 only when acetylated on Lys9. This antibody does not cross-react with other acetylated histones.

Acetyl-Histone H3 (Lys9) (C5B11) Rabbit mAb 兔单抗(Alexa Fluor® 488 Conjugate)能够检测仅在Lys9位点乙酰化的内源性histone H3的蛋白水平。该抗体不能与其它乙酰化的组蛋白发生交叉反应。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the amino terminus of histone H3 in which Lys9 is acetylated. This antibody was conjugated to Alexa Fluor® 488 under optimal conditions with an F/P ratio of 2-6.

通过合成的仅在Lys9位点乙酰化的人源histone H3蛋白氨基端相应的多肽片段去免疫动物从而制备出此单克隆抗体。该抗体以F/P比率2-6的最佳条件偶联了Alexa Fluor® 488。

Description

This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 488 fluorescent dye and tested in-house for direct flow cytometry and immunofluorescent analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Acetyl-Histone H3 (Lys9) (C5B11) Rabbit mAb #9649.

Cell Signaling Technology antibody偶联了Alexa Fluor® 488 fluorescent dye,并且用直标流式细胞检测法和免疫荧光分析法检测人类细胞。该抗体可以与非偶联的Acetyl-Histone H3 (Lys9) (C5B11) Rabbit mAb #9649兔单抗有相同物种的交叉反应。

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of HeLa cells, untreated (blue) or SAHA-treated (green), using Acetyl-Histone H3 (Lys9) (C5B11) Rabbit mAb (Alexa Fluor® 488 Conjugate).

使用Acetyl-Histone H3 (Lys9) (C5B11) Rabbit mAb兔单抗 (Alexa Fluor® 488 Conjugate),流式细胞仪分析未处理的HeLa细胞,细胞分为untreated (蓝色)或SAHA-treated (绿色)。

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells, treated with TSA #9950 (left) and untreated (right), using Acetyl-Histone H3 (Lys9) (C5B11) Rabbit mAb (Alexa Fluor® 488 Conjugate) (green). Actin filaments have been labeled with DY-554 phalloidin (red).

使用Acetyl-Histone H3 (Lys9) (C5B11) Rabbit mAb 兔单抗(Alexa Fluor® 488 Conjugate)(绿色),共聚焦免疫荧光分析HeLa细胞,细胞分为TSA #9950 treated (左图)和untreated (#9950;右图)。DY-554 phalloidin标记微丝蛋白(红色)。

Background

Modulation of chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin (1). The amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination (2-5). These modifications occur in response to various stimuli and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, gene expression (6). In most species, histone H2B is primarily acetylated at Lys5, 12, 15, and 20 (4,7). Histone H3 is primarily acetylated at Lys9, 14, 18, 23, 27, and 56. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (2,3). Phosphorylation at Ser10, Ser28, and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis (8-10). Phosphorylation at Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation at Thr3 of H3 in prophase and its dephosphorylation during anaphase (11).

染色质结构的修饰在调节真核细胞的转录中扮演着重要的角色。由DNA围绕和八聚体组蛋白(H2A,H2B,H3和H4各两个)共同组成的核小体是染色质的主要组成(1)。核小体的组蛋白氨基酸尾端进行不同的转录后修饰,包括乙酰化,磷酸化,甲基化和泛素化(2-5)。这些修饰通过不同的刺激产生并且对转录因子能否接近染色质有着直接的影响,所以也影响着基因的表达(6)。在大多数的物种中组蛋白H2B主要在Lys5,,12,,15和20位点上发生乙酰化(4,7)。组蛋白H3主要是在Lys9,14,18,23,27和56位点上发生乙酰化。在某些物种里H3上的Lys9位点发生乙酰化并应该在组蛋白沉积和染色质组装中扮演着重要的角色(2,3)。组蛋白H3上Ser10,Ser28和Thr11位点的磷酸化在有丝分裂和无丝分裂中都与染色质的缩合紧密相连(8-10)。H3的Thr3位点的磷酸化在许多物种中都是高度保守的,是由kinase haspin所催化的。在哺乳动物中用磷酸化特异性的抗体做免疫组化显示H3的Thr3位点在有丝分裂的前期发生磷酸化,后期发生去磷酸化(11)。

  1. Workman, J.L. and Kingston, R.E. (1998) Annu Rev Biochem 67, 545-79.
  2. Hansen, J.C. et al. (1998) Biochemistry 37, 17637-41.
  3. Strahl, B.D. and Allis, C.D. (2000) Nature 403, 41-5.
  4. Cheung, P. et al. (2000) Cell 103, 263-71.
  5. Bernstein, B.E. and Schreiber, S.L. (2002) Chem Biol 9, 1167-73.
  6. Jaskelioff, M. and Peterson, C.L. (2003) Nat Cell Biol 5, 395-9.
  7. Thorne, A.W. et al. (1990) Eur J Biochem 193, 701-13.
  8. Hendzel, M.J. et al. (1997) Chromosoma 106, 348-60.
  9. Goto, H. et al. (1999) J Biol Chem 274, 25543-9.
  10. Preuss, U. et al. (2003) Nucleic Acids Res 31, 878-85.
  11. Dai, J. et al. (2005) Genes Dev 19, 472-88.

Application References

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Protocols

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For Research Use Only. Not For Use In Diagnostic Procedures.

U.S. Patent No. 5,675,063.

The Alexa Fluor dye antibody conjugates in this product are sold under license from Life Technologies Corporation for research use only, except for use in combination with DNA microarrays. The Alexa Fluor® dyes (except for Alexa Fluor® 430 dye) are covered by pending and issued patents. Alexa Fluor® is a registered trademark of Molecular Probes, Inc.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

CellSimple is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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