Cell Signaling Technology

Product Pathways - Apoptosis

Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb #9664

active caspase 3   active caspase-3   anti caspase 3   anti caspase-3   Apopain   apoptosis   casp367   casp39   caspase 3   caspase-3   caspase3   CPP 32   CPP-32   CPP32   sc-22171   SCA-1   Yama Protein  

No. Size Price
9664L 300 µl ( 30 western blots ) ¥9,325.00 现货查询 购买询价
9664S 100 µl ( 10 western blots ) ¥3,780.00 现货查询 购买询价
9664T 20 µl ( 2 western blots ) ¥1,300.00 现货查询 购买询价
9664 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 17, 19 Rabbit IgG
IP 1:50
IHC-P 1:1000
F 1:6400
IF-IC 1:400
IHC-F 1:800

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin), F=Flow Cytometry, IF-IC=Immunofluorescence (Immunocytochemistry), IHC-F=Immunohistochemistry (Frozen),

Homology

Species predicted to react based on 100% sequence homology: Dog,

Specificity / Sensitivity

Cleaved Caspase-3 (Asp175) (5A1) Rabbit mAb detects endogenous levels of the large fragment (17/19 kDa) of activated caspase-3 resulting from cleavage adjacent to Asp175. This antibody does not recognize full length caspase-3 or other cleaved caspases.

Cleaved Caspase-3 (Asp175) (5A1) Rabbit mAb 兔单抗能够检测活化的邻近Asp175处剪切的caspase-3大片段(17/19 kDa)。该抗体不能识别全长的 caspase-3 和其它caspases的剪切产物。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to amino-terminal residues adjacent to Asp175 of human caspase-3.

该单克隆抗体是采用合成的人源caspases-3 Asp175 氨基末端序列相对应的肽段免疫动物后制备获得。

IP

IP

Immunoprecipitation of extracts from Jurkat cells, untreated or etoposide-treated (25uM, 5hrs), using Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb. Western blot was performed using the same antibody.

免疫沉淀分析Jurkat 细胞的提取物,未处理或 etoposide (依托泊苷)(25uM, 5hrs)处理,采用Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb 兔单抗,然后用同样抗体进行western blot 分析。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical staining of paraffin-embedded mouse embryo, showing cytoplasmic localization in apoptotic cells, using Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb.

免疫组化分析石蜡包埋小鼠胚胎,显示凋亡细胞中裂解的caspase-3定位胞浆,抗体采用 Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb 兔单抗。

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of Jurkat cells, untreated (blue) or treated with etoposide #2200 (green), using Cleaved Caspase-3(Asp175) (5A1E) Rabbit mAb compared to a nonspecific negative control antibody (red).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis using Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb on SignalSlide (TM) Cleaved Caspase-3 IHC Controls #8104 (paraffin-embedded Jurkat cell, untreated (left) or etoposide-treated (right)).

免疫组化分析,用 SignalSlide (TM) Cleaved Caspase-3 IHC Controls #8104(石蜡包埋的Jurkat 细胞,未处理(左)或 etoposide 处理(右)),采用 Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb 兔单抗。

Western Blotting

Western Blotting

Western blot analysis of extracts from C6 (rat), NIH/3T3 (mouse), and Jurkat (human) cells, untreated or treated with staurosporine (1uM, 3hrs) or etoposide (25uM, 5hrs) as indicated, using Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb.

Western blot 分析未处理或 staurosporine (星胞菌素) (1uM, 3hrs) 或 etoposide (依托泊苷)(25uM, 5hrs)处理的C6 (大鼠源)、NIH/3T3 (小鼠源)和Jurkat (人源) 细胞。抗体采用Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb 兔单抗。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded mouse embryo, using Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb in the presence of control peptide (left) or Cleaved Caspase-3 (Asp175) Blocking Peptide (#1050) (right).

免疫组化分析石蜡包埋的小鼠胚胎,抗体采用Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb 兔单抗,并用对照肽段(左图)或 Cleaved Caspase-3 (Asp175) 封闭肽段 (#1050) 对照(右图)。

IF-IC

IF-IC

Confocal immunofluorescent images of HT-29 cells, untreated (left) or Staurosporine treated (right) labeled with Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb#9953 (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

共聚焦免疫荧光分析未处理(左)或 Staurosporine (星胞菌素)处理(右)的HT-29 细胞,抗体采用 Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb 兔单抗#9953(绿色)。微丝用Alexa Fluor® 555 phalloidin(红色)标记。蓝色伪彩=DRAQ5® #4084 (DNA 荧光染料)。

IHC-F (frozen)

IHC-F (frozen)

Immunohistochemical analysis of frozen H1650 xenograft, using Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb.

免疫组化分析 H1650 xenograft 冰冻切片,抗体采用Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb 兔单抗。

Background

Caspase-3 (CPP-32, Apoptain, Yama, SCA-1) is a critical executioner of apoptosis, as it is either partially or totally responsible for the proteolytic cleavage of many key proteins such as the nuclear enzyme poly(ADP-ribose) polymerase (PARP) (1). Activation of caspase-3 requires proteolytic processing of its inactive zymogen into activated p17 and p12 fragments. Cleavage of caspase-3 requires aspartic acid at the P1 position (2).

Caspase-3 (CPP-32, Apoptain, Yama, SCA-1)是一个凋亡的关键执行者,因为它对许多关键的蛋白起着部分或全部的裂解作用,比如核酶多聚(ADP-ribose)聚合酶(PARP)(1)。caspase-3的活化需要经过其未激活的的酶原转变成活化的 p17 和 p12 片段的蛋白水解过程。caspase-3的剪切需要天门冬氨酸位于p1位点(2)。

  1. Fernandes-Alnemri, T. et al. (1994) J Biol Chem 269, 30761-4.
  2. Nicholson, D.W. et al. (1995) Nature 376, 37-43.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

U.S. Patent No. 5,675,063.

DRAQ5 is a registered trademark of Biostatus Limited.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

SignalStain is a trademark of Cell Signaling Technology, Inc.

Alexa Fluor is a registered trademark of Life Technologies Corporation.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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