Cell Signaling Technology

Product Pathways - Apoptosis

Caspase-3 Control Cell Extracts #9663

Apopain   casp3   casp367   casp39   caspase 3   caspase-3   caspase3   CPP 32   CPP-32   CPP32   Lysate   SCA-1   Yama Protein  

No. Size Price
9663S 100 µl ( 10 western blots ) ¥1,415.00 现货查询 购买询价
9663 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype

Species cross-reactivity is determined by western blot.

Applications Key:


Untreated Jurkat Control Cell Extracts: Untreated Jurkat cells are lysed in Chaps cell extract buffer and a cytoplasmic fraction is generated to serve as a negative control for caspase cleavage. 
Cytochrome c Treated Jurkat Cell Extracts: Untreated Jurkat cells are lysed in Chaps cell extract buffer and a cytoplasmic fraction is generated. Extracts are then treated with cytochrome c in vitro to generate a positive control for caspase cleavage.

未处理的Jurkat 对照细胞提取物:未经处理的Jurkat细胞溶解在Chaps细胞提取物缓冲液中,产生一种胞质段,作为caspase裂解的阴性对照。用 Cytochrome c 处理的 Jurkat 细胞提取物:未处理的 Jurkat 细胞 溶解在 Chaps 细胞提取物缓冲液中,产生一个细胞质片段。然后,在体外用 Cytochrome c 处理后的提取物可以作为 caspase 裂解产物的阳性对照。

Western Blotting

Western Blotting

Western blot analysis of Jurkat cell extracts untreated or treated with cytochrome c in vitro, showing full length and/or cleaved caspase-3 (upper) and cleaved caspase-3 Asp175 (lower), using Caspase-3 Antibody #9662 and Cleaved Caspase-3 (Asp175) Antibody #9661.


Western Blots: CST recommends boiling control cell extracts for two minutes prior to loading. Load 10 µl of each Jurkat control cell extract per lane.

Western Blots:建议电泳加样之前先对对照细胞提取物煮沸两分钟。每个泳道加入10μl的Jurkat对照细胞提取物。


Caspase-3 (CPP-32, Apoptain, Yama, SCA-1) is a critical executioner of apoptosis, as it is either partially or totally responsible for the proteolytic cleavage of many key proteins such as the nuclear enzyme poly(ADP-ribose) polymerase (PARP) (1). Activation of caspase-3 requires proteolytic processing of its inactive zymogen into activated p17 and p12 fragments. Cleavage of caspase-3 requires aspartic acid at the P1 position (2).

Caspase-3 (CPP-32, Apoptain, Yama, SCA-1)是一个凋亡的关键执行者,因为它对许多关键的蛋白起着部分或全部的裂解作用,比如核酶多聚(ADP-ribose)聚合酶(PARP)(1)。caspase-3的活化需要经过其未激活的的酶原转变成活化的 p17 和 p12 片段的蛋白水解过程。caspase-3的剪切需要天门冬氨酸位于p1位点(2)。

  1. Fernandes-Alnemri, T. et al. (1994) J Biol Chem 269, 30761-4.
  2. Nicholson, D.W. et al. (1995) Nature 376, 37-43.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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