Cell Signaling Technology

Product Pathways - Apoptosis

Caspase-3 Control Cell Extracts #9663

Apopain   casp3   casp367   casp39   caspase 3   caspase-3   caspase3   CPP 32   CPP-32   CPP32   Lysate   SCA-1   Yama Protein  

No. Size Price
9663S 100 µl ( 10 western blots ) ¥1,450.00 现货查询 购买询价 防伪查询
9663 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype

Species cross-reactivity is determined by western blot.

Applications Key:


Untreated Jurkat Control Cell Extracts: Untreated Jurkat cells are lysed in Chaps cell extract buffer and a cytoplasmic fraction is generated to serve as a negative control for caspase cleavage. 
Cytochrome c Treated Jurkat Cell Extracts: Untreated Jurkat cells are lysed in Chaps cell extract buffer and a cytoplasmic fraction is generated. Extracts are then treated with cytochrome c in vitro to generate a positive control for caspase cleavage.

未处理的Jurkat 对照细胞提取物:未经处理的Jurkat细胞溶解在Chaps细胞提取物缓冲液中,产生一种胞质段,作为caspase裂解的阴性对照。用 Cytochrome c 处理的 Jurkat 细胞提取物:未处理的 Jurkat 细胞 溶解在 Chaps 细胞提取物缓冲液中,产生一个细胞质片段。然后,在体外用 Cytochrome c 处理后的提取物可以作为 caspase 裂解产物的阳性对照。

Western Blotting

Western Blotting

Western blot analysis of Jurkat cell extracts untreated or treated with cytochrome c in vitro, showing full length and/or cleaved caspase-3 (upper) and cleaved caspase-3 Asp175 (lower), using Caspase-3 Antibody #9662 and Cleaved Caspase-3 (Asp175) Antibody #9661.


Western Blots: CST recommends boiling control cell extracts for two minutes prior to loading. Load 10 µl of each Jurkat control cell extract per lane.

Western Blots:建议电泳加样之前先对对照细胞提取物煮沸两分钟。每个泳道加入10μl的Jurkat对照细胞提取物。


Caspase-3 (CPP-32, Apoptain, Yama, SCA-1) is a critical executioner of apoptosis, as it is either partially or totally responsible for the proteolytic cleavage of many key proteins such as the nuclear enzyme poly(ADP-ribose) polymerase (PARP) (1). Activation of caspase-3 requires proteolytic processing of its inactive zymogen into activated p17 and p12 fragments. Cleavage of caspase-3 requires aspartic acid at the P1 position (2).

Caspase-3 (CPP-32, Apoptain, Yama, SCA-1)是一个凋亡的关键执行者,因为它对许多关键的蛋白起着部分或全部的裂解作用,比如核酶多聚(ADP-ribose)聚合酶(PARP)(1)。caspase-3的活化需要经过其未激活的的酶原转变成活化的 p17 和 p12 片段的蛋白水解过程。caspase-3的剪切需要天门冬氨酸位于p1位点(2)。

  1. Fernandes-Alnemri, T. et al. (1994) J Biol Chem 269, 30761-4.
  2. Nicholson, D.W. et al. (1995) Nature 376, 37-43.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

用户评论 --- 共 0


我要参与评论 :