Cell Signaling Technology

Product Pathways - Apoptosis

Cleaved Caspase-3 (Asp175) Antibody #9661

active caspase 3   active caspase-3   anti caspase 3   anti caspase-3   Apopain   apoptosis   casp367   casp39   caspase 3   caspase-3   caspase3   cleaved caspase 3   CPP 32   CPP-32   CPP32   sc-22171   SCA-1   Yama Protein  

No. Size Price
9661L 300 µl ( 30 western blots ) ¥8,792.00 现货查询 购买询价
9661S 100 µl ( 10 western blots ) ¥3,780.00 现货查询 购买询价
9661T 20 µl ( 2 western blots ) ¥1,300.00 现货查询 购买询价
9661 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 17, 19 Rabbit
IP 1:100
IHC-P 1:300
F 1:800
IF-IC 1:400
IHC-F 1:600

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin), F=Flow Cytometry, IF-IC=Immunofluorescence (Immunocytochemistry), IHC-F=Immunohistochemistry (Frozen),


Species predicted to react based on 100% sequence homology: Bovine, Dog, Pig,

Specificity / Sensitivity

Cleaved Caspase-3 (Asp175) Antibody detects endogenous levels of the large fragment (17/19 kDa) of activated caspase-3 resulting from cleavage adjacent to Asp175. This antibody does not recognize full length caspase-3 or other cleaved caspases.

该抗体能够检测 活化的Asp175 附近剪切的caspase-3大片段(17/19 kDa)。该抗体不能识别全长的caspase-3和其他caspase裂解产物。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino-terminal residues adjacent to (Asp175) in human caspase-3. Antibodies are purified by protein A and peptide affinity chromatography.

该多克隆抗体是采用合成的人源caspases Asp175氨基酸氨基末端序列相对应的肽段免疫动物后制备获得。抗体通过蛋白A和肽段亲和层析而纯化。

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa, NIH/3T3 and C6 cells untreated, staurosporine-treated (3hrs, 1 µM in vivo) or cytochrome c-treated (1hr, 0.25 mg/ml in vitro), using Caspase-3 Antibody #9662 (upper) or Cleaved Caspase-3 (Asp175) Antibody (lower).

Western blot 检测Hela、NIH/3T3 和 C6 细胞的细胞提取物,未处理和 staurosporine(星胞菌素)处理(3hrs, 1 µM,体内) 或cytochrome c(细胞色素C)处理 (1hr, 0.25 mg/ml,体外),使用 Caspase-3 Antibody #9662 (上图) 和 Cleaved Caspase-3 (Asp175) Antibody (下图)。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human tonsil, showing cytoplasmic and perinuclear localization in apoptotic cells (low and high magnification), using Cleaved Caspase-3 (Asp175) Antibody.

免疫组化检测石蜡包埋的人扁桃体切片,显示凋亡细胞中的胞浆和核周定位(低倍和高倍),使用抗体为Cleaved Caspase-3 (Asp175) Antibody.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of Jurkat cells, untreated (blue) or etoposide-treated (green), using Cleaved Caspase-3 (Asp175) Antibody compared to a nonspecific negative control antibody (red).<

流式细胞术分析 Jurkat cells,未处理 (蓝色) or etoposide 处理 (绿色), 使用抗体 为 Cleaved Caspase-3 (Asp175) Antibody,非特异性抗体阴性作为对照(红色).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded mouse embryo, using Cleaved Caspase-3 (Asp175) Antibody preincubated with control peptide (left) or Cleaved Caspase-3 (Asp175) Blocking Peptide #1050 (right).

免疫组化检测石蜡包埋的小鼠胚胎,使用抗体为 Cleaved Caspase-3 (Asp175) Antibody,对照肽段 (左) or Cleaved Caspase-3 (Asp175) 阻断肽段 #1050 (右)孵育.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis using Cleaved caspase-3 (Asp175) antibody on SignalSlideTM Cleaved Caspase-3 IHC controls #8104 (paraffin-embedded Jurkat cells, untreated (left) or etoposide-treated (right)).

免疫组化显示:使用抗体是 Cleaved caspase-3 (Asp175) antibody on SignalSlideTM Cleaved Caspase-3 IHC controls #8104 (石蜡包埋 Jurkat cells, 未处理(左) or etoposide(依托泊苷)处理 (右)).



Confocal immunofluorescent images of HT-29 cells, untreated (left) or Staurosporine #9953 treated (right), labeled with Cleaved Caspase-3 (Asp175) Antibody (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5® (fluorescent DNA dye).

共聚焦免疫荧光显示HT-29 cells 图片,未处理(左)或 Staurosporine (星胞菌素)处理(右)用 Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb 标记#9953标记(绿色)。微丝用Alexa Fluor® 555 phalloidin(鬼比环肽)(红色)。蓝色伪彩=DRAQ5®(DNA荧光染料)。

IHC-F (frozen)

IHC-F (frozen)

Immunohistochemical analysis of frozen H1650 xenograft section, using Cleaved Caspase-3 (Asp175) Antibody.

免疫组化分析H1650移植物冰冻切片,使用抗体Cleaved Caspase-3 (Asp175) Antibody.


Caspase-3 (CPP-32, Apoptain, Yama, SCA-1) is a critical executioner of apoptosis, as it is either partially or totally responsible for the proteolytic cleavage of many key proteins such as the nuclear enzyme poly(ADP-ribose) polymerase (PARP) (1). Activation of caspase-3 requires proteolytic processing of its inactive zymogen into activated p17 and p12 fragments. Cleavage of caspase-3 requires aspartic acid at the P1 position (2).

Caspase-3 (CPP-32, Apoptain, Yama, SCA-1)是一个凋亡的关键执行者,因为它对许多关键的蛋白起着部分或全部的裂解作用,比如核酶多聚(ADP-ribose)聚合酶(PARP)(1)。caspase-3的活化需要经过其未激活的的酶原转变成活化的 p17 和 p12 片段的蛋白水解过程。caspase-3的剪切需要天门冬氨酸位于p1位点(2)。

  1. Fernandes-Alnemri, T. et al. (1994) J Biol Chem 269, 30761-4.
  2. Nicholson, D.W. et al. (1995) Nature 376, 37-43.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

DRAQ5 is a registered trademark of Biostatus Limited.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Alexa Fluor is a registered trademark of Life Technologies Corporation.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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