Cell Signaling Technology

Product Pathways - Motif Antibodies

Phospho-Akt Substrate (RXXS*/T*) (110B7E) Rabbit mAb (Sepharose® Bead Conjugate) #9646

Akt   akt1   akt2   akt3   motif   RXRXXS   RXRXXT   substrate  

No. Size Price
9646S 400 µl ( 40 immunoprecipitations ) ¥3,986.00 现货查询 购买询价
9646 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
IP 1:20 Mouse,D. melanogaster,All Species Expected, Endogenous Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: IP=Immunoprecipitation,

Specificity / Sensitivity

Phospho-(Ser/Thr) Akt Substrate Motif (RXXS*/T*) (110B7) Rabbit mAb (Sepharose Bead Conjugate) recognizes peptides and proteins containing phospho-serine/threonine preceded by arginine at the -3 position. There is some preference observed for peptides that contain phospho-serine/threonine preceded by arginine at both positions -5 and -3. (U.S. Patent No's.: 6,441,140; 6,982,318; 7,259,022; 7,344,714; U.S.S.N. 11,484,485; and all foreign equivalents.)

Phospho-(Ser/Thr) Akt Substrate Motif (RXXS*/T*) (110B7) Rabbit mAb (Sepharose Bead Conjugate)琼脂糖珠偶联兔单抗识别包含-3位上是精氨酸的磷酸化丝氨酸/苏氨酸的肽段和蛋白。此抗体与包含-5、-3位上是精氨酸的磷酸化丝氨酸/苏氨酸的肽段有偏好的交叉反应。(美国专利号:6,441,140、6,982,318、7,259,022、7,344,714;U.S.S.N. 11,484,485;及所有国外相应专利)

Source / Purification

Monoclonal antibody is produced by immunizing animals with synthetic phospho-Akt substrate peptides.

该单克隆抗体用合成的磷酸化Akt底物免疫动物制备。

Description

This Cell Signaling Technology antibody is immobilized via covalent binding of primary amino groups to N-hydroxysuccinimide (NHS)-activated sepharose beads. Phospho-Akt Substrate (RXXS*/T*) (110B7E) Rabbit mAb (Sepharose Bead Conjugate) is useful for the immunoprecipitation of phosphorylated Akt substrate proteins.

CST公司生产的此抗体通过第一个氨基基团共价结合到N-羟基琥珀酰亚胺(NHS)活化的琼脂糖珠上而固定。Phospho-Akt Substrate (RXXS*/T*) (110B7E) Rabbit mAb (Sepharose Bead Conjugate)在磷酸化Akt底物蛋白的免疫沉淀分析中有用。

IP

IP

Immunoprecipitation of NIH/3T3 cell lysates, untreated or PDGF-treated, using Phospho-Akt Substrate (RXXS*/T*) (110B7E) Rabbit mAb (Sepharose Bead Conjugate) compared to supernatants collected after immunoprecipitation from the same lysate. The western blot was probed using Phospho-Akt Substrate (RXXS*/T*) (110B7E) Rabbit mAb #9614.

NIH/3T3裂解液,未处理或PDGF处理,使用Phospho-Akt Substrate (RXXS*/T*) (110B7E) Rabbit mAb (Sepharose Bead Conjugate)进行免疫共沉淀分析,并与共沉淀后裂解液的上清液进行对照。Western blot使用Phospho-Akt Substrate (RXXS*/T*) (110B7E) Rabbit mAb #9614。

Background

An important class of kinases, referred to as Arg-directed kinases or AGC-family kinases, includes cAMP-dependent protein kinase (PKA), cGMP-dependent protein kinase (PKG), protein kinase C, Akt, and RSK. These kinases share a substrate specificity characterized by Arg at position -3 relative to the phosphorylated Ser or Thr (1,2). Akt plays a central role in mediating critical cellular responses including cell growth and survival, angiogenesis, and transcriptional regulation (3-5). While a number of Akt substrates are known (such as GSK-3, Bad, and caspase-9) many important substrates await discovery. Akt phosphorylates substrates only at Ser/Thr in a conserved motif characterized by Arg at positions -5 and -3 (6). Phospho-Akt substrate-specific antibodies from CST are powerful tools for investigating the regulation of phosphorylation by Akt and other Arg-directed kinases, as well as for high throughput kinase drug discovery.

精氨酸导向的激酶或AGC家族激酶是很重要的激酶,包括cAMP依赖的蛋白激酶(PKA)、cGMP依赖的蛋白激酶(PKG)、蛋白激酶C、Akt和RSK。这些激酶有相同的底物特异性,-3位上有精氨酸的丝氨酸和苏氨酸(1,2)。Akt在介导细胞内关键反应中扮演重要角色,包括细胞生长和生存、血管生成和转录调节(3-5)。目前已经发现一些Akt底物(如GSK-3、Bad、caspase-9),但还有很多重要底物等待发现。Akt磷酸化保守序列-5、-3位是精氨酸的丝氨酸/苏氨酸(6)。磷酸化Akt底物特异性抗体由Cell Signaling Technology公司研发,用于研究Akt磷酸化调节和精氨酸导向的激酶,也可以用于高通量药物发现。

  1. Montminy, M. (1997) Annu Rev Biochem 66, 807-22.
  2. Pearson, R.B. and Kemp, B.E. (1991) Methods Enzymol 200, 62-81.
  3. Marte, B.M. and Downward, J. (1997) Trends Biochem Sci 22, 355-8.
  4. Jiang, B.H. et al. (2000) Proc Natl Acad Sci USA 97, 1749-53.
  5. Scheid, M.P. and Woodgett, J.R. (2000) Curr Biol 10, R191-4.
  6. Alessi, D.R. et al. (1996) FEBS Lett 399, 333-8.

Application References

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Protocols

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

U.S. Patent No. 5,675,063.

Use of Cell Signaling Technology (CST) Motif Antibodies within certain methods (e.g., U.S. Patents No. 7,198,896 and 7,300,753) may require a license from CST. For information regarding academic licensing terms please have your technology transfer office contact CST Legal Department at CST_ip@cellsignal.com. For information regarding commercial licensing terms please contact CST Pharma Services Department at ptmscan@cellsignal.com.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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