Cell Signaling Technology

Product Pathways - Motif Antibodies

Phospho-(Ser/Thr) PDK1 Docking Motif (18A2) Mouse mAb #9634

general   motif   PBS   PDK   PDK1   phospho serine   phospho-serine   phosphoserine   PKB kinase   substrate  

No. Size Price
9634S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
9634 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,All Species Expected, Endogenous Mouse IgG2a
IP 1:20
E-P 1:1000

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, E-P=Peptide ELISA (DELFIA),

Specificity / Sensitivity

Phospho-(Ser/Thr) PDK1 Docking Motif (18A2) Mouse mAb detects phosphorylated serine or threonine that is surrounded by tyrosine or phenylalanine at the -1 and +1 positions and phenylalanine at the -4 position. It also recognizes peptides containing lysine instead of phenylalanine at the -4 position. This antibody does not cross-react with the nonphosphorylated PDK1 docking motifs or with other phosphorylated motifs. This antibody detects endogenous levels of phosphorylated proteins containing PDK1 docking motif, including phospho-Akt. (U.S. Patent No's.: 6,441,140; 6,982,318; 7,259,022; 7,344,714; U.S.S.N. 11,484,485; and all foreign equivalents.)

Phospho-(Ser/Thr) PDK1 Docking Motif (18A2) Mouse mAb鼠单抗识别-1、+1位是酪氨酸或苯丙氨酸,和-4位是苯丙氨酸的磷酸化丝氨酸或苏氨酸。它也能识别包含-4位不是苯丙氨酸而是赖氨酸的肽段。此抗体不与相应的非磷酸化PDK1结合基序或其它磷酸化基序发生交叉反应。该抗体识别内源性的含PDK1结合基序的磷酸化蛋白,包括磷酸化Akt。(美国专利号:6,441,140、6,982,318、7,259,022、7,344,714;U.S.S.N. 11,484,485;及所有国外相应专利)

Source / Purification

Monoclonal antibody is produced by immunizing animals with peptides containing the PDK1 docking motif.

该单克隆抗体用含PDK1结合基序的肽段免疫动物制备。

Western Blotting

Western Blotting

Western blot analysis of extracts from A431 cells, untreated or calyculin A-treated (0.1 µM for 30 minutes prior to lysis), using Phospho-(Ser/Thr) PDK1 Docking Motif (18A2) Mouse mAb.

对未处理或0.1uM calyculin A裂解前处理30分钟的A431细胞抽提液使用Phospho-(Ser/Thr) PDK1 Docking Motif (18A2) Mouse mAb进行Western blot分析。

IP

IP

Immunoprecipitation of extracts from NIH/3T3 cells, untreated or treated with 100 ng/ml PDGF for 20 minutes prior to lysis, using Phospho-(Ser/Thr) Docking Motif (18A2) Mouse mAb and Akt Antibody #9272. Western blots were performed using Phospho-(Ser/Thr) Docking Motif (18A2) Mouse mAb (upper) or Akt Antibody #9272 (lower).

对未处理或100 ng/ml PDGF裂解前处理20分钟的NIH/3T3细胞抽提液使用Phospho-(Ser/Thr) Docking Motif (18A2) Mouse mAb and Akt Antibody #9272进行Western blot分析。使用Phospho-(Ser/Thr) Docking Motif (18A2) Mouse mAb(上)或Akt Antibody #9272(下)进行Western blot分析。

ELISA-Peptide

ELISA-Peptide

Phospho-(Ser/Thr) PDK1 Docking Motif (18A2) Mouse mAb ELISA Assay: Signal-to-noise ratio of phospho- versus nonphospho-peptides. (T* and S* denote phosphorylated threonine and serine.)

Phospho-(Ser/Thr) PDK1 Docking Motif (18A2) Mouse mAb ELISA分析:磷酸化和非磷酸化的信噪比。(T*和S*代表磷酸化的苏氨酸和丝氨酸)

Background

A hallmark of signal transduction pathways is the reversible phosphorylation of serine and threonine residues within specific sequences, or motifs, in target proteins. Specific signaling motifs include not only sequences that are recognized by protein kinases (1), but also those that are recognized by phosphorylation-dependent binding proteins such as 14-3-3 (2). These modular phosphoprotein interacting domains are critical elements in modulating, directing and amplifying intracellular communications. CST has pioneered the development of phospho-motif specific antibodies, which are invaluable tools for probing the complexity of phospho-regulatory pathways.

目标蛋白特定序列、基序可逆的丝氨酸和苏氨酸残基磷酸化是重要的信号转导通路的标志。特异的信号基序不仅包括可以被蛋白激酶识别的序列(1),也包括那些能够被磷酸化依赖结合蛋白如14-3-3识别的序列(2)。这些模块的磷酸化互作结构域对调节、定向和放大胞内通讯至关重要。CST在开发磷酸化基序特异性抗体领域遥遥领先,为识别磷酸化调节通路的复杂性提供了非常珍贵的工具。

Many critical protein kinases can be regulated by phosphorylation at a specific serine or threonine in a hydrophobic motif (3). For example, Akt, a kinase that regulates cell survival, is activated by phosphorylation at Ser473, a site preceded by Phe at -4 and -1 and followed by Tyr at +1 (4). RSK2, p70 S6 kinase and certain PKC isoforms also contain a similar consensus phosphorylation motif. Phosphorylation of these motifs is required for binding to 3-phosphoinositide-dependent kinase 1 (PDK1) (5-7). Phospho-(Ser/Thr) PDK1 Docking Motif (18A2) Monoclonal Antibody is a powerful tool for the characterization of phosphorylated PDK1 docking motifs and the identification of new proteins with PDK1 docking motifs.

许多重要的蛋白激酶都能被疏水基序中特异性的丝氨酸、苏氨酸磷酸化所调节(3)。例如,Akt这种激酶可以调节细胞生存,其因在-4、-1位苯丙氨酸之后、+1位酪氨酸之前的Ser473位点的磷酸化而激活(4)。RSK2、p70 S6激酶和某些PKC亚型也包含类似的一致的磷酸化位点。这些基序的磷酸化为结合3-磷酸肌醇依赖性蛋白激酶1(PDK1)所必需(5-7)。Phospho-(Ser/Thr) PDK1 Docking Motif (18A2) Monoclonal Antibody是分析磷酸化PDK1结合基序和发现包含PDK1结合基序蛋白的有力工具。

  1. Pinna, L.A. and Ruzzene, M. (1996) Biochim Biophys Acta 1314, 191-225.
  2. Yaffe, M.B. and Elia, A.E. (2001) Curr Opin Cell Biol 13, 131-8.
  3. Vanhaesebroeck, B. and Alessi, D.R. (2000) Biochem J 346 Pt 3, 561-76.
  4. Alessi, D.R. et al. (1996) EMBO J 15, 6541-51.
  5. Frödin, M. et al. (2000) EMBO J 19, 2924-34.
  6. Balendran, A. et al. (1999) J Biol Chem 274, 37400-6.
  7. Balendran, A. et al. (2000) J Biol Chem 275, 20806-13.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Use of Cell Signaling Technology (CST) Motif Antibodies within certain methods (e.g., U.S. Patents No. 7,198,896 and 7,300,753) may require a license from CST. For information regarding academic licensing terms please have your technology transfer office contact CST Legal Department at CST_ip@cellsignal.com. For information regarding commercial licensing terms please contact CST Pharma Services Department at ptmscan@cellsignal.com.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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