Cell Signaling Technology

Product Pathways - Neuroscience

Phospho-Tau (Ser396) (PHF13) Mouse mAb #9632

Mapt   Microtubule-associated protein tau   Mtapt   Neurofibrillary tangle protein   Paired helical filament-tau   PHF-tau  

No. Size Price
9632S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
9632 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Mouse,Rat, Endogenous 50-80 Mouse IgG2b

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Homology

Species predicted to react based on 100% sequence homology: Human,

Specificity / Sensitivity

Phospho-Tau (Ser396) (PHF13) Mouse mAb detects endogenous levels of Tau only when phosphorylated at serine 396.

Phospho-Tau (Ser396) (PHF13) Mouse mAb 鼠单抗可识别内源性的Ser396磷酸化的Tau蛋白。

Source / Purification

Monoclonal antibody is produced by immunizing animals with human purified Tau (Hoffmann et al., 1997).

该单克隆抗体用纯化的人类Tau蛋白(Hoffmann et al., 1997)免疫动物而制成。

Western Blotting

Western Blotting

Western blot analysis of extracts from mouse brain and CAD cells, using Phospho-Tau (Ser396) (PHF13) Mouse mAb. The phospho-specificity of the antibody was verified by peptide blocking using no peptide, phospho-peptide or nonphospho-peptide.

Western blot分析小鼠大脑和CAD细胞,使用的抗体是Phospho-Tau (Ser396) (PHF13) Mouse mAb鼠单抗。抗体的磷酸化特异性通过使用对照、磷酸化多肽或非磷酸化多肽进行封闭实验而验证。

Background

Tau is a heterogeneous microtubule-associated protein that promotes and stabilizes microtubule assembly, especially in axons. Six isoforms with different amino-terminal inserts and different numbers of tandem repeats near the carboxy-terminus have been identified, and tau is hyperphosphorylated at approximately 25 sites by ERK, GSK-3 and CDK5 (1-2). Phosphorylation decreases the ability of tau to bind to microtubules. Neurofibrillary tangles are a major hallmark of Alzheimer's disease and these tangles are bundles of paired helical filaments composed of hyperphosphorylated tau. In particular, phosphorylation of Ser396 by GSK-3 or CDK5 destabilizes microtubules in Alzheimer's disease. Furthermore, inclusions of tau are found in a number of other neurodegenerative diseases, collectively known as tauopathies (1,3).

Tau是微管相关蛋白异构物,促进和稳定微管装配,尤其是在轴突中。其中有六个有不同的氨基末端的异构体插入,有不同数量的羧基末端串联重复序列已被识别,tau蛋白可被ERK、GSK-3和CDK5在大约25个位点处被过度磷酸化(1-2)。Tau蛋白磷酸化可降低其与微观的结合能力。神经纤维缠结是阿尔茨海默氏症的一个主要标志,这些缠结是由过度磷酸化tau蛋白组成的成对螺旋细丝束。GSK-3或CDK5对Tau蛋白Ser396的磷酸化可以导致微管的破坏,特别是在阿尔茨海默氏症中。此外,Tau蛋白包涵体还在其它神经退行性疾病中被发现,这些疾病被统称为Tau蛋白病。

  1. Johnson, G.V. and Stoothoff, W.H. (2004) J Cell Sci 117, 5721-9.
  2. Hanger, D.P. et al. (1998) J Neurochem 71, 2465-76.
  3. Bramblett, G.T. et al. (1993) Neuron 10, 1089-99.

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