Cell Signaling Technology

Product Pathways - Motif Antibodies

Phospho-(Ser/Thr) Phe Antibody #9631

general   motif   PBS   SF   substrate   TF  

No. Size Price
9631S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
9631 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,All Species Expected, Endogenous Rabbit
IP 1:100
E-P 1:1000

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, E-P=Peptide ELISA (DELFIA),

Specificity / Sensitivity

Phospho-(Ser/Thr) Phe Antibody detects phospho-serine or threonine in the context of tyrosine, tryptophan or phenylalanine at the -1 position or phenylalanine at the +1 position. The antibody does not cross-react with the nonphosphorylated form of these motifs, nor does it cross-react with other phospho-serine/threonine-containing proteins and peptides. (U.S. Patent No's.: 6,441,140; 6,982,318; 7,259,022; 7,344,714; U.S.S.N. 11,484,485; and all foreign equivalents.)

Phospho-(Ser/Thr) Phe Antibody识别-1位是酪氨酸、色氨酸或苯丙氨酸,或+1位是苯丙氨酸的磷酸化丝氨酸或苏氨酸。此抗体不与相应的非磷酸化的基序,或其它包含磷酸化丝氨酸/苏氨酸的肽段或蛋白发生交叉反应。(美国专利号:6,441,140、6,982,318、7,259,022、7,344,714;U.S.S.N. 11,484,485;及其对应的国外相应专利)

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phospho-serine/threonine-phenylalanine-containing peptide. Antibodies are purified by protein A and peptide affinity chromatography.

该多克隆抗体用人工合成的包含磷酸化丝氨酸/苏氨酸-苯丙氨酸的肽段免疫动物制备。该抗体使用蛋白A和蛋白亲和层析纯化得到。

ELISA-Peptide

ELISA-Peptide

Phospho-(Ser/Thr) Phe Antibody ELISAs: Signal to noise ratio of phospho- versus nonphospho-peptides in which the phosphorylation site is adjacent to phenylalanine, tyrosine or tryptophan. (T* and S* denote phosphorylated threonine and serine.)

Phospho-(Ser/Thr) Phe Antibody ELISA分析:磷酸化和非磷酸化多肽的信噪比,磷酸化位点毗邻苯丙氨酸、酪氨酸和色氨酸。(T*和S*代表磷酸化的苏氨酸和丝氨酸)

Western Blotting

Western Blotting

Western blot analysis of extractss from A431 cells, untreated or calyculin A-treated, using Phospho-(Ser/Thr) Phe Antibody.

对未处理或calyculin A处理的A431细胞抽提液使用Phospho-(Ser/Thr) Phe Antibody进行Western blot分析。

Background

A hallmark of signal transduction pathways is the reversible phosphorylation of serine and threonine residues within specific sequences, or motifs, in target proteins. Specific signaling motifs include not only sequences that are recognized by protein kinases (1), but also those that are recognized by phosphorylation-dependent binding proteins such as 14-3-3 (2). These modular phosphoprotein interacting domains are critical elements in modulating, directing and amplifying intracellular communications. CST has pioneered the development of phospho-motif specific antibodies, which are invaluable tools for probing the complexity of phospho-regulatory pathways.

目标蛋白特定序列和基序的可逆丝氨酸和苏氨酸残基磷酸化是信号转导通路的标志。特异的信号基序不仅包括蛋白激酶可以识别的序列(1),也包括那些能够被磷酸化依赖结合蛋白如14-3-3识别的序列(2)。这些基序的磷酸化互作结构域对调节、定向和放大胞内通讯至关重要。CST在开发磷酸化基序特异性抗体领域遥遥领先,为识别磷酸化调节通路的复杂性提供了非常重要的工具。

Many critical protein kinases can be regulated by phosphorylation at a specific serine or threonine surrounded by phenylalanine or tyrosine. For example, Akt, a kinase that regulates cell survival, is activated by phosphorylation at Ser473, a site surrounded by phenylalanine and tyrosine (3). RSK1, p70S6K, and certain PKC isoforms also contain a similar consensus phosphorylation site. Phosphorylation of these sites is required for kinase activity (4,5). The Phospho-(Ser/Thr) Phe Antibody is a powerful tool for discovery of new proteins containing this important regulatory motif.

通过特异性的磷酸化苯丙氨酸或酪氨酸周围的丝氨酸、苏氨酸磷酸化可以调控许多重要的蛋白激酶。例如,Akt这种可以调节细胞生存的激酶,通过苯丙氨酸和酪氨酸周围的Ser473位点的磷酸化被激活(3)。RSK1、p70S6K和某些PKC亚型也包含类似的一致的磷酸化位点。这些位点磷酸化是激酶活性调节所必需(4,5)。Phospho-(Ser/Thr) Phe Antibody是发现包含这种重要调节基序新蛋白的有力工具。

  1. Pinna, L.A. and Ruzzene, M. (1996) Biochim Biophys Acta 1314, 191-225.
  2. Yaffe, M.B. and Elia, A.E. (2001) Curr Opin Cell Biol 13, 131-8.
  3. Alessi, D.R. et al. (1996) EMBO J 15, 6541-51.
  4. Dalby, K.N. et al. (1998) J Biol Chem 273, 1496-505.
  5. Keranen, L.M. et al. (1995) Curr Biol 5, 1394-1403.

Application References

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Protocols

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

Use of Cell Signaling Technology (CST) Motif Antibodies within certain methods (e.g., U.S. Patents No. 7,198,896 and 7,300,753) may require a license from CST. For information regarding academic licensing terms please have your technology transfer office contact CST Legal Department at CST_ip@cellsignal.com. For information regarding commercial licensing terms please contact CST Pharma Services Department at ptmscan@cellsignal.com.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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